RP2: Connectivity and Circuit Organization

RP2：连接和电路组织

• 批准号: 10226041
• 负责人: Martyn D Goulding
• 金额: $ 68.25万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-15 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10226041
• 关键词: 3-Dimensional; Anatomy; Area; Atlases; Behavior; Brain; Cells; Cervical; Cervical spinal cord structure; Coupled; Data; Data Science Core; Data Set; Development; Elbow; Electrophysiology (science); Forelimb; Genetic; Institutes; Interneurons; Lead; Life; Maps; Measurement; Modeling; Molecular; Motor; Motor Neurons; Motor output; Movement; Mus; Muscle; Neurons; Organizational Objectives; Output; Pathway interactions; Pattern; Periodicity; Population; Positioning Attribute; Reporter; Research; Resolution; Sensory; Shapes; Spinal; Study models; Synapses; System; Tracer; Weight; Whole-Cell Recordings; Work; Wrist; cell type; connectome; density; experimental study; information model; insight; motor behavior; motor control; mouse genetics; network models; neural circuit; neuronal circuitry; optogenetics; rabies viral tracing; relating to nervous system; sensory feedback; tool

Project Summary: Project 2 – Connectivity and Circuit Organization Neural circuits in the cervical spinal cord generate a range of rhythmic and dexterous motor behaviors that are essential for life. These circuits, aside from being important in their own right, serve as a window into how other brain areas are functionally organized by providing a critical readout of behavior, namely motor action. The analyses in this project will use cutting-edge genetic tools and anatomical approaches, many of which were developed at the Salk Institute, to create a scalable and interactive connectivity map of the pre- motor interneuron circuits in the cervical spinal cord. This connectivity map will be registered to motor pools innervating specific forelimb muscles in order for the connectivity datasets to be informative for the modeling and interpretation of motor behaviors that are key components of this Team SCC initiative. Intersectional mouse genetics will be used to target specific populations of spinal interneurons and annotate key information about position, and connectivity, in order to generate a physical wiring diagram for forelimb pre- motor circuits. The pre-motor wiring diagram will serve as a template for a subsequent more expansive analysis of spinal connectivity that will include sensory, propriospinal, and descending connections to forelimb pre-motor neurons. These studies, when completed, will provide unparalleled insights into the organization of the spinal motor circuitry and the motor system as a whole. They will also lead to a better understanding of the cellular interactions and neural computations that give rise to rhythmic and skilled forelimb movements.

项目摘要：项目 2 – 连接和电路组织 颈脊髓中的神经回路产生一系列有节律和灵巧的运动行为， 对生活至关重要。这些电路除了本身很重要之外，还可以作为了解 其他大脑区域如何通过提供行为的关键读数（即运动）来进行功能组织 行动。该项目的分析将使用尖端的遗传工具和解剖方法，其中许多 这是索尔克研究所开发的，旨在创建一个可扩展的交互式连接地图 颈脊髓的运动中间神经元回路。该连接图将被注册到汽车池 神经支配特定的前肢肌肉，以便连接数据集为建模提供信息 以及对运动行为的解释，这是该团队 SCC 计划的关键组成部分。交叉点 小鼠遗传学将用于针对特定的脊髓中间神经元群体并注释关键 有关位置和连接性的信息，以便生成前肢预连接的物理接线图 电机电路。电机前接线图将作为后续更广泛的接线图的模板 脊柱连接性分析，包括感觉、本体脊髓和下行连接 前肢前运动神经元。这些研究一旦完成，将为我们提供无与伦比的见解。 脊髓运动回路和运动系统作为一个整体的组织。他们也将带来更好的 了解细胞相互作用和神经计算，从而产生有节奏的和熟练的 前肢动作。