Bioelectrochemistry at the Nanoscale: Fundamentals and Applications

纳米级生物电化学：基础知识和应用

• 批准号: 10703421
• 负责人: Lior Sepunaru
• 金额: $ 34.2万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-23 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10703421
• 关键词: Antibiotics; Area; Biochemical; Biological; Biosensor; Catalysis; Charge; Chemicals; Complex; Detection; Development; Devices; Electrochemistry; Electronics; Enzymes; Future; Generations; Goals; Individual; Industrialization; Kinetics; Measures; Medicine; Methods; Molecular; Oxidation-Reduction; Pathogen detection; Process; Reaction; Research; Side; System; Techniques; Thermodynamics; Time; Vision; communicable disease diagnosis; design; individual response; innovation; nanoscale; operation; physical property; point of care; programs; rapid diagnosis; sensor; tool

Project Summary Key questions of the 21st century in medicinal and biochemical areas are focused on the intimate operation and functionality of biomolecule at the molecular level. By understanding the physical properties of biomolecules at the nanoscale we can envision how to control them and use them for various applications. The proposal below is aimed to promote these key questions via the use of nanoscale electrochemistry. I am very excited to share with you some of our preliminary results and future vision dealing with both the applied and fundamental sides of Bioelectrochemistry. The first part of the proposal is focused on merges the lab expertise in nanoelectrochemistry and charge transport phenomena and focuses on an innovative method to measure the electronic response of individual enzymes during catalysis. By that, we hope to unravel by electronic means the way that enzymes operate and fluctuate with respect to their catalytic activity at the molecular level. Gained information can guide us towards the control and design of enzymes both from fundamental aspects and industrial applications. The second part of the research program offers a template for a paradigm shift in the way we think about electrochemical biosensors. We propose to construct a chemically amplified electrochemical system that enables the detection and analytical sizing of insulating materials, one at a time. We plan to use this experimental approach for designing a sensor for rapid pathogen detection. The main goal here is to innovate a sensor that can be used in a personalized fashion, without the need of complex device operation. Realization of such sensor can potentially mitigate the number of unnecessary antibiotics given at the point of care.

项目摘要 世纪医学和生物化学领域的关键问题集中在亲密操作 和生物分子的功能性。通过了解 在纳米级的生物分子中，我们可以想象如何控制它们并将它们用于各种应用。 下面的建议旨在通过使用纳米电化学来促进这些关键问题。我 我很高兴能与大家分享我们的一些初步成果和未来的愿景， 生物电化学的应用和基础方面。提案的第一部分侧重于合并 纳米电化学和电荷传输现象的实验室专业知识，并专注于创新 一种测量单个酶在催化过程中的电子响应的方法。因此，我们希望 通过电子手段解开酶的运作方式，并就其催化活性波动 分子水平上的活动。获得的信息可以指导我们控制和设计酶 从基础方面和工业应用方面。 研究计划的第二部分为我们思考的方式提供了一个范式转变的模板 电化学生物传感器我们建议构建一个化学放大的电化学系统， 能够一次检测一种绝缘材料并对其进行分析。我们计划用这个 设计用于快速病原体检测的传感器的实验方法。这里的主要目标是 创新一种传感器，可以以个性化的方式使用，而不需要复杂的设备操作。 这种传感器的实现可以潜在地减少在该点给予的不必要的抗生素的数量。 照顾。

项目成果

Direct Electricity Production from Nematostella and Arthemia's Eggs in a Bio-Electrochemical Cell.

• DOI: 10.3390/ijms232315001
• 发表时间: 2022-11-30
• 期刊: International journal of molecular sciences
• 影响因子: 5.6

Precise Electrochemical Sizing of Individual Electro-Inactive Particles.

单个电惰性颗粒的精确电化学尺寸测定。

• DOI: 10.3791/65116
• 发表时间: 2023
• 期刊: Journal of visualized experiments : JoVE
• 作者: Chung,Julia;Plaxco,KevinW;Sepunaru,Lior
• 通讯作者: Sepunaru,Lior

Calibration-Free, Seconds-Resolved In Vivo Molecular Measurements using Fourier-Transform Impedance Spectroscopy Interrogation of Electrochemical Aptamer Sensors.

• DOI: 10.1021/acssensors.3c00632
• 发表时间: 2023-08-25
• 期刊: ACS SENSORS
• 影响因子: 8.9
• 作者: Roehrich, Brian;Leung, Kaylyn K.;Gerson, Julian;Kippin, Tod E.;Plaxco, Kevin W.;Sepunaru, Lior
• 通讯作者: Sepunaru, Lior