Project 2: Neural Basis of Motor Pattern Control Loops

项目2：运动模式回路的神经基础 摘要 项目2的目标是了解运动模式回路如何将高级行为目标与 低水平的肌肉控制。在果蝇中，这种运动模式回路由以下种群组成： 下行神经元（DN），其整合中枢脑中的树突信号并投射其轴突 腹神经索（ventral nerve cord，VNC）我们将利用细胞类型特异性Split-Gal 4系来记录 并操纵DN中的活动，这些DN针对控制苍蝇腿和翅膀的VNC电路。我们将 将DN的光遗传学操作与行走和飞行行为的精细尺度分析联合收割机组合，以及 用全细胞电生理学测量行为动物的细胞内DN信号 录音.这些努力分为以下具体目标： 具体目标1：确定翅膀肌肉动作组是如何被招募到功能 通过下降控制。 具体目标2：确定腿部肌肉动作组如何被招募到功能性 通过下降控制。 具体目标3：表征脑中已识别的下行神经元的活动模式。 行为苍蝇使用在体内全细胞记录。

Project 2: The Neural Basis of Motor Pattern Loops Abstract The goal of Project 2 is to understand how motor pattern loops link high-level behavioral goals with low-level control of muscles. In the fly, such motor pattern loops are comprised of populations of descending neurons (DNs), which integrate dendritic signals in the central brain and project their axons to the ventral nerve cord (VNC). We will take advantage of cell-type specific Split-Gal4 lines to record and manipulate activity in DNs that target VNC circuits that control the fly legs and wings. We will combine optogenetic manipulation of DNs with fine-scale analysis of walking and flight behavior, and measure intracellular DN signals in behaving animals with whole whole-cell electrophysiology recordings. These efforts are divided into the following Specific Aims: Specific Aim 1: Determine how wing muscle action groups are recruited into functional actions by descending control. Specific Aim 2: Determine how leg muscle action groups are recruited into functional actions by descending control. Specific Aim 3: Characterize the activity patterns of identified descending neurons in behaving flies using in vivo whole-cell recordings.