Bioelectrochemistry at the Nanoscale: Fundamentals and Applications

项目摘要 21世纪医学和生化领域的关键问题集中在亲密手术上 以及分子水平上的生物分子的功能。通过了解的物理属性 生物分子在纳米尺度上，我们可以设想如何控制它们并将它们用于各种应用。 下面的提案旨在通过使用纳米级电化学来促进这些关键问题的解决。我 我非常兴奋地与你们分享我们在处理这两个问题上的一些初步结果和未来愿景 生物电化学的应用和基础方面。提案的第一部分集中在合并上 该实验室在纳米电化学和电荷传输现象方面拥有专业知识，并专注于一种创新的 方法测量单个酶在催化过程中的电子响应。通过这样做，我们希望 用电子手段解开酶相对于其催化作用的运作和波动的方式 分子水平上的活性。获得的信息可以指导我们对酶的控制和设计 无论是从基础方面还是从工业应用方面。 研究计划的第二部分为我们思考方式的范式转变提供了一个模板 电化学生物传感器。我们建议构建一种化学放大的电化学系统 能够一次检测一种绝缘材料并分析其大小。我们计划利用这一点 病原体快速检测传感器的实验设计方法。这里的主要目标是 创新一种可以以个性化方式使用的传感器，而不需要复杂的设备操作。 这种传感器的实现可以潜在地减少在这一点上给予的不必要抗生素的数量 关爱。

Project Summary Key questions of the 21st century in medicinal and biochemical areas are focused on the intimate operation and functionality of biomolecule at the molecular level. By understanding the physical properties of biomolecules at the nanoscale we can envision how to control them and use them for various applications. The proposal below is aimed to promote these key questions via the use of nanoscale electrochemistry. I am very excited to share with you some of our preliminary results and future vision dealing with both the applied and fundamental sides of Bioelectrochemistry. The first part of the proposal is focused on merges the lab expertise in nanoelectrochemistry and charge transport phenomena and focuses on an innovative method to measure the electronic response of individual enzymes during catalysis. By that, we hope to unravel by electronic means the way that enzymes operate and fluctuate with respect to their catalytic activity at the molecular level. Gained information can guide us towards the control and design of enzymes both from fundamental aspects and industrial applications. The second part of the research program offers a template for a paradigm shift in the way we think about electrochemical biosensors. We propose to construct a chemically amplified electrochemical system that enables the detection and analytical sizing of insulating materials, one at a time. We plan to use this experimental approach for designing a sensor for rapid pathogen detection. The main goal here is to innovate a sensor that can be used in a personalized fashion, without the need of complex device operation. Realization of such sensor can potentially mitigate the number of unnecessary antibiotics given at the point of care.