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Development and function of an adult locomotion circuit in Drosophila

果蝇成体运动回路的发育和功能

基本信息

批准号：
10361422
负责人：
RICHARD S MANN
金额：
$ 46.15万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-04-01 至 2025-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10361422
关键词：
ATAC-seq Adult Affect Afferent Neurons Architecture Area Astrocytes Axon Binding Cells Cellular Morphology Chest Chromatin Code Complex Data Dendrites Development Drosophila genus Drosophila melanogaster Embryonic Development Equilibrium Family Family member Funding Gene Expression Gene Family Genes Genetic Goals Image Individual Instruction Interneurons Knowledge Larva Leg Locomotion Logic Maps Methods Mitotic Modeling Molecular Molecular Genetics Molecular Profiling Morphology Motor Motor Neurons Movement Muscle Muscle Fibers Names Neuraxis Neuroglia Neurons Neuropil Play Pupa Recording of previous events Regulator Genes Resolution Role Series Specific qualifier value Specificity Stereotyping Synapses System Testing Time Tissues Walking appendage combinatorial experience fly genome-wide in vivo insight member motor control motor neuron development neuroblast programs relating to nervous system synaptogenesis tool transcription factor transcriptome transcriptome sequencing whole genome

项目摘要

PROJECT SUMMARY The morphological diversity among neurons is enormous and critical to their function, yet many gaps in our knowledge remain concerning how this diversity is genetically encoded. As with vertebrate motor neurons, the motor neurons of Drosophila melanogaster establish highly specific synapses on the correct muscle fibers in the appendages and also elaborate highly stereotyped dendritic arbors in the central nervous system (CNS). In the previous funding period, we characterized the individual progeny and transcription factor codes that govern neuronal identities for one of the largest neuroblast lineages that give rise to motor neurons and neuropil glia. We further described the role of Ig domain family members in establishing certain aspects of motor neuron identities. Live imaging provided unprecedented access to the relevant stages of motor neuron maturation during pupal stages. In the coming funding period we will extend these findings to understand how transcription factors in post- mitotic neurons regulate their target genes. We will carry out a series of whole genome and single cell studies to obtain this information on a genome-wide scale. Finally, we will analyze additional members of the Ig domain superfamily to assess what other roles, besides axon targeting, they play in motor neuron morphology during development.

项目摘要神经元之间的形态多样性是巨大的，对它们的功能至关重要，但我们的研究中存在许多空白。关于这种多样性是如何遗传编码的知识仍然存在。与脊椎动物的运动神经元一样，果蝇的运动神经元在正确的肌肉纤维上建立高度特异性的突触，在中枢神经系统（CNS）中，它的附属物和复杂的高度定型的树突状乔木。在在前一个资助期，我们描述了个体后代和转录因子编码，最大的神经母细胞谱系之一，产生运动神经元和神经胶质细胞的神经元身份。我们进一步描述了IG结构域家族成员在建立运动神经元某些方面的作用，身份实时成像为运动神经元成熟的相关阶段提供了前所未有的途径在蛹期。在接下来的资助期内，我们将扩展这些发现，以了解转录因子在转录后有丝分裂神经元调节它们的靶基因。我们将开展一系列的全基因组和单细胞研究来获取全基因组范围内的信息。最后，我们将分析IG的其他成员结构域超家族，以评估除了轴突靶向之外，它们在运动神经元形态学中发挥的其他作用在发展过程中。