RESOURCE CORE 2: METABOLISM AND BIOMARKERS CORE

资源核心 2：代谢和生物标志物核心

基本信息

批准号：
8206034
负责人：
CHRISTIAAN LEEUWENBURGH
金额：
$ 13.83万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-06-01 至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8206034
关键词：
Address Aging Aging-Related Process American Animals Apoptosis Area Autophagocytosis Basic Science Biochemical Biochemistry Biological Biological Assay Biological Factors Biological Markers Biological Process Biometry Clinical Clinical Research Clinical Sciences Clinical Trials Collaborations Commit Complex Confocal Microscopy Consultations Data Data Quality Data Set Deposition Deterioration Development Disease Education Educational workshop Elderly Electron Transport Ensure Environment Equipment Evaluation Fostering Funding Future Goals Grant Human Human Resources In Situ In Vitro Inflammation Instruction Intervention Iron Knowledge Knowledge Discovery Laboratories Laboratory Procedures Laser Scanning Microscopy Life Measurement Measures Mentors Metabolism Methodology Methods Mitochondria Modification Molecular Molecular Biology Muscle Muscle Fibers Muscle function Optics Oxidative Stress Pathogenesis Pathway interactions Penetration Performance Photobleaching Physical Function Physiological Processes Play Population Prevention Process Qualifying Research Research Infrastructure Research Personnel Research Proposals Resolution Resources Respiration Risk Factors Role Sampling Scientist Sectioning technique Signal Transduction Pathway Skeletal Muscle Techniques Testing Therapeutic Tissue Sample Training Training and Infrastructure Translational Research age related animal tissue apoptosis deregulation career development data management disability experience human subject improved innovation interest mitochondrial dysfunction molecular marker muscle form new technology novel prevent sarcopenia skills statistics therapy design training project translational study

项目摘要

PROJECT SUMMARY The Older American Independence Center (OAIC) Metabolism and Biomarkers Core in collaboration with all other Cores, utilizes translational research to determine specific mechanisms of sarcopenia and the cause of reduced physical function in elderly populations. Sarcopenia is characterized by a progressive deterioration in various physiological and metabolic processes and is associated with lower physical function. Analyses performed by the Core focus on mitochondrial function, inflammation, oxidative stress, apoptosis and autophagy, biological factors implicated to cause aging. The Core supports the hypothesis that mitochondrial dysfunction, inflammation, oxidative stress and deregulation of apoptosis and autophagy are major causes of sarcopenia and disability. Supported research proposals will contain refined questions and utilize selected methodologies addressing potential causes of sarcopenia and altered physical function. Importantly, the Core is a central facility for acquiring research data and new laboratory skills. Training and instruction is provided either one on one or through organized workshops. By acquiring new laboratory skills and techniques Junior investigators and Pepper Scholars can further develop their research interests independently. In addition, the diversity of research experience and skills among personnel within the core as well as scientists utilizing its facilities provides a rich environment for scientific discussion and collaborations. The Core also provides consultations to scientists who are either interested in new areas of research or unfamiliar with certain techniques. Thus, this Core provides the infrastructure and training necessary to develop our understanding of the mechanisms contributing to the aging process. Furthermore, we are committed to fostering novel technologies in our pursuit of deciphering the central role that mitochondrial dysfunction plays in the pathogenesis of diseases and aging. To this end we have recently developed innovative intravital-multiphoton excitation laser-scanning microscopy and high-resolution respirometry techniques to assess mitochondrial function in intact freshly isolated small (20-40 mg) muscle samples. Thus, we are now able to determine mitochondrial function in-situ, which is more reflective of the natural state. Other areas of focus include inflammation, oxidative stress (including iron deposition), apoptosis and autophagy biomarkers. In summary, by measuring a small selected set of cellular and molecular markers in skeletal muscle tissue we can assess a unique and comprehensive spectrum of age-related alterations with the goal of determining the mechanisms contributing to sarcopenia.

项目摘要美国较旧的美国独立中心（OAIC）代谢和生物标志物核心与所有其他核心合作，利用翻译研究来确定肌肉减少症的特定机制以及老年人群中身体机能降低的原因。肌肉减少症的特征是在各种生理和代谢过程中进行逐渐恶化，并且与身体功能较低有关。核心对线粒体功能，炎症，氧化应激，凋亡和自噬进行的分析，与导致衰老有关的生物学因素。核心支持以下假设：线粒体功能障碍，炎症，氧化应激以及对凋亡和自噬的失调是肌肉减少症和残疾的主要原因。支持的研究建议将包含精致的问题，并利用所选方法解决肌肉减少症的潜在原因并改变了身体机能。重要的是，核心是获取研究数据和新实验室技能的中心设施。一对一或通过有组织的研讨会提供培训和教学。通过获得新的实验室技能和技术，初级研究人员和胡椒学者可以独立发展他们的研究兴趣。此外，核心人员以及科学家利用其设施的研究经验和技能的多样性为科学讨论和协作提供了丰富的环境。核心还向对新研究领域感兴趣或不熟悉某些技术的科学家提供咨询。因此，该核心提供了必要的基础设施和培训，以发展我们对导致衰老过程的机制的理解。此外，我们致力于培养新技术，以破译线粒体功能障碍在疾病和衰老的发病机理中发挥作用的中心作用。为此，我们最近开发了创新的内部 - 多光子激发激光扫描显微镜和高分辨率呼吸测定技术，以评估完整的新鲜分离的小（20-40 mg）肌肉样品中的线粒体功能。因此，我们现在能够在原位确定线粒体功能，这更反映了自然状态。其他重点领域包括炎症，氧化应激（包括铁沉积），凋亡和自噬生物标志物。总而言之，通过测量骨骼肌组织中一组少量选定的细胞和分子标记，我们可以评估与年龄相关的变化的独特且全面的谱，以确定有助于肌肉减少症的机制。