NIH TCF Dev and Prod of SARS-CoV-2 Spike tetramers for B Cell Detection

用于 B 细胞检测的 SARS-CoV-2 Spike 四聚体的 NIH TCF 开发和生产

• 批准号: 10282181
• 负责人: JOHN ALTMAN
• 金额: $ 59.94万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-03-21 至 2027-03-20
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10282181
• 关键词: 2019-nCoV; Antigen Targeting; B-Lymphocytes; CD8-Positive T-Lymphocytes; Communities; Core Facility; Detection; Development; Evaluation; MHC Class I Genes; Patients; Population; Process; Production; Proteins; Reagent; Research; T-Lymphocyte; T-Lymphocyte Epitopes; Technology; United States National Institutes of Health; adaptive immune response; coronavirus disease; improved; research and development; response; tool; vaccine candidate

The NIH Tetramer Core Facility (TCF) distributes high quality reagents for detection of innate T cell and classical CD4 and CD8 T cell populations. This Facility also conducts R&D to produce new reagents and improve existing tetramer technologies. The TCF is producing MHC class I and class II tetramers for isolation and characterization of SARS-CoV2-specific CD4 and CD8 T cells, and is also developing SARS-CoV-2 Spike protein tetramers for detection and characterization of SARS-CoV2 specific B cells. Thus far, 56 MHC tetramers have been distributed to the community, an additional 35 are in-process. Orders are expected to increase as COVID-related research increases. Additional reagents will be produced and distributed as new T cell epitopes and B cell antigen targets are identified. These tetramer reagents will be invaluable tools for evaluation of adaptive immune responses in COVID patients or in response to candidate vaccines.

NIH Tetramer Core Facility（TCF）提供高质量的试剂，用于检测先天性T细胞和经典的CD 4和CD 8 T细胞群。 该设施还进行研发，以生产新试剂和改进现有的四聚体技术。 TCF正在生产MHC I类和II类四聚体，用于分离和鉴定SARS-CoV 2特异性CD 4和CD 8 T细胞，还在开发SARS-CoV-2刺突蛋白四聚体，用于检测和鉴定SARS-CoV 2特异性B细胞。到目前为止，已经向社区分发了56个MHC四聚体，另外35个正在进行中。随着新冠肺炎相关研究的增加，预计订单将增加。随着新的T细胞表位和B细胞抗原靶标的鉴定，将生产和分发额外的试剂。 这些四聚体试剂将成为评估COVID患者适应性免疫应答或候选疫苗应答的宝贵工具。