RP2: Connectivity and Circuit Organization

RP2：连接和电路组织

• 批准号: 10011919
• 负责人: Martyn D Goulding
• 金额: $ 68.25万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-15 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10011919
• 关键词: 3-Dimensional; Anatomy; Area; Atlases; Behavior; Brain; Cells; Cervical; Cervical spinal cord structure; Coupled; Data; Data Science Core; Data Set; Development; Elbow; Electrophysiology (science); Forelimb; Genetic; Institutes; Interneurons; Lead; Life; Maps; Measurement; Modeling; Molecular; Motor; Motor Neurons; Motor output; Movement; Mus; Muscle; Neurons; Organizational Objectives; Output; Pathway interactions; Pattern; Periodicity; Population; Positioning Attribute; Rabies; Rabies virus; Reporter; Research; Resolution; Sensory; Shapes; Spinal; Study models; Synapses; System; Tracer; Weight; Whole-Cell Recordings; Work; Wrist; cell type; connectome; density; experimental study; information model; insight; motor behavior; motor control; mouse genetics; network models; neural circuit; neuronal circuitry; optogenetics; relating to nervous system; sensory feedback; tool

Project Summary: Project 2 – Connectivity and Circuit Organization Neural circuits in the cervical spinal cord generate a range of rhythmic and dexterous motor behaviors that are essential for life. These circuits, aside from being important in their own right, serve as a window into how other brain areas are functionally organized by providing a critical readout of behavior, namely motor action. The analyses in this project will use cutting-edge genetic tools and anatomical approaches, many of which were developed at the Salk Institute, to create a scalable and interactive connectivity map of the pre- motor interneuron circuits in the cervical spinal cord. This connectivity map will be registered to motor pools innervating specific forelimb muscles in order for the connectivity datasets to be informative for the modeling and interpretation of motor behaviors that are key components of this Team SCC initiative. Intersectional mouse genetics will be used to target specific populations of spinal interneurons and annotate key information about position, and connectivity, in order to generate a physical wiring diagram for forelimb pre- motor circuits. The pre-motor wiring diagram will serve as a template for a subsequent more expansive analysis of spinal connectivity that will include sensory, propriospinal, and descending connections to forelimb pre-motor neurons. These studies, when completed, will provide unparalleled insights into the organization of the spinal motor circuitry and the motor system as a whole. They will also lead to a better understanding of the cellular interactions and neural computations that give rise to rhythmic and skilled forelimb movements.

项目摘要：项目2 - 连接性和电路组织 颈椎中的神经回路产生一系列节奏和灵巧的运动行为 对生活至关重要。这些电路除了自己的重要性之外，还可以作为进入的窗口 其他大脑区域如何通过提供行为的重要读数（即电动机）来组织功能 行动。该项目中的分析将使用尖端的遗传工具和解剖方法，许多 这是在Salk Institute开发的，以创建前pre- 颈脊髓中的运动中神经元电路。该连接图将注册到电动池 支配特定的前肢肌肉，以使连通性数据集为建模提供信息 并解释是该团队SCC计划的关键组成部分的运动行为。交叉 小鼠遗传学将用于靶向特定的脊髓中间神经元和注释键 有关位置和连接性的信息，以生成前肢的物理接线图 电机电路。电动机接线图将用作随后更膨胀的模板 分析脊柱连接性，包括感官，普遍性以及与 前动机神经元。这些研究完成后将提供无与伦比的见解 脊柱电路电路和整个电机系统的组织。他们也会带来更好的 了解引起节奏和熟练的细胞相互作用和神经收费 前肢运动。