Iron and Heme Core (Core B)

项目总结 -- 铁和血红素疾病中心(CIHD)的铁和血红素核心将提供金属分析， 卟啉、卟啉前体和亚铁血红素。核心将测量八种酶的酶活性 血红素生物合成途径。这一核心在所提供的服务中是独一无二的。分析与绝对 对细胞、组织、全血、尿液和粪便中许多此类化合物的定量将可用于 全国实验室。Core还将提供一系列试剂，包括用于表达 血红素合成中的蛋白质，对这些蛋白质起反应的抗体，以及可以 用于提纯卟啉途径中间体，以用于其他实验系统。核心员工将主持 举办培训课程，协助私人投资主任、博士后研究员和研究生执行上述服务， 包括旨在进行数据分析的会议。将向CIHD调查人员提供优先访问这些 通过敬业的核心员工提供服务。成为国家资源是铁和血红素核心的目标 用于在各种实验系统中测量来自细菌的这些酶/化合物 对人类来说。

PROJECT SUMMARY   The Iron and Heme Core of the Center for Iron and Heme Disorders (CIHD) will provide analysis of metals, porphyrins, porphyrin precursors and heme. The Core will measure enzymatic activity of the eight enzymes of the heme biosynthesis pathway. This Core is unique in the services offered. Analysis and absolute quantitation of many of these compounds in cells, tissue, whole blood, urine and feces will become available to laboratories nationally. The Core will also provide a series of reagents, including plasmids for expression of proteins in heme synthesis, antibodies reactive to these proteins, and unique strains of bacteria that can be used to purify porphyrin pathway intermediates for use in other experimental systems. Core staff will host training sessions to assist PIs, post-doctoral fellows and graduate students in performing the services above, including sessions aimed at data analysis. CIHD investigators will be provided priority access to these services through dedicated Core staff. It is the goal of the Iron and Heme Core to become a national resource for the measurement of these enzymes/compounds in a diverse range of experimental systems from bacteria to human.