Metabolism and Translational Science Core

代谢与转化科学核心

• 批准号: 10631863
• 负责人: CHRISTIAAN LEEUWENBURGH
• 金额: $ 24.52万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10631863
• 关键词: Aging; American; Ancillary Study; Autophagocytosis; Biochemical; Biological; Biological Markers; Biology; Blood; Blood capillaries; Brain; Cell Aging; Cell Separation; Cell physiology; Cells; Circadian Dysregulation; Clinical Research; Clinical Sciences; Coenzymes; Collaborations; Complementary DNA; Complex; Consultations; Country; DNA; DNA copy number; Data; Deterioration; Disease; Elderly; Electron Transport; Enzyme-Linked Immunosorbent Assay; Epigenetic Process; Fatty acid glycerol esters; Florida; Freezing; Future; Gene Expression Profiling; Genomic Instability; Genomics; Geroscience; Goals; Homeostasis; Human; Human Resources; Immunoassay; Impairment; Inflammation; Infrastructure; Intervention; Knowledge; Laboratories; Leukocytes; Longevity; Measures; Metabolic Pathway; Metabolism; Methodology; Mitochondria; Mitochondrial DNA; Molecular; Monitor; Muscle; Mutation; NADH; NADP; Nicotinamide adenine dinucleotide; Nuclear; Organelles; Pathway interactions; Pattern; Physical Function; Physiological Processes; Pilot Projects; Plasma; Principal Investigator; Protein Analysis; Proteins; Proteomics; Qualifying; RNA; Reaction; Reader; Recording of previous events; Research; Research Personnel; Research Support; Resolution; Resources; Respiration; Respiratory physiology; Sampling; Scientist; Sensory; Serum; Services; Spirometry; Techniques; Technology; Time; Tissue Sample; Tissues; Touch sensation; Translational Research; Universities; Vertebral column; Western Blotting; Whole Blood; age related; analytical tool; austin; biobank; biomarker selection; circadian pacemaker; cognitive function; design; detection of nutrient; detection platform; education research; enzyme activity; exhaustion; healthy aging; high standard; high throughput analysis; innovation; interest; metabolomics; mitochondrial dysfunction; nanoimmunoassay; novel; pre-clinical; proteostasis; research study; senescence; specific biomarkers; stem cells; telomere; transcriptomics

Summary The University of Florida (UF) Older Americans Independence Center (OAIC) supports the overall theme of “promotion of mobility and independence.” The Metabolism and Translational Science Core (RC2), in collaboration with our other UF OAIC cores, supports biochemical analyses for preclinical, human interventional, or observational clinical studies. By measuring a selected set of biomarkers, we can determine how targeted interventions influence the rate of aging, as well as loss of mobility and independence. This core thereby provides the support for the Research Education Core (REC) Scholars and pilot study investigators. Aging and disease feature progressive deterioration of various physiological and metabolic processes. This is associated with altered functions or contents of protein, RNA, and DNA, which provide biomarkers to monitor aging. Multiple pathways and domains have been associated with aging, such as genomic instability (including telomere attrition, mutations, and deletions); epigenetic alterations; loss of proteostasis (including dysfunctional autophagy); deregulated nutrient sensing; mitochondrial (Mt) dysfunction; inflammation and cellular senescence; stem cell exhaustion, disrupted circadian clock rhythms; and dysfunctional nicotinamide adenine dinucleotide (NAD+) homeostasis. The specific analyses of protein, RNA, and DNA biomarkers that this core will provide are related to major biological and metabolic pathways known to regulate aging and focus on: (i) Mt function; (ii) inflammation and senescence; (iii) autophagy; (iv) circadian clock biology; and (v) NAD+ homeostasis. We use innovative analytical tools and standard high-throughput analysis to determine the fundamental biological mechanisms of aging. The Metabolism and Translational Science Core (RC2) supports the overarching hypothesis that knowledge of specific protein, RNA, and DNA biomarkers, as well as measurements of metabolism of isolated mitochondria and white blood cells (WBCs), are critical for understanding the trajectory of healthy aging and the underlying biological causes of mobility loss. We will support extraction of proteins, RNA, and DNA; analysis of biomarkers; isolation of cells (WBCs) and organelles (mitochondria); and assessments of Mt function. RC2 will provide investigators across the OAIC Cores and REC Scholars with established methodologies; scientific data; infrastructure; highly qualified personnel; and consultative and collaborative expertise. We have a rich history of completing studies for REC Scholars and senior investigators at UF, as well as scientists around the country. RC2 pursues the following aims: Aim 1: To support protein, RNA, and DNA isolation and analysis of specific biomarkers of aging. Aim 2: To support analysis of Mt respiration, Mt enzyme activities, and NAD coenzymes. Aim 3: To facilitate and provide consultation on analyses and sample storage, and collaborate synergistically with the other OAIC cores to pursue the common OAIC theme of promotion of mobility and independence.

总结 佛罗里达大学（UF）老年美国人独立中心（OAIC）支持整体 主题是“促进流动性和独立性”。代谢和转化科学核心（RC 2） 与我们的其他UF OAIC核心合作，支持临床前，人类 干预性或观察性临床研究。通过测量一组选定的生物标志物，我们可以确定 有针对性的干预措施如何影响老龄化的速度，以及行动能力和独立性的丧失。这一核心 从而为研究教育核心（REC）学者和试点研究调查人员提供支持。 衰老和疾病的特征是各种生理和代谢过程的逐渐恶化。 这与蛋白质、RNA和DNA的功能或含量的改变有关，这些蛋白质、RNA和DNA提供生物标志物， 监测老化。多种途径和结构域与衰老有关，如基因组不稳定性 （包括端粒磨损、突变和缺失）;表观遗传改变;蛋白质稳态丧失（包括 功能失调的自噬）;失调的营养感应;线粒体（Mt）功能障碍;炎症和 细胞衰老;干细胞衰竭，生物钟节律紊乱;和功能失调的烟酰胺 腺嘌呤二核苷酸（NAD+）稳态。蛋白质、RNA和DNA生物标志物的具体分析， 这个核心将提供与已知调节衰老和聚焦的主要生物和代谢途径相关的信息。 （i）Mt功能;（ii）炎症和衰老;（iii）自噬;（iv）生物钟;和 (v)NAD+稳态。我们使用创新的分析工具和标准的高通量分析， 确定衰老的基本生物学机制。代谢与转化科学核心 (RC2)支持总体假设，即特定蛋白质，RNA和DNA生物标志物的知识， 以及测量分离的线粒体和白色血细胞（WBC）的代谢，对于 了解健康老龄化的轨迹和流动性丧失的潜在生物学原因。 我们将支持蛋白质、RNA和DNA的提取;生物标志物的分析;细胞（WBC）的分离 和细胞器（线粒体）;和MT功能的评估。RC 2将为研究人员提供 OAIC核心和REC学者，具有成熟的方法;科学数据;基础设施;高素质 人员;咨询和协作专门知识。我们有丰富的历史，完成研究REC UF的学者和高级研究人员，以及全国各地的科学家。 RC 2追求以下目标： 目的1：支持蛋白质、RNA和DNA的分离和衰老特异性生物标志物的分析。 目的2：支持对线粒体呼吸、线粒体酶活性和NAD辅酶的分析。 目标3：促进和提供有关分析和样品储存的咨询，并协同合作 与内审办的其他核心一起，追求内审办促进流动性和独立性的共同主题。