RNAse functions in post-transcriptional gene regulation

项目摘要 所有的生命形式都必须保持一个稳态的基因表达程序， 在基因表达的许多不同步骤中，包括通过降解特定的 mRNA和非编码RNA。许多进化上保守的RNA加工 酶介导这些关键的转录后事件，其中内切酶在转录后事件中起重要作用。 和核糖核酸外切酶。许多遗传性疾病是由内- 或核糖核酸外切酶基因，进一步强调了它们对人类健康的重要性。 真核生物使用外切核酸酶降解大多数细胞mRNA和5'端RNA。 和许多不同ncRNA的3'末端加工。此外，核酸内切酶靶向 特异性mRNA作为mRNA降解和其他内切核酸酶的起始步骤 加工ncRNA。本提案将侧重于 核糖核酸酶，特别是在细胞质mRNA降解中起作用。

PROJECT SUMMARY All life forms must maintain a homeostatic gene expression program, which is achieved at many different steps in gene expression, including by the degradation of specific mRNAs and non-coding RNAs. Many evolutionarily conserved RNA processing enzymes mediate these key post-transcriptional events, with important roles for endo and exoribonucleases. A number of inherited diseases are caused by mutations in endo- or exoribonuclease genes, further underscoring their importance for human health. Eukaryotes use exonucleases for the degradation of most cellular mRNAs and for the 5' and 3' end processing of many different ncRNAs. In addition, endonucleases target specific mRNAs as an initiating step in mRNA degradation and other endonucleases process ncRNAs. This proposal will focus on the functional characterization of ribonucleases, especially functions in cytoplasmic mRNA degradation.