Metabolism and Translational Science Core

摘要 佛罗里达大学(UF)老年人独立中心(OAIC)支持整体 主题为“促进流动性和独立性”。代谢与翻译科学核心(RC2)，in 与我们的其他UF OAIC核心合作，支持临床前、人类 干预性的或观察性的临床研究。通过测量一组选定的生物标志物，我们可以确定 有针对性的干预措施如何影响老龄化速度以及丧失机动性和独立性。这个核心 从而为研究教育核心(REC)学者和先导研究人员提供支持。 衰老和疾病的特征是各种生理和代谢过程的渐进性恶化。 这与蛋白质、RNA和DNA的功能或含量改变有关，它们为 监控老化。多条途径和结构域与衰老有关，例如基因组不稳定 (包括端粒磨损、突变和缺失)；表观遗传学改变；蛋白稳定性丧失(包括 自噬功能障碍)；放松营养感知；线粒体(MT)功能障碍；炎症和 细胞衰老；干细胞衰竭；昼夜节律紊乱；以及功能失调的烟酰胺 腺嘌呤二核苷酸(NAD)动态平衡。蛋白质、RNA和DNA生物标记物的具体分析 这一核心将提供与已知的调节衰老和焦点的主要生物和代谢途径有关的 关于：(I)线粒体功能；(Ii)炎症和衰老；(Iii)自噬；(Iv)生物钟生物学；以及 (V)NAD动态平衡。我们使用创新的分析工具和标准的高通量分析 确定衰老的基本生物学机制。新陈代谢与翻译科学的核心 (RC2)支持关于特定蛋白质、RNA和DNA生物标记物的知识，如 以及对分离的线粒体和白细胞(WBC)代谢的测量，对于 了解健康老龄化的轨迹和行动不便的潜在生物学原因。 我们将支持提取蛋白质、RNA和DNA；分析生物标志物；分离细胞(WBC) 和细胞器(线粒体)；以及线粒体功能的评估。RC2将为全国各地的调查人员提供 具有既定方法的OAIC核心和REC学者；科学数据；基础设施；高度合格 人员；以及咨询和协作方面的专门知识。我们在完成REC研究方面有着丰富的历史 UF的学者和高级调查人员以及全国各地的科学家。 RC2追求以下目标： 目的1：支持蛋白质、RNA和DNA的分离和分析衰老的特定生物标志物。 目的2：支持线粒体呼吸、线粒体酶活性和NAD辅酶的分析。 目标3：促进和提供分析和样品保存方面的咨询，并协同合作 与伊斯兰会议组织的其他核心一起追求伊斯兰会议组织促进机动性和独立性的共同主题。

Summary The University of Florida (UF) Older Americans Independence Center (OAIC) supports the overall theme of “promotion of mobility and independence.” The Metabolism and Translational Science Core (RC2), in collaboration with our other UF OAIC cores, supports biochemical analyses for preclinical, human interventional, or observational clinical studies. By measuring a selected set of biomarkers, we can determine how targeted interventions influence the rate of aging, as well as loss of mobility and independence. This core thereby provides the support for the Research Education Core (REC) Scholars and pilot study investigators. Aging and disease feature progressive deterioration of various physiological and metabolic processes. This is associated with altered functions or contents of protein, RNA, and DNA, which provide biomarkers to monitor aging. Multiple pathways and domains have been associated with aging, such as genomic instability (including telomere attrition, mutations, and deletions); epigenetic alterations; loss of proteostasis (including dysfunctional autophagy); deregulated nutrient sensing; mitochondrial (Mt) dysfunction; inflammation and cellular senescence; stem cell exhaustion, disrupted circadian clock rhythms; and dysfunctional nicotinamide adenine dinucleotide (NAD+) homeostasis. The specific analyses of protein, RNA, and DNA biomarkers that this core will provide are related to major biological and metabolic pathways known to regulate aging and focus on: (i) Mt function; (ii) inflammation and senescence; (iii) autophagy; (iv) circadian clock biology; and (v) NAD+ homeostasis. We use innovative analytical tools and standard high-throughput analysis to determine the fundamental biological mechanisms of aging. The Metabolism and Translational Science Core (RC2) supports the overarching hypothesis that knowledge of specific protein, RNA, and DNA biomarkers, as well as measurements of metabolism of isolated mitochondria and white blood cells (WBCs), are critical for understanding the trajectory of healthy aging and the underlying biological causes of mobility loss. We will support extraction of proteins, RNA, and DNA; analysis of biomarkers; isolation of cells (WBCs) and organelles (mitochondria); and assessments of Mt function. RC2 will provide investigators across the OAIC Cores and REC Scholars with established methodologies; scientific data; infrastructure; highly qualified personnel; and consultative and collaborative expertise. We have a rich history of completing studies for REC Scholars and senior investigators at UF, as well as scientists around the country. RC2 pursues the following aims: Aim 1: To support protein, RNA, and DNA isolation and analysis of specific biomarkers of aging. Aim 2: To support analysis of Mt respiration, Mt enzyme activities, and NAD coenzymes. Aim 3: To facilitate and provide consultation on analyses and sample storage, and collaborate synergistically with the other OAIC cores to pursue the common OAIC theme of promotion of mobility and independence.