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RNA decay and processing activities of the RNA exosome

RNA 外泌体的 RNA 降解和加工活性

基本信息

批准号：
9920152
负责人：
AMBRO VAN HOOF
金额：
$ 37.23万
依托单位：
UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-05-01 至 2022-04-30
项目状态：
已结题

项目摘要

Abstract The RNA exosome plays important roles in 3' end processing of a variety of ncRNAs and is also responsible for 3' to 5' degradation of both mRNAs and nc RNAs. By preferentially degrading aberrant mRNAs and ncRNAs it also maintains the overall fidelity of gene expression. Given the central role of the RNA exosome in a wide variety of RNA processing and degradation reactions, it is not surprising that it is required for cell viability. The long tern goal of our research is to achieve a greater understanding of how the RNA exosome carries out its diverse functions. The RNA exosome is a 10-subunit complex that adopts an overall donut-shaped structure. Nine of the subunits are catalytically inactive, and form the majority of the donut. The 10th subunit is associated with one opening of the donut and confers both 3' to 5' exoribonuclease and endoribonuclease activity on the RNA exosome complex. In the past 10 years much progress has been made in understanding the structure and biochemistry of the RNA exosome. Although isolated Rrp44 or the RNA exosome has nuclease activity in vitro, activity in vivo strictly requires over a dozen other proteins. Although it is clear that these RNA exosome cofactors control the activity and specificity of the RNA exosome, how these cofactors achieve this is essentially completely unknown. The overall goal of the proposed research is to resolve how the RNA exosome cofactors mediate its in vivo activities. We will focus on Ski7 and Rrp6, two of the three RNA exosome cofactors that are known to directly interact with the RNA exosome. Both genetic and biochemical data suggest that they are important in part because they mediate interaction with additional RNA exosome cofactors.

摘要

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The Mtr4 ratchet helix and arch domain both function to promote RNA unwinding.

DOI：
10.1093/nar/gku1208
发表时间：
2014-12-16
期刊：
Nucleic acids research
影响因子：
14.9
作者：
Taylor LL;Jackson RN;Rexhepaj M;King AK;Lott LK;van Hoof A;Johnson SJ
通讯作者：
Johnson SJ

The RNA exosome affects iron response and sensitivity to oxidative stress.

DOI：
10.1261/rna.043257.113
发表时间：
2014-07
期刊：
RNA (New York, N.Y.)
影响因子：
0
作者：
Tsanova B;Spatrick P;Jacobson A;van Hoof A
通讯作者：
van Hoof A

Yeast Nucleolin Nsr1 Impedes Replication and Elevates Genome Instability at an Actively Transcribed Guanine-Rich G4 DNA-Forming Sequence.

DOI：
10.1534/genetics.120.303736
发表时间：
2020-12
期刊：
Genetics
影响因子：
3.3
作者：
Singh S;Berroyer A;Kim M;Kim N
通讯作者：
Kim N

The RNA Exosome Channeling and Direct Access Conformations Have Distinct In Vivo Functions.

DOI：
10.1016/j.celrep.2016.08.059
发表时间：
2016-09-20
期刊：
Cell reports
影响因子：
8.8
作者：
Han J;van Hoof A
通讯作者：
van Hoof A

Interaction between the RNA-dependent ATPase and poly(A) polymerase subunits of the TRAMP complex is mediated by short peptides and important for snoRNA processing.

DOI：
10.1093/nar/gkv005
发表时间：
2015-02-18
期刊：
Nucleic acids research
影响因子：
14.9
作者：
Losh JS;King AK;Bakelar J;Taylor L;Loomis J;Rosenzweig JA;Johnson SJ;van Hoof A
通讯作者：
van Hoof A