The olfactory basis of locating nectar sugar sources in Aedes aegypti mosquitoes

埃及伊蚊定位花蜜糖源的嗅觉基础

基本信息

  • 批准号:
    10207040
  • 负责人:
  • 金额:
    $ 63.76万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2021
  • 资助国家:
    美国
  • 起止时间:
    2021-03-05 至 2026-02-28
  • 项目状态:
    未结题

项目摘要

The objective of the proposed R01 project is to build on our recent findings that attractive odors from sources of nectar activate conserved olfactory channels in mosquitoes, and the odorant ratios within the scent, along with the inhibition in the antennal lobe (AL), are critical for Aedes aegypti behavior. Feeding on fruits and flowers is a vital behavior for mosquitoes, and mosquito attraction to these sources of nectar is mediated by the ratio of key odorants in the bouquet. Bait-and-kill traps that use fruit syrups have effectively controlled local mosquito populations, but variation in the fruit odor can strongly impact its attractiveness. We still have not identified the odor constituents and ratios that are attractive in the nectar odors – enabling the development of synthetic lures –, nor how this information is detected and processed by the mosquito's olfactory system. Nectar-feeding by female mosquitoes increases their life-span and decreases the gonotrophic cycle, thereby increasing their vectorial capacity, and for adult males, it is the only source of nutrients. Our recent work allowed us to identify the odor constituents that mediate nectar-feeding behaviors in Ae. aegypti and understand how the odor is processed in the brain. Now in this application, we propose to use a combination of behavioral assays, chemical methods combined with calcium imaging in tethered flying mosquitoes, and genetic approaches to study the olfactory basis of nectar-seeking behaviors. Aim 1 will allow us to identify attractive odorants in the scents of diverse plant nectar sources and use heterologous expression systems to de-orphan the cognate odorant receptors (Ors). We also take advantage of the Q-system for genetically characterizing three Ors (Or15, Or49, and Or2) that are essential for representing the proper ratios in nectar odors. In Aim 2, we leverage our existing and new GCaMP expression lines to examine how the ratios of these key odorants are processed in the AL, and how GABAergic inhibition shapes these responses. In Aim 3, we will use our identified odor lures, and artificial lures that vary in their natural ratios of key odorants, to determine their efficacy in bait-and-kill systems. Together, these experiments will test the working hypothesis that nectar odors and their specific odorant ratios activate conserved olfactory channels to be processed similarly by AL circuits to mediate feeding behaviors. While there has been an extensive study of mosquito attraction to blood hosts, we know comparatively less about nectar feeding. Our experiments will identify new odors that can be immediately deployed as attractant lures. Additionally, GABAergic systems are involved in diverse physiological processes in insect vectors, including olfaction, immune response, and arbovirus replication, as well as being potent targets for insecticides. Generating mutants that target the mosquito's olfactory responses or GABAergic pathways could provide additional insight into these diverse processes. Sugar feeding plays an essential role in the vectorial capacity of mosquitoes and the spread of diseases that afflict over a billion people annually. Therefore, unraveling the neural bases of nectar-feeding will enable new gene-targets and tools for their control.
建议的R 01项目的目标是建立在我们最近的研究结果, 花蜜激活蚊子保守的嗅觉通道,气味中的气味比例,沿着 触角叶(AL)的抑制作用对埃及伊蚊的行为至关重要。以水果和花朵为食, 这是蚊子的一种重要行为,蚊子对这些花蜜来源的吸引力是由以下比例介导的: 香味中的关键气味。使用水果糖浆的诱捕器有效地控制了当地的蚊子 但水果气味的变化会强烈影响其吸引力。我们还没有确定 花蜜气味中吸引人的气味成分和比例-使合成诱饵的开发成为可能 也不知道蚊子的嗅觉系统如何检测和处理这些信息。 雌性蚊子以花蜜为食可以延长它们的寿命,减少生殖营养循环, 增加它们的媒介能力,对于成年雄性来说,它是唯一的营养来源。我们最近的工作 我们确定的气味成分,介导花蜜喂养行为的Ae。埃及和了解如何 气味是在大脑中处理的。现在在这个应用中,我们建议使用行为分析的组合, 化学方法结合钙成像在系留飞行蚊子,和遗传方法, 研究寻找花蜜行为的嗅觉基础。目标1将使我们能够识别有吸引力的气味, 不同植物花蜜来源的气味,并使用异源表达系统去孤儿同源 气味受体(Ors)。我们还利用Q系统来遗传地表征三个Or(Or 15, Or 49和Or 2),这是必不可少的代表正确的比例花蜜气味。在目标2中,我们利用 现有的和新的GCaMP表达线,以检查这些关键气味剂的比例是如何处理的, AL,以及GABA能抑制如何塑造这些反应。在目标3中,我们将使用我们确定的气味诱饵, 人工诱饵,其关键气味剂的天然比例不同,以确定其在诱饵和杀伤系统的功效。 总之,这些实验将测试工作假设,花蜜气味和它们的特定气味比例 激活保守的嗅觉通道,类似地由AL回路处理,以介导进食行为。 虽然对蚊子对血液宿主的吸引力进行了广泛的研究,但我们知道的相对较少 关于花蜜喂养。我们的实验将确定新的气味,可以立即部署作为引诱剂 诱饵此外,GABA能系统参与昆虫载体中的多种生理过程, 包括嗅觉、免疫反应和虫媒病毒复制,以及作为杀虫剂的有效靶标。 产生针对蚊子嗅觉反应或GABA能通路的突变体可以提供 对这些不同过程的进一步了解。糖饲料在介导能力中起着至关重要的作用, 蚊子和疾病的传播每年折磨着超过10亿人。因此,解开 花蜜喂养的神经基础将使新的基因目标和工具,他们的控制。

项目成果

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Omar Sultan Akbari其他文献

Omar Sultan Akbari的其他文献

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{{ truncateString('Omar Sultan Akbari', 18)}}的其他基金

Development of precision genome editing tools in Ae. albopictus for functional genetics and mosquito control technologies
开发 Ae 中的精确基因组编辑工具。
  • 批准号:
    10362718
  • 财政年份:
    2021
  • 资助金额:
    $ 63.76万
  • 项目类别:
The olfactory basis of locating nectar sugar sources in Aedes aegypti mosquitoes
埃及伊蚊定位花蜜糖源的嗅觉基础
  • 批准号:
    10802906
  • 财政年份:
    2021
  • 资助金额:
    $ 63.76万
  • 项目类别:
The olfactory basis of locating nectar sugar sources in Aedes aegypti mosquitoes
埃及伊蚊定位花蜜糖源的嗅觉基础
  • 批准号:
    10366069
  • 财政年份:
    2021
  • 资助金额:
    $ 63.76万
  • 项目类别:
The olfactory basis of locating nectar sugar sources in Aedes aegypti mosquitoes
埃及伊蚊定位花蜜糖源的嗅觉基础
  • 批准号:
    10582687
  • 财政年份:
    2021
  • 资助金额:
    $ 63.76万
  • 项目类别:
Precision guided SIT for the control of vector-borne disease
精准引导昆虫不育技术用于控制媒介传播疾病
  • 批准号:
    10326334
  • 财政年份:
    2020
  • 资助金额:
    $ 63.76万
  • 项目类别:
Precision guided SIT for the control of vector-borne disease
精准引导昆虫不育技术用于控制媒介传播疾病
  • 批准号:
    10533815
  • 财政年份:
    2020
  • 资助金额:
    $ 63.76万
  • 项目类别:
Precision guided SIT for the control of vector-borne disease
精准引导昆虫不育技术用于控制媒介传播疾病
  • 批准号:
    10087886
  • 财政年份:
    2020
  • 资助金额:
    $ 63.76万
  • 项目类别:
Development of synthetic gene drives using small molecules
使用小分子开发合成基因驱动器
  • 批准号:
    10470220
  • 财政年份:
    2019
  • 资助金额:
    $ 63.76万
  • 项目类别:
Development of synthetic gene drives using small molecules
使用小分子开发合成基因驱动器
  • 批准号:
    10254421
  • 财政年份:
    2019
  • 资助金额:
    $ 63.76万
  • 项目类别:
Developing reciprocal chromosomal translocations for wild population replacement in an important vector of human disease.
开发相互染色体易位以替代人类疾病的重要媒介中的野生种群。
  • 批准号:
    9243803
  • 财政年份:
    2016
  • 资助金额:
    $ 63.76万
  • 项目类别:

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确定沃尔巴克氏体对埃及伊蚊的抗病毒作用
  • 批准号:
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  • 财政年份:
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  • 批准号:
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合作研究:埃及伊蚊的嗅觉学习和神经调节
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博士论文研究:埃及伊蚊的系统发育地理学调查
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