Metabolism and Translational Science Core

代谢与转化科学核心

• 批准号: 10631863
• 负责人: CHRISTIAAN LEEUWENBURGH
• 金额: $ 24.52万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10631863
• 关键词: Aging; American; Ancillary Study; Autophagocytosis; Biochemical; Biological; Biological Markers; Biology; Blood; Blood capillaries; Brain; Cell Aging; Cell Separation; Cell physiology; Cells; Circadian Dysregulation; Clinical Research; Clinical Sciences; Coenzymes; Collaborations; Complementary DNA; Complex; Consultations; Country; DNA; DNA copy number; Data; Deterioration; Disease; Elderly; Electron Transport; Enzyme-Linked Immunosorbent Assay; Epigenetic Process; Fatty acid glycerol esters; Florida; Freezing; Future; Gene Expression Profiling; Genomic Instability; Genomics; Geroscience; Goals; Homeostasis; Human; Human Resources; Immunoassay; Impairment; Inflammation; Infrastructure; Intervention; Knowledge; Laboratories; Leukocytes; Longevity; Measures; Metabolic Pathway; Metabolism; Methodology; Mitochondria; Mitochondrial DNA; Molecular; Monitor; Muscle; Mutation; NADH; NADP; Nicotinamide adenine dinucleotide; Nuclear; Organelles; Pathway interactions; Pattern; Physical Function; Physiological Processes; Pilot Projects; Plasma; Principal Investigator; Protein Analysis; Proteins; Proteomics; Qualifying; RNA; Reaction; Reader; Recording of previous events; Research; Research Personnel; Research Support; Resolution; Resources; Respiration; Respiratory physiology; Sampling; Scientist; Sensory; Serum; Services; Spirometry; Techniques; Technology; Time; Tissue Sample; Tissues; Touch sensation; Translational Research; Universities; Vertebral column; Western Blotting; Whole Blood; age related; analytical tool; austin; biobank; biomarker selection; circadian pacemaker; cognitive function; design; detection of nutrient; detection platform; education research; enzyme activity; exhaustion; healthy aging; high standard; high throughput analysis; innovation; interest; metabolomics; mitochondrial dysfunction; nanoimmunoassay; novel; pre-clinical; proteostasis; research study; senescence; specific biomarkers; stem cells; telomere; transcriptomics

Summary The University of Florida (UF) Older Americans Independence Center (OAIC) supports the overall theme of “promotion of mobility and independence.” The Metabolism and Translational Science Core (RC2), in collaboration with our other UF OAIC cores, supports biochemical analyses for preclinical, human interventional, or observational clinical studies. By measuring a selected set of biomarkers, we can determine how targeted interventions influence the rate of aging, as well as loss of mobility and independence. This core thereby provides the support for the Research Education Core (REC) Scholars and pilot study investigators. Aging and disease feature progressive deterioration of various physiological and metabolic processes. This is associated with altered functions or contents of protein, RNA, and DNA, which provide biomarkers to monitor aging. Multiple pathways and domains have been associated with aging, such as genomic instability (including telomere attrition, mutations, and deletions); epigenetic alterations; loss of proteostasis (including dysfunctional autophagy); deregulated nutrient sensing; mitochondrial (Mt) dysfunction; inflammation and cellular senescence; stem cell exhaustion, disrupted circadian clock rhythms; and dysfunctional nicotinamide adenine dinucleotide (NAD+) homeostasis. The specific analyses of protein, RNA, and DNA biomarkers that this core will provide are related to major biological and metabolic pathways known to regulate aging and focus on: (i) Mt function; (ii) inflammation and senescence; (iii) autophagy; (iv) circadian clock biology; and (v) NAD+ homeostasis. We use innovative analytical tools and standard high-throughput analysis to determine the fundamental biological mechanisms of aging. The Metabolism and Translational Science Core (RC2) supports the overarching hypothesis that knowledge of specific protein, RNA, and DNA biomarkers, as well as measurements of metabolism of isolated mitochondria and white blood cells (WBCs), are critical for understanding the trajectory of healthy aging and the underlying biological causes of mobility loss. We will support extraction of proteins, RNA, and DNA; analysis of biomarkers; isolation of cells (WBCs) and organelles (mitochondria); and assessments of Mt function. RC2 will provide investigators across the OAIC Cores and REC Scholars with established methodologies; scientific data; infrastructure; highly qualified personnel; and consultative and collaborative expertise. We have a rich history of completing studies for REC Scholars and senior investigators at UF, as well as scientists around the country. RC2 pursues the following aims: Aim 1: To support protein, RNA, and DNA isolation and analysis of specific biomarkers of aging. Aim 2: To support analysis of Mt respiration, Mt enzyme activities, and NAD coenzymes. Aim 3: To facilitate and provide consultation on analyses and sample storage, and collaborate synergistically with the other OAIC cores to pursue the common OAIC theme of promotion of mobility and independence.

概括 佛罗里达大学 (UF) 美国老年人独立中心 (OAIC) 支持整体 主题是“促进流动性和独立性”。代谢和转化科学核心（RC2），在 与我们的其他 UF OAIC 核心合作，支持临床前、人类的生化分析 介入性或观察性临床研究。通过测量一组选定的生物标志物，我们可以确定 有针对性的干预措施如何影响衰老速度以及行动能力和独立性的丧失。这个核心 从而为研究教育核心（REC）学者和试点研究调查人员提供支持。 衰老和疾病的特点是各种生理和代谢过程逐渐恶化。 这与蛋白质、RNA 和 DNA 的功能或含量改变有关，这些蛋白质、RNA 和 DNA 为 监测老化。多种途径和领域与衰老相关，例如基因组不稳定 （包括端粒磨损、突变和缺失）；表观遗传改变；蛋白质稳态丧失（包括 自噬功能失调）；解除对营养物传感的管制；线粒体（Mt）功能障碍；炎症和 细胞衰老；干细胞耗竭、生物钟节律紊乱；和功能失调的烟酰胺 腺嘌呤二核苷酸 (NAD+) 稳态。对蛋白质、RNA 和 DNA 生物标志物的具体分析 该核心将提供与已知调节衰老和注意力的主要生物和代谢途径相关的信息 关于： (i) Mt 功能； (ii) 炎症和衰老； (三) 自噬； (iv) 生物钟生物学；和 (v) NAD+体内平衡。我们使用创新的分析工具和标准的高通量分析来 确定衰老的基本生物学机制。新陈代谢和转化科学核心 (RC2) 支持总体假设，即特定蛋白质、RNA 和 DNA 生物标志物的知识，如 以及分离线粒体和白细胞 (WBC) 代谢的测量对于 了解健康老龄化的轨迹以及行动能力丧失的根本生物学原因。 我们将支持蛋白质、RNA和DNA的提取；生物标志物分析；细胞（白细胞）的分离 和细胞器（线粒体）；和 Mt 功能评估。 RC2 将为调查人员提供 拥有既定方法的 OAIC 核心和 REC 学者；科学数据；基础设施;高素质 人员;以及咨询和协作专业知识。我们拥有完成 REC 学习的丰富历史 佛罗里达大学的学者和高级研究人员，以及全国各地的科学家。 RC2 追求以下目标： 目标 1：支持蛋白质、RNA 和 DNA 的分离以及特定衰老生物标志物的分析。 目标 2：支持 Mt 呼吸、Mt 酶活性和 NAD 辅酶的分析。 目标 3：促进分析和样品存储并提供咨询，并协同协作 与其他 OAIC 核心一起追求 OAIC 的共同主题：促进流动性和独立性。