Environment Exposure & Neurotoxicology Core

环境暴露与神经毒理学核心(核心C) 董事，C Sioutas，联席董事：JC Chen和H Forman 摘要 由C.Sioutas执导的环境暴露和神经毒理学核心C包括三个支持 通过提供特定于住所的曝光，为拟议的科学项目服务的分核心 种群估计，动物模型吸入暴露实验的标准化，以及 交通相关空气污染(TRAP)的神经毒性反应分析。子核C1，环境数据 支持(CoDir：JC Chen)为项目1和2提供个人级别的、特定于位置的长期- 术语陷阱曝光，使用最先进的时空建模方法协调两个 以人类人口为基础的队列。分核心C2，Trap-NPM(Dir：C.Sioutas)用品收藏 实验项目3和4具有良好的诱捕器吸入暴露氛围。陷阱是 在受交通废气影响的城市地点附近进行采样。亚核C3，脑细胞对TRAP-PM的反应 (I)使用THP-1细胞测试个别批次收集的NPM的细胞因子表达；以及 (Ii)在项目3和项目4中，对暴露于体内NPM的脑蛋白变化进行蛋白质印迹分析。

Environmental Exposures and Neurotoxicology Core (Core C) Director, C Sioutas, Co-Directors: JC Chen and H Forman Abstract The Environmental Exposures and Neurotoxicology Core C, directed by C. Sioutas, includes three supporting subcores to serve the proposed scientific projects, through the provision of the residence-specific exposure estimates in populations, standardization of the inhalation exposure experiments in animal models, and analyses of the neurotoxic responses to traffic-related air pollution (TRAP). SubCore C1, Environmental Data Support (coDir: JC Chen) provides Projects 1 and 2 with individual-level, location-specific estimates of long- term TRAP exposure, using state-of-the-art spatio-temporal modeling approaches harmonized across two human population-based cohorts. Subcore C2, Collection of TRAP-nPM (Dir: C.Sioutas) supplies experimental Projects 3 and 4 with well-characterized TRAP inhalation exposure atmospheres. TRAP are sampled near urban sites impacted by traffic emissions. SubCore C3, Brain Cell Responses to TRAP-PM (coDir: H. Forman) (i) tests individual batches of collected nPM using THP-1 cells for cytokine expression; and (ii) Western blot assay of brain protein changes to in vivo nPM exposure for Projects 3 & 4.