Project 2: Neural Basis of Motor Pattern Control Loops

项目 2：运动模式控制环路的神经基础

• 批准号: 10202762
• 负责人: RICHARD S MANN
• 金额: $ 46.51万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-30 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10202762
• 关键词: Animals; Axon; Behavior; Behavioral; Brain; Cells; Cervical; Communication; Electrophysiology (science); Gait; Goals; Interneurons; Joints; Lead; Leg; Limb structure; Link; Locomotion; Lumbar Regions; Measures; Motor; Motor Neurons; Muscle; Nerve; Nervous system structure; Neuraxis; Neurons; Pattern; Periodicity; Play; Population; Running; Signal Transduction; Spinal Cord; System; Time; Walking; Whole-Cell Recordings; Wing; Work; Zaleplon; base; cell type; flexibility; fly; in vivo; kinematics; limb movement; optogenetics; programs; recruit; relating to nervous system; synergism

Project 2: The Neural Basis of Motor Pattern Loops Abstract The goal of Project 2 is to understand how motor pattern loops link high-level behavioral goals with low-level control of muscles. In the fly, such motor pattern loops are comprised of populations of descending neurons (DNs), which integrate dendritic signals in the central brain and project their axons to the ventral nerve cord (VNC). We will take advantage of cell-type specific Split-Gal4 lines to record and manipulate activity in DNs that target VNC circuits that control the fly legs and wings. We will combine optogenetic manipulation of DNs with fine-scale analysis of walking and flight behavior, and measure intracellular DN signals in behaving animals with whole whole-cell electrophysiology recordings. These efforts are divided into the following Specific Aims: Specific Aim 1: Determine how wing muscle action groups are recruited into functional actions by descending control. Specific Aim 2: Determine how leg muscle action groups are recruited into functional actions by descending control. Specific Aim 3: Characterize the activity patterns of identified descending neurons in behaving flies using in vivo whole-cell recordings.

项目2：运动模式循环的神经基础 抽象的 项目2的目标是了解电机模式循环如何将高级行为目标与 低水平的肌肉控制。在苍蝇中，这种运动图案环由 降序神经元（DNS），该神经元在中央大脑中整合树突状信号并投射轴突 到腹神经线（VNC）。我们将利用细胞类型特定的拆分式4线来记录 并操纵DNS的活性，该DNS针对控制飞腿和翅膀的VNC电路。我们将 将DNS的光学遗传操作与步行和飞行行为的精细分析，以及 测量具有全细胞电生理学的行为动物的细胞内DN信号 录音。这些努力分为以下具体目标： 特定目标1：确定如何将机翼肌肉作用组招募到功能 通过下降控制的动作。 特定目标2：确定如何将腿部肌肉动作组招募到功能 通过下降控制的动作。 特定目的3：表征已鉴定出的降降神经元的活动模式 使用体内全细胞记录的行为苍蝇。