通过直接捕获、标记和检测单个病毒颗粒来快速诊断和定量 HIV-猫眼课题宝

通过直接捕获、标记和检测单个病毒颗粒来快速诊断和定量 HIV

批准号：

10227771

负责人：

Ashutosh Chilkoti

金额：

$44.43万

依托单位：

DUKE UNIVERSITY

依托单位国家：

美国

项目类别：

财政年份：

2019

资助国家：

美国

项目状态：

已结题

起止时间：

2019-08-01 至 2023-07-31

来源：

https://reporter.nih.gov/project-details/10227771

关键词：

Acquired Immunodeficiency Syndrome Acute Antibodies Awareness Binding Binding Sites Biological Assay Blood Cellular Phone Cessation of life Chronic Clinical Clinical Trials Country Detection Development Devices Diagnosis Diagnostic Diagnostic tests Diffusion Disease Disease Management Early Diagnosis Engineering Ensure Equipment Event Exposure to Future Goals Gold HIV HIV Infections HIV diagnosis Human Human immunodeficiency virus test Image Individual Infection Label Lectin Maintenance Membrane Proteins Microfluidics Microscopy Molecular Conformation Monitor Names Outcome Patients Performance Persons Phase Plasma Polysaccharides Prevention RNA Rapid diagnostics Reagent Sampling Sensitivity and Specificity Specificity Spottings Surface Technology Testing Time Training Validation Variant Viral Viral Antigens Viral Load result Virion Virus Virus Diseases acute infection antiretroviral therapy assay development base biobank cost diagnostic assay fluorescence imaging improved low and middle-income countries next generation novel pandemic disease point-of-care diagnostics prevent programs prototype rapid diagnosis self diagnosis self testing testing uptake viral RNA viral rebound

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中文摘要

翻译

摘要我们建议开发一种先进的艾滋病毒快速护理点诊断。这种方法将利用一种新的D4诊断分析平台：D4之所以如此命名，是因为D4的链添加血液时发生的事件：（1）将血液分配到芯片上;（2）“可溶性” （3）包被的病毒粒子在表面上的扩散和与特异性抗体的结合捕获抗体斑点;（4）用荧光成像检测结合。我们将增强艾滋病毒粒子使用凝集素Griffithsin捕获，它可以结合HIV病毒粒子刺突的聚糖盾，诱导可被抗体捕获的CD 4结合位点的暴露。单个病毒体可以使用下一代手机显微镜进行捕获、标记和成像。这诊断测试不仅能够实现独立于患者的快速“窗口独立”诊断，抗体，但也可能用于常规病毒载量监测。

英文摘要

Abstract We propose to develop an advanced rapid point of care diagnostic for HIV. This approach will leverage a novel D4 diagnostic assay platform: The D4 is so named because of the chain of events that occur upon addition of blood: (1) Dispense blood onto chip; (2) Dissolution of “soluble” detection reagent spots (3) Diffusion of coated virion across a surface and binding to specific capture Ab spots; (4) Detection of binding with fluorescence imaging. We will enhance HIV virion capture using a lectin, Griffithsin, which can bind to the glycan shield of HIV virions spikes and induce exposure of CD4 binding sites which can be captured by antibodies. Individual virions can be captured, labelled and imaged using next generation cell phone based microscopy. This diagnostic test enables not only rapid “window independent” diagnosis independent of patient antibodies, but also potentially for routine viral load monitoring.

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