Regulation of gene expression and genome organization by small RNAs

小RNA调控基因表达和基因组组织

• 批准号: 10202138
• 负责人: Carolyn Marie Phillips
• 金额: $ 44.14万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10202138
• 关键词: Affect; Binding Proteins; Caenorhabditis elegans; Cells; Cellular biology; Congenital Abnormality; Cytoplasmic Granules; Developmental Biology; Disease; Eukaryota; Gene Expression; Gene Expression Regulation; Generations; Genes; Genetic; Genetic Transcription; Genome; Germ Cells; Goals; Human; Human Biology; Human Development; Infertility; Malignant Neoplasms; Messenger RNA; Methylation; Microscopy; Nucleotides; Pathway interactions; Phase; Phosphorylation; Phylogeny; Play; Post-Transcriptional Regulation; Post-Translational Protein Processing; Proteins; RNA; RNA Degradation; RNA Interference; Regulator Genes; Regulatory Pathway; Role; Small RNA; Sorting - Cell Movement; Specificity; Structure; System; Time; Transcriptional Regulation; Translational Repression; Work; experimental study; genome integrity; human disease; tool

RNA silencing is a gene regulatory mechanism by which small RNAs (18-30 nucleotides) and their Argonaute protein co-factors modulate the expression of both foreign and endogenous mRNAs. Small RNAs play a critical role in maintaining proper gene expression by identifying fully or partially complementary mRNAs and targeting them for either transcriptional or post-transcriptional regulation. The role of RNA silencing in gene regulatory pathways is conserved across most eukaryotes and is thus of fundamental importance to the developmental and cellular biology of humans. C. elegans is an excellent system to study RNA silencing because of its short generation time, its transparency which is ideal for microscopy, and the powerful genetic tools available for genome manipulation. In C. elegans germ cells, proteins involved in RNA silencing are organized into sub- compartments of perinuclear condensates, with each sub-compartment playing a unique and critical role. Many protein factors have been identified that localize to these structures, yet we know little about how they are organized and assembled. In the first part of this proposal, we will identify factors and conditions that promote assembly of RNA silencing components into perinuclear condensates. We will further identify the RNAs in each sub-compartment, with the ultimate goal of dissecting the trajectory of a targeted mRNA through each of its phases: beginning with transcription, continuing through this assemblage of perinuclear condensates, and ultimately with translational repression or degradation by the RNA silencing pathway. Because there are ~27 C. elegans Argonaute proteins, many of which colocalize at P granules, we will next identify the factors contributing to sorting of small RNA into the appropriate Argonaute proteins. This sorting is critical because many Argonaute proteins bind different small RNAs from one another, target distinct groups of mRNAs, and can have very different regulatory effects on these target mRNAs. Finally, we will focus on how protein modifications such as phosphorylation and methylation can regulate RNA silencing pathways, including affecting localization, interacting partners, and dynamics of Argonaute proteins and other key factors. Together, the proposed experiments will uncover the key details of how RNA silencing pathways are organized, how the specificity of the pathways is defined, and what mechanisms regulate these pathways. This work will lead to a better understanding of how small RNAs modulate gene expression in healthy cells, which can ultimately be applied to discerning how changes in these pathways cause misregulation of genes in humans and transition to a disease state.

RNA沉默是一种小RNA（18-30个核苷酸）及其Argonaute的基因调控机制