Multiplex point-of-care test for diagnosis, prognosis and serology of COVID19
用于 COVID19 诊断、预后和血清学的多重即时检测
基本信息
- 批准号:10417262
- 负责人:
- 金额:$ 48.75万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-06-03 至 2024-05-31
- 项目状态:已结题
- 来源:
- 关键词:2019-nCoVAddressAdsorptionAntibodiesAntigensArchivesAreaBiological AssayBiological MarkersBloodBlood specimenBuffersCCL7 geneCOVID-19COVID-19 detectionCOVID-19 diagnosisCOVID-19 patientCXCL10 geneCellsClinicalCollectionContact TracingCustomDataDetectionDeteriorationDevelopmentDevicesDiagnosisDropsEarly DiagnosisEnsureExposure toFibrin fragment DFluorescenceForce of GravityGeometryGlassGlycolsGoldHandHealthHomeHumanImageImmunoassayIndividualInjectionsIntelligenceInterleukin-10Interleukin-6InterventionLabelLengthLiquid substanceMethacrylatesMicrofluidicsMoldsMonitorNoiseNoseNucleocapsidNucleocapsid ProteinsPatient CarePatient MonitoringPatientsPerformancePharyngeal structurePlasmaPolymethyl MethacrylatePopulationPrevalencePrintingProceduresPrognosisProteinsQuarantineReagentRecombinantsReproducibilityResistanceRiskRunningSARS-CoV-2 antibodySARS-CoV-2 antigenSARS-CoV-2 infectionSamplingScanningSecureSensitivity and SpecificitySerologySerology testSerumSevere Acute Respiratory SyndromeSeveritiesSignal TransductionSpottingsSurfaceSwabSymptomsTNF geneTestingTextThickTimeValidationViralViral AntigensWhole Bloodantibody detectionantigen detectionassay developmentcloud platformcostdetection limitdetectorepidemiology studyfight againstfluorescence imaginghigh riskhigh throughput screeningimaging platformlateral flow assaymanufacturing processmultiplex detectionnasal swaboropharyngeal swabperformance testspoint of care testingpoint-of-care diagnosticsprognosticrapid diagnosisreceptor bindingsample archivescreeningtool
项目摘要
ABSTRACT
The objective of this proposal is to develop and validate a new set of low-cost, easy-to-use, point-of-care tests
(POCTs) that can simultaneously detect multiple biomarkers within 30 minutes with high sensitivity and specificity
to enable early diagnosis, prognosis, and serology of COVID19 patients. The multiplexed D4-POCT employs
antibodies for antigen detection in the diagnosis and prognosis chips, and antigens for antibody detection in the
serology chip. Capture and detection antibodies will be used to generate assays for S1 and N antigens from
nasal/throat swab collection media for diagnosis, D-dimer, IL-2R, IL-6, IL-10 and TNF-α in human blood or serum
for prognosis, and S1, N, and RBD viral antigens will be used as capture and detection reagents in a double-
antigen serological assay to detect anti-SARS-CoV-2 antibodies in human blood and serum. Optimal reagents
for assay development will be identified by leveraging the multiplexing capabilities of the D4-POCT to perform
high-throughput screening and pairing of patient derived antibodies and viral antigens. The D4-POCT has three
components. The first component—the D4 chip—is fabricated by inkjet printing microspots of capture and
fluorescently labeled detection reagents (antibodies for antigen detection or antigens for antibody detection)
directly on a protein- and cell-resistant poly(oligoethylene glycol methacrylate) brush that is grafted from the
surface of glass, which reduces background noise, yielding pg/mL limit-of-detection and over 4 logs of dynamic
range. The second component is a gravity-driven microfluidics cassette that encases the D4 chip and is
fabricated by low-cost and high-throughput injection molding. The microfluidics cassette reduces user exposure
to potentially infectious material and minimizes user interaction, as it only requires the addition of a drop of
sample into the sample inlet and two drops of wash buffer into the wash buffer inlet —thereby mimicking the
procedure used to carry out lateral flow assays, the gold standard of immunoassay for field use. The chip
automatically performs a timed incubation and rinse step that removes the sample and any unbound detection
reagent from the surface of the D4 chip, leaving the central area of the D4 chip ready for imaging by the third
component—the D4Scope—a customized, low-cost, hand-held fluorescence detector that we have developed.
The fluorescent spot intensities on the D4 chip are converted to analyte concentration by a built-in “App” on the
D4Scope, and analyte concentrations and the raw data —the images— are both automatically uploaded to a
secure cloud server. All components of the D4-POCT are easy to fabricate at high volumes as the chips are
rapidly manufactured by inkjet printing picograms of reagents per test, the microfluidics cassette is fabricated by
injection molding, and the D4Scope is assembled from off-the shelf parts. The impact of this proposal will be
the development and clinical validation of POCTs for diagnosis, prognosis and serology—all built on the same
platform— that can be globally deployed in the fight against COVID-19.
摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Ashutosh Chilkoti其他文献
Ashutosh Chilkoti的其他文献
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{{ truncateString('Ashutosh Chilkoti', 18)}}的其他基金
Development, Clinical Validation, and Readiness for Implementation of a Novel Mp1p D4 Poin Diagnosis of Talaromycosist of Care Test for Rapid
新型 Mp1p D4 点诊断踝部真菌护理测试的开发、临床验证和准备实施
- 批准号:
10700281 - 财政年份:2023
- 资助金额:
$ 48.75万 - 项目类别:
Multiplex point-of-care test for diagnosis, prognosis and serology of COVID19
用于 COVID19 诊断、预后和血清学的多重即时检测
- 批准号:
10297706 - 财政年份:2021
- 资助金额:
$ 48.75万 - 项目类别:
Multiplex point-of-care test for diagnosis, prognosis and serology of COVID19
用于 COVID19 诊断、预后和血清学的多重即时检测
- 批准号:
10641013 - 财政年份:2021
- 资助金额:
$ 48.75万 - 项目类别:
Injectable PEG-like Conjugate for Sustained Delivery of a Peptide Drug for Type 2 Diabetes Treatment
用于持续递送肽药物治疗 2 型糖尿病的可注射 PEG 样缀合物
- 批准号:
10314066 - 财政年份:2020
- 资助金额:
$ 48.75万 - 项目类别:
Injectable PEG-like Conjugate for Sustained Delivery of a Peptide Drug for Type 2 Diabetes Treatment
用于持续递送肽药物治疗 2 型糖尿病的可注射 PEG 样缀合物
- 批准号:
10520019 - 财政年份:2020
- 资助金额:
$ 48.75万 - 项目类别:
Point-of-care cellular and molecular pathology of breast tumors on a cell phone
在手机上进行乳腺肿瘤的护理点细胞和分子病理学
- 批准号:
10358633 - 财政年份:2020
- 资助金额:
$ 48.75万 - 项目类别:
A Fully Integrated Point-of-Care Test for Ebola
完全集成的埃博拉即时护理测试
- 批准号:
10269019 - 财政年份:2020
- 资助金额:
$ 48.75万 - 项目类别:
A Fully Integrated Point-of-Care Test for Ebola
完全集成的埃博拉即时护理测试
- 批准号:
10468131 - 财政年份:2020
- 资助金额:
$ 48.75万 - 项目类别:
A Fully Integrated Point-of-Care Test for Ebola
完全集成的埃博拉即时护理测试
- 批准号:
10119782 - 财政年份:2020
- 资助金额:
$ 48.75万 - 项目类别:
Point-of-care cellular and molecular pathology of breast tumors on a cell phone
在手机上进行乳腺肿瘤的护理点细胞和分子病理学
- 批准号:
10586029 - 财政年份:2020
- 资助金额:
$ 48.75万 - 项目类别:
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