RNAse functions in post-transcriptional gene regulation

项目总结 所有生命形式都必须维持一个动态平衡的基因表达程序，这一点是实现的 在基因表达的许多不同步骤，包括通过特定的降解 MRNAs和非编码RNA。许多进化上保守的RNA加工 酶介导这些关键的转录后事件，对内切酶具有重要作用 和外切核酸酶。许多遗传性疾病是由内切酶基因突变引起的。 或外核糖核酸酶基因，进一步强调了它们对人类健康的重要性。 真核生物使用核酸外切酶来降解大多数细胞内的mRNAs和5‘ 以及许多不同的ncRNA的3‘端处理。此外，核酸内切酶靶标 作为mRNA降解和其他核酸内切酶启动步骤的特定mRNAs 处理ncRNA。这项提案将重点放在对 核糖核酸酶，特别是在细胞质mRNA降解中的作用。

PROJECT SUMMARY All life forms must maintain a homeostatic gene expression program, which is achieved at many different steps in gene expression, including by the degradation of specific mRNAs and non-coding RNAs. Many evolutionarily conserved RNA processing enzymes mediate these key post-transcriptional events, with important roles for endo and exoribonucleases. A number of inherited diseases are caused by mutations in endo- or exoribonuclease genes, further underscoring their importance for human health. Eukaryotes use exonucleases for the degradation of most cellular mRNAs and for the 5' and 3' end processing of many different ncRNAs. In addition, endonucleases target specific mRNAs as an initiating step in mRNA degradation and other endonucleases process ncRNAs. This proposal will focus on the functional characterization of ribonucleases, especially functions in cytoplasmic mRNA degradation.