Project 2: Neural Basis of Motor Pattern Control Loops

项目 2：运动模式控制环路的神经基础

• 批准号: 10202762
• 负责人: RICHARD S MANN
• 金额: $ 46.51万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-30 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10202762
• 关键词: Animals; Axon; Behavior; Behavioral; Brain; Cells; Cervical; Communication; Electrophysiology (science); Gait; Goals; Interneurons; Joints; Lead; Leg; Limb structure; Link; Locomotion; Lumbar Regions; Measures; Motor; Motor Neurons; Muscle; Nerve; Nervous system structure; Neuraxis; Neurons; Pattern; Periodicity; Play; Population; Running; Signal Transduction; Spinal Cord; System; Time; Walking; Whole-Cell Recordings; Wing; Work; Zaleplon; base; cell type; flexibility; fly; in vivo; kinematics; limb movement; optogenetics; programs; recruit; relating to nervous system; synergism

Project 2: The Neural Basis of Motor Pattern Loops Abstract The goal of Project 2 is to understand how motor pattern loops link high-level behavioral goals with low-level control of muscles. In the fly, such motor pattern loops are comprised of populations of descending neurons (DNs), which integrate dendritic signals in the central brain and project their axons to the ventral nerve cord (VNC). We will take advantage of cell-type specific Split-Gal4 lines to record and manipulate activity in DNs that target VNC circuits that control the fly legs and wings. We will combine optogenetic manipulation of DNs with fine-scale analysis of walking and flight behavior, and measure intracellular DN signals in behaving animals with whole whole-cell electrophysiology recordings. These efforts are divided into the following Specific Aims: Specific Aim 1: Determine how wing muscle action groups are recruited into functional actions by descending control. Specific Aim 2: Determine how leg muscle action groups are recruited into functional actions by descending control. Specific Aim 3: Characterize the activity patterns of identified descending neurons in behaving flies using in vivo whole-cell recordings.

项目2：马达模式回路的神经基础 摘要 项目2的目标是了解运动模式回路如何将高级行为目标与 对肌肉的低水平控制。在苍蝇中，这样的运动模式环由以下种群组成 下行神经元(Dns)，它整合中央大脑中的树突信号并投射轴突 至腹神经索(VNC)。我们将利用细胞类型的特定Split-Gal4线来记录 并操纵以控制苍蝇腿和翅膀的VNC电路为目标的DNS中的活动。我们会 将光遗传操作与行走和飞行行为的精细分析相结合，以及 全细胞电生理学测量行为动物细胞内糖尿病肾病信号 录音。这些努力分为以下具体目标： 具体目标1：确定翼肌动作组如何被招募到功能性 由降序控件执行的操作。 具体目标2：确定腿部肌肉动作组如何被招募到功能性 由降序控件执行的操作。 具体目标3：描述已确定的下行神经元的活动模式 使用活体全细胞记录的苍蝇行为。