RESOURCE CORE 2: METABOLISM AND BIOMARKERS CORE

资源核心 2：代谢和生物标志物核心

• 批准号: 8206034
• 负责人: CHRISTIAAN LEEUWENBURGH
• 金额: $ 13.83万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8206034
• 关键词: Address; Aging; Aging-Related Process; American; Animals; Apoptosis; Area; Autophagocytosis; Basic Science; Biochemical; Biochemistry; Biological; Biological Assay; Biological Factors; Biological Markers; Biological Process; Biometry; Clinical; Clinical Research; Clinical Sciences; Clinical Trials; Collaborations; Commit; Complex; Confocal Microscopy; Consultations; Data; Data Quality; Data Set; Deposition; Deterioration; Development; Disease; Education; Educational workshop; Elderly; Electron Transport; Ensure; Environment; Equipment; Evaluation; Fostering; Funding; Future; Goals; Grant; Human; Human Resources; In Situ; In Vitro; Inflammation; Instruction; Intervention; Iron; Knowledge; Knowledge Discovery; Laboratories; Laboratory Procedures; Laser Scanning Microscopy; Life; Measurement; Measures; Mentors; Metabolism; Methodology; Methods; Mitochondria; Modification; Molecular; Molecular Biology; Muscle; Muscle Fibers; Muscle function; Optics; Oxidative Stress; Pathogenesis; Pathway interactions; Penetration; Performance; Photobleaching; Physical Function; Physiological Processes; Play; Population; Prevention; Process; Qualifying; Research; Research Infrastructure; Research Personnel; Research Proposals; Resolution; Resources; Respiration; Risk Factors; Role; Sampling; Scientist; Sectioning technique; Signal Transduction Pathway; Skeletal Muscle; Techniques; Testing; Therapeutic; Tissue Sample; Training; Training and Infrastructure; Translational Research; age related; animal tissue; apoptosis deregulation; career development; data management; disability; experience; human subject; improved; innovation; interest; mitochondrial dysfunction; molecular marker; muscle form; new technology; novel; prevent; sarcopenia; skills; statistics; therapy design; training project; translational study

PROJECT SUMMARY The Older American Independence Center (OAIC) Metabolism and Biomarkers Core in collaboration with all other Cores, utilizes translational research to determine specific mechanisms of sarcopenia and the cause of reduced physical function in elderly populations. Sarcopenia is characterized by a progressive deterioration in various physiological and metabolic processes and is associated with lower physical function. Analyses performed by the Core focus on mitochondrial function, inflammation, oxidative stress, apoptosis and autophagy, biological factors implicated to cause aging. The Core supports the hypothesis that mitochondrial dysfunction, inflammation, oxidative stress and deregulation of apoptosis and autophagy are major causes of sarcopenia and disability. Supported research proposals will contain refined questions and utilize selected methodologies addressing potential causes of sarcopenia and altered physical function. Importantly, the Core is a central facility for acquiring research data and new laboratory skills. Training and instruction is provided either one on one or through organized workshops. By acquiring new laboratory skills and techniques Junior investigators and Pepper Scholars can further develop their research interests independently. In addition, the diversity of research experience and skills among personnel within the core as well as scientists utilizing its facilities provides a rich environment for scientific discussion and collaborations. The Core also provides consultations to scientists who are either interested in new areas of research or unfamiliar with certain techniques. Thus, this Core provides the infrastructure and training necessary to develop our understanding of the mechanisms contributing to the aging process. Furthermore, we are committed to fostering novel technologies in our pursuit of deciphering the central role that mitochondrial dysfunction plays in the pathogenesis of diseases and aging. To this end we have recently developed innovative intravital-multiphoton excitation laser-scanning microscopy and high-resolution respirometry techniques to assess mitochondrial function in intact freshly isolated small (20-40 mg) muscle samples. Thus, we are now able to determine mitochondrial function in-situ, which is more reflective of the natural state. Other areas of focus include inflammation, oxidative stress (including iron deposition), apoptosis and autophagy biomarkers. In summary, by measuring a small selected set of cellular and molecular markers in skeletal muscle tissue we can assess a unique and comprehensive spectrum of age-related alterations with the goal of determining the mechanisms contributing to sarcopenia.

项目摘要 老年美国独立中心（OAIC）代谢和生物标志物核心与所有其他核心合作，利用转化研究来确定肌肉减少症的具体机制和老年人群身体功能下降的原因。肌肉减少症的特征在于各种生理和代谢过程的进行性恶化，并且与较低的身体功能相关。由核心进行的分析集中在线粒体功能，炎症，氧化应激，细胞凋亡和自噬，涉及导致衰老的生物因素。核心支持线粒体功能障碍、炎症、氧化应激和细胞凋亡和自噬失调是肌肉减少症和残疾的主要原因的假设。支持的研究提案将包含精炼的问题，并利用选定的方法来解决肌肉减少症和身体功能改变的潜在原因。重要的是，核心是获取研究数据和新的实验室技能的中心设施。培训和指导是一对一或通过有组织的讲习班提供的。通过获得新的实验室技能和技术，初级研究人员和胡椒学者可以进一步独立发展他们的研究兴趣。此外，核心人员以及利用其设施的科学家的研究经验和技能的多样性为科学讨论和合作提供了丰富的环境。该中心还为对新的研究领域感兴趣或不熟悉某些技术的科学家提供咨询。因此，这个核心提供了必要的基础设施和培训，以发展我们对衰老过程机制的理解。此外，我们致力于培养新技术，以破译线粒体功能障碍在疾病和衰老发病机制中的核心作用。为此，我们最近开发了创新的体内多光子激发激光扫描显微镜和高分辨率呼吸测量技术，以评估完整的新鲜分离的小（20-40毫克）肌肉样本中的线粒体功能。因此，我们现在能够原位确定线粒体功能，这更能反映自然状态。其他关注领域包括炎症，氧化应激（包括铁沉积），细胞凋亡和自噬生物标志物。总之，通过测量骨骼肌组织中一小部分选定的细胞和分子标志物，我们可以评估年龄相关变化的独特和全面的谱，目的是确定导致肌肉减少症的机制。