Single-session Introduction of Mutations in Parallel Lines (SIMPL)

平行线突变的单次引入 (SIMPL)

• 批准号: DP210101957
• 负责人: Prof Lars Ittner
• 金额: $ 48.07万
• 依托单位: Macquarie University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2021
• 资助国家: 澳大利亚
• 起止时间: 2021-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP210101957
• 关键词: Single; session; Introduction; Mutations; Parallel

This project aims to develop a novel method for markedly accelerating production of genetically modified mice, which are a key 'tool' for studying biological processes and diseases. The work plans to take CRISPR, the latest gene-editing technique, to the next level by developing a novel CRISPR-based method to generate different mouse strains with distinct variations of the same gene sequences, at a fraction of the present cost and time. This project should overcome a major barrier to studying gene function with unprecedented detail, thereby opening new avenues for future research into biological processes. Thus, the outcomes from this project should impact on the entire field of biomedical research, and advance Australia's biotech industry.

该项目旨在开发一种新的方法，显著加快转基因小鼠的生产，转基因小鼠是研究生物过程和疾病的关键工具。这项工作计划将最新的基因编辑技术CRISPR提升到一个新的水平，开发一种基于CRISPR的新方法，以目前成本和时间的一小部分来产生具有相同基因序列的不同变异的不同小鼠品系。这个项目应该会克服以前所未有的细节研究基因功能的主要障碍，从而为未来对生物过程的研究开辟新的途径。因此，该项目的成果将影响整个生物医学研究领域，并推动澳大利亚的生物技术产业。