Iron and Heme Core

项目摘要 铁和血红素核心，通过NIDDK赞助的铁和血红素疾病中心（CIHD）创建， 提供金属（主要是铁）、卟啉、卟啉前体和血红素的分析。核还 测量血红素生物合成途径中酶的活性。分析和绝对定量 细胞、组织、全血、尿液和粪便中的这些途径中间体或酶活性可用于 全国实验室这个核心是许多实验室的核心，这些实验室测量这些酶的活性，化合物和 金属在不同的实验系统。对这一核心的需要是由以下事实证明的： 在过去四年中，通过充电，为20家研究机构的34个不同实验室提供服务 系统在此期间，该核心已分析了4000多个样本。CIHD研究者提供 优先享受这些服务。核心工作人员也可以主持培训班， 各级研究人员在实验设计中最大限度地利用核心既定方法。我们 在CIHD网站上分享我们的协议，在我们的领域内提供一致性，验证和质量控制， research.铁和血红素核心提供的独特服务已成为国家资源。

PROJECT SUMMARY The Iron and Heme Core, created through the NIDDK sponsored Center for Iron and Heme Disorders (CIHD), provides analysis of metals (primarily iron), porphyrins, porphyrin precursors and heme. The core also measures activity of the enzymes in the heme biosynthesis pathway. Analysis and absolute quantitation of these pathway intermediates or enzyme activities in cells, tissue, whole blood, urine and feces is available to laboratories nationally. This core is central for many labs that measure these enzyme activities, compounds & metals in a diverse range of experimental systems. The need for this core is borne out by the fact that it has served more than 34 different laboratories from 20 research institutions in the past four years via its recharge system. This core has assayed more than 4000 samples during this time. CIHD investigators are provided priority access to these services. Core staff members are also available to host training sessions to assist researchers at all levels in experimental design to leverage the most from Core established methods. We share our protocols on the CIHD website providing consistency, validation and quality control within our field of research. The unique services the Iron and Heme Core offers has become a national resource.