Development and function of an adult locomotion circuit in Drosophila

果蝇成体运动回路的发育和功能

• 批准号: 8331731
• 负责人: RICHARD S MANN
• 金额: $ 3万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8331731
• 关键词: Address; Adult; Afferent Neurons; Aging; Animals; Axon; Behavior; Biological Assay; Biological Models; Candidate Disease Gene; Characteristics; Code; Complement; Complex; Date of birth; Development; Drosophila genus; Drosophila melanogaster; Embryo; Feedback; Functional disorder; Funding; Gene Expression Profiling; Genes; Genetic; Genetic Models; Genetic Screening; Goals; Hand; Learning; Leg; Locomotion; Longevity; Mind; Modeling; Molecular Biology; Monitor; Motor; Motor Neurons; Muscle; Muscle Contraction; Neuraxis; Neurodegenerative Disorders; Neurons; Organ; Paper; Play; Publishing; Role; Sensory; Specific qualifier value; Stem cells; System; Testing; Time; Vertebrates; Walking; Work; appendage; base; combinatorial; feeding; fly; follow-up; genetic manipulation; motor neuron development; nervous system development; neural circuit; neuromuscular; neuromuscular system; neuronal cell body; novel; public health relevance; sensory feedback; sensory system; tool; transcription factor

DESCRIPTION (provided by applicant): Animal locomotion requires the coordinated firing of motor neurons that trigger the contraction of muscles in the appendages, such as the legs. In addition, coordination requires feedback from sensory neurons located in the appendages. To understand how functional motor neuron circuits form, this project will investigate the development and function of the motor circuit used by the adult fruit fly for walking. The combinatorial transcription factor code required to give each motor neuron its particular identity will be deciphered using both novel genetic and molecular biology approaches. Once identified, the consequences on walking of perturbing small numbers of motor neurons will be analyzed. Finally, the role of sensory feedback from mechanosensory organs in the legs will be characterized. Together, these studies will lay the ground work for a new model genetic system for studying motor circuits in a genetically tractable animal that, unlike its larval counterpart, uses appendages for locomotion. PUBLIC HEALTH RELEVANCE: Animal locomotion requires the coordinated firing of motor neurons that trigger the contraction of muscles in the appendages, such as the legs. This project will investigate the development and function of the motor circuit used by the adult fruit fly for walking. The use of this model system will help to decipher the genes and neurons required for coordinated locomotion and, therefore, the eventual treatment of motor neuron-related dysfunction resulting from neurodegenerative diseases and aging.

描述（由申请人提供）：动物运动需要运动神经元的协调发射，从而触发附肢（例如腿部）的肌肉收缩。此外，协调需要来自位于附肢的感觉神经元的反馈。为了了解功能性运动神经元回路是如何形成的，该项目将研究成年果蝇用于行走的运动回路的发育和功能。赋予每个运动神经元其特定身份所需的组合转录因子代码将使用新颖的遗传和分子生​​物学方法来破译。一旦确定，将分析干扰少量运动神经元对行走的影响。最后，将描述腿部机械感觉器官的感觉反馈的作用。总之，这些研究将为研究遗传易驯化动物的运动回路的新模型遗传系统奠定基础，与幼虫不同，这种动物使用附肢进行运动。 公共健康相关性：动物的运动需要运动神经元的协调发射，从而触发四肢（例如腿部）的肌肉收缩。该项目将研究成年果蝇用于行走的运动电路的发育和功能。该模型系统的使用将有助于破译协调运动所需的基因和神经元，从而最终治疗由神经退行性疾病和衰老引起的运动神经元相关功能障碍。