RP2: Connectivity and Circuit Organization

RP2：连接和电路组织

• 批准号: 10011919
• 负责人: Martyn D Goulding
• 金额: $ 68.25万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-15 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10011919
• 关键词: 3-Dimensional; Anatomy; Area; Atlases; Behavior; Brain; Cells; Cervical; Cervical spinal cord structure; Coupled; Data; Data Science Core; Data Set; Development; Elbow; Electrophysiology (science); Forelimb; Genetic; Institutes; Interneurons; Lead; Life; Maps; Measurement; Modeling; Molecular; Motor; Motor Neurons; Motor output; Movement; Mus; Muscle; Neurons; Organizational Objectives; Output; Pathway interactions; Pattern; Periodicity; Population; Positioning Attribute; Rabies; Rabies virus; Reporter; Research; Resolution; Sensory; Shapes; Spinal; Study models; Synapses; System; Tracer; Weight; Whole-Cell Recordings; Work; Wrist; cell type; connectome; density; experimental study; information model; insight; motor behavior; motor control; mouse genetics; network models; neural circuit; neuronal circuitry; optogenetics; relating to nervous system; sensory feedback; tool

Project Summary: Project 2 – Connectivity and Circuit Organization Neural circuits in the cervical spinal cord generate a range of rhythmic and dexterous motor behaviors that are essential for life. These circuits, aside from being important in their own right, serve as a window into how other brain areas are functionally organized by providing a critical readout of behavior, namely motor action. The analyses in this project will use cutting-edge genetic tools and anatomical approaches, many of which were developed at the Salk Institute, to create a scalable and interactive connectivity map of the pre- motor interneuron circuits in the cervical spinal cord. This connectivity map will be registered to motor pools innervating specific forelimb muscles in order for the connectivity datasets to be informative for the modeling and interpretation of motor behaviors that are key components of this Team SCC initiative. Intersectional mouse genetics will be used to target specific populations of spinal interneurons and annotate key information about position, and connectivity, in order to generate a physical wiring diagram for forelimb pre- motor circuits. The pre-motor wiring diagram will serve as a template for a subsequent more expansive analysis of spinal connectivity that will include sensory, propriospinal, and descending connections to forelimb pre-motor neurons. These studies, when completed, will provide unparalleled insights into the organization of the spinal motor circuitry and the motor system as a whole. They will also lead to a better understanding of the cellular interactions and neural computations that give rise to rhythmic and skilled forelimb movements.

项目概要：项目2 -连接和电路组织 颈脊髓中的神经回路产生一系列有节奏和灵巧的运动行为， 对生命至关重要。这些电路，除了本身很重要之外，还可以作为一个窗口， 其他大脑区域是如何通过提供行为的关键读数，即运动， 行动上该项目的分析将使用尖端的遗传工具和解剖方法，其中许多 这是在索尔克研究所开发的，以创建一个可扩展的和互动的连接地图的前， 颈脊髓中的运动中间神经元回路。这张连通图将被注册到车辆调配场 为特定前肢肌肉提供神经支配，以使连接数据集为建模提供信息 和运动行为的解释，这是这个团队SCC倡议的关键组成部分。交叉 小鼠遗传学将用于靶向脊髓中间神经元的特定群体， 关于位置和连接性的信息，以便生成前肢前的物理布线图， 电机电路电机前的接线图将作为模板，为随后的更广泛的 脊柱连接分析，包括感觉、脊髓本体和下行连接， 前肢前运动神经元这些研究一旦完成，将提供无与伦比的见解， 脊髓运动电路和运动系统的组织作为一个整体。它们也将带来更好的 理解细胞的相互作用和神经计算，产生节奏和熟练的 前肢运动