Iron and Heme Core

项目总结 铁和血红素核心，由NIDDK赞助的铁和血红素疾病中心(CIHD)创建， 提供对金属(主要是铁)、卟啉、卟啉前体和血红素的分析。核心还包括 测量血红素生物合成途径中的酶的活性。的分析和绝对量化 这些途径的中间产物或酶在细胞、组织、全血、尿液和粪便中的活性可用于 全国实验室。这一核心是许多实验室测量这些酶活性、化合物和 金属在不同的实验系统中。对这个核心的需求从以下事实得到了证明：它已经 在过去的四年里，它通过充值为来自20个研究机构的34个不同的实验室提供了服务 系统。在此期间，该岩芯已经检测了4000多个样品。提供了CIHD调查人员 优先使用这些服务。核心工作人员也可以主持培训课程，以帮助 各级研究人员在实验设计中最大限度地利用Core既定的方法。我们 在CIHD网站上共享我们的协议，在我们的领域内提供一致性、验证和质量控制 研究。铁和血红素核心提供的独特服务已成为国家资源。

PROJECT SUMMARY The Iron and Heme Core, created through the NIDDK sponsored Center for Iron and Heme Disorders (CIHD), provides analysis of metals (primarily iron), porphyrins, porphyrin precursors and heme. The core also measures activity of the enzymes in the heme biosynthesis pathway. Analysis and absolute quantitation of these pathway intermediates or enzyme activities in cells, tissue, whole blood, urine and feces is available to laboratories nationally. This core is central for many labs that measure these enzyme activities, compounds & metals in a diverse range of experimental systems. The need for this core is borne out by the fact that it has served more than 34 different laboratories from 20 research institutions in the past four years via its recharge system. This core has assayed more than 4000 samples during this time. CIHD investigators are provided priority access to these services. Core staff members are also available to host training sessions to assist researchers at all levels in experimental design to leverage the most from Core established methods. We share our protocols on the CIHD website providing consistency, validation and quality control within our field of research. The unique services the Iron and Heme Core offers has become a national resource.