Environment Exposure & Neurotoxicology Core

环境暴露

• 批准号: 10456751
• 负责人: Constantinos Sioutas
• 金额: $ 23.23万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10456751
• 关键词: Aerosols; Aging; Air; Air Pollution; Alzheimer' s disease risk; Ammonium; Animal Model; Animals; Atmosphere; Biological Assay; Brain; CD36 gene; Carbon; Cell Line; Cells; Chemicals; Chromatography; Collection; Complement 5a; Coupled; Data; Environment; Environmental Exposure; Freezing; Funding; Geography; Heterogeneity; Human; IL6 gene; Individual; Infrastructure; Inhalation; Inhalation Exposure; Interleukin-10; Ions; Liquid substance; Location; Los Angeles; Magnetism; Mass Spectrum Analysis; Memory; Modeling; Monitor; Multi-Ethnic Study of Atherosclerosis; Mus; Nitrates; Ozone; Particulate; Particulate Matter; Plasma; Population; Population Heterogeneity; Population Study; Proteins; Protocols documentation; Publishing; Sampling; Site; Standardization; Sulfate; Suspensions; TLR4 gene; TNF gene; Technology; Testing; Trace Elements; Trace metal; Twin Studies; United States National Institutes of Health; Vietnam; Washington; Water; Western Blotting; Women' s Health; Work; ambient air pollution; aqueous; base; brain cell; cohort; cytokine; data resource; design; epidemiology study; experimental study; in vivo; innovation; nanosized; neurotoxic; neurotoxicology; population based; residence; response; spatiotemporal; tool; traffic-related air pollution; ultrafine particle

Environmental Exposures and Neurotoxicology Core (Core C) Director, C Sioutas, Co-Directors: JC Chen and H Forman Abstract The Environmental Exposures and Neurotoxicology Core C, directed by C. Sioutas, includes three supporting subcores to serve the proposed scientific projects, through the provision of the residence-specific exposure estimates in populations, standardization of the inhalation exposure experiments in animal models, and analyses of the neurotoxic responses to traffic-related air pollution (TRAP). SubCore C1, Environmental Data Support (coDir: JC Chen) provides Projects 1 and 2 with individual-level, location-specific estimates of long- term TRAP exposure, using state-of-the-art spatio-temporal modeling approaches harmonized across two human population-based cohorts. Subcore C2, Collection of TRAP-nPM (Dir: C.Sioutas) supplies experimental Projects 3 and 4 with well-characterized TRAP inhalation exposure atmospheres. TRAP are sampled near urban sites impacted by traffic emissions. SubCore C3, Brain Cell Responses to TRAP-PM (coDir: H. Forman) (i) tests individual batches of collected nPM using THP-1 cells for cytokine expression; and (ii) Western blot assay of brain protein changes to in vivo nPM exposure for Projects 3 & 4.

环境暴露和神经毒理学核心（核心C） 导演，C Sioutas，联合导演：JC Chen和H Forman 摘要 环境暴露和神经毒理学核心C，由C。Sioutas，包括三个支持 通过提供特定驻地的接触， 人群中的估计值，动物模型中吸入暴露实验的标准化，以及 交通相关空气污染（traffic related air pollution，TRAP）。SubCore C1，环境数据 Support（coDir：JC Chen）为项目1和项目2提供了个人层面的、特定于地点的长期 长期陷阱暴露，使用最先进的时空建模方法，在两个 基于人类群体的队列。子核心C2，TRAP-nPM集合（导演：C.Sioutas）用品 实验项目3和4具有良好表征的TRAP吸入暴露气氛。阱 在受交通排放影响的城市附近采样。亚核心C3，脑细胞对TRAP-PM的反应 （coDir：H.（i）使用THP-1细胞测试单个批次的收集的nPM的细胞因子表达;和 (ii)对于项目3和4，脑蛋白质对体内nPM暴露的改变的蛋白质印迹测定。