Informing Cancer Epidemiology Through the Identification of Differentiating Chemical Constituents of Cigarillo Smoke

通过鉴定小雪茄烟雾的化学成分来了解癌症流行病学

• 批准号: 10281630
• 负责人: Theodore P. Klupinski
• 金额: $ 40.99万
• 依托单位: BATTELLE CENTERS/PUB HLTH RES & EVALUATN
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-16 至 2023-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10281630
• 关键词: Adult; Advanced Malignant Neoplasm; Affect; Age; Analytical Chemistry; Cannabis; Carcinogens; Chemicals; Cigar; Cigar Smoking; Cigarette; Complement; Data; Databases; Elderly; Environmental Risk Factor; Epidemiology; Exhibits; Exposure to; Future; Gas Chromatography; Health; Individual; Investigation; Knowledge; Light; Mainstreaming; Malignant neoplasm of esophagus; Malignant neoplasm of liver; Malignant neoplasm of lung; Malignant neoplasm of pharynx; Malignant neoplasm of salivary gland; Minority; Outcome; Pattern; Population; Population Assessment of Tobacco and Health; Public Health; Reporting; Research; Research Project Grants; Resources; Risk; Sales; Sampling; Signal Transduction; Smoke; Smoker; Smoking; Techniques; Time; Tobacco smoke; Tobacco use; United States Environmental Protection Agency; Youth; advanced analytics; anticancer research; biomarker development; cancer epidemiology; cancer risk; cancer type; carcinogenicity; chemical standard; cigarette smoke; cigarette smoking; cigarillos; computational toxicology; dashboard; design; epidemiologic data; epidemiology study; improved; innovation; malignant tongue neoplasm; population based; screening; time of flight mass spectrometry; tobacco products; two-dimensional; young adult

Project Summary Cigar smoking is associated with multiple negative health outcomes, including increased risks for various types of cancer. Cigar use in the U.S. increased between 2000 and 2011 even as cigarette sales declined over the same time period. Cigar use is common among minority youth and as a vehicle to administer cannabis. Within the product class of cigars, cigarillos are the most commonly used in the U.S. and are associated with specific public health concerns, including higher rates of use by young adults (ages 18–24) in contrast to older adults and different patterns of co-use of multiple tobacco products. Thus, it is important to improve the understanding of particular factors related to cigarillo use in order to inform cancer epidemiology. In the proposed research, we will identify the chemical constituents of mainstream cigarillo smoke that are different than those of mainstream cigarette smoke through non-targeted chemical analysis (i.e., the identification of chemicals that are not specified prior to analyzing a given sample). The expected results are important for epidemiological purposes in that they can reveal carcinogens in cigarillo smoke beyond those known to be commonly associated with tobacco smoke in general, thus providing a better understanding of environmental risk factors in epidemiological research. Furthermore, the identified compounds can be used in later research projects to inform the development of biomarkers of exposure that can be used in individual or population base sampling to estimate cigarillo exposure. To achieve our research objective, we will employ two-dimensional gas chromatography–time-of-flight mass spectrometry (GC×GC-TOFMS), an advanced analytical chemistry technique with powerful capabilities for non-targeted chemical analysis. GC×GC-TOFMS will be used to collect non-targeted analytical chemistry data for both cigarillo smoke and cigarette smoke. The data from the two product (sub)classes will be compared against one another, and those signals that differentiate the product (sub)classes will be assessed using computational toxicology databases (e.g., the Environmental Protection Agency CompTox Chemicals Dashboard) to determine which are most likely from compounds that would present significant carcinogenicity concerns in cigarillo smoke. Additional analyses will then be performed using authentic chemical standards to confirm the chemical identities and to quantify the amounts in both cigarillo smoke and cigarette smoke. The proposed innovative research is designed to identify and quantify specific chemical constituents (1) that differentiate cigarillo smoke from cigarette smoke, and (2) that are potentially carcinogenic. The results will complement epidemiological data regarding the impact to public health associated with cigarillo use. This investigation will thus advance cancer research through expanding knowledge about cancer risks for those populations most affected by cigarillo smoking.

项目摘要 吸烟与多种负面健康结果有关，包括增加各种类型的风险， 癌2000年至2011年，美国的卷烟使用量增加，而同期卷烟销售量下降。 时间段大麻在少数民族青年中很常见，并被用作施用大麻的工具。内 雪茄的产品类别，雪茄是最常用的在美国和与特定的公众 健康问题，包括年轻人（18-24岁）使用率高于老年人， 多种烟草产品共同使用的不同模式。因此，重要的是要提高认识， 与雪茄使用相关的特殊因素，以告知癌症流行病学。 在拟议的研究中，我们将确定主流雪茄烟的化学成分， 通过非目标化学分析与主流香烟烟雾的那些不同（即，识别 在分析给定样品之前未指定的化学品）。预期的结果对以下方面很重要： 流行病学目的，因为它们可以揭示雪茄烟中的致癌物， 通常与烟草烟雾有关，从而更好地了解环境 流行病学研究中的危险因素。此外，所鉴定的化合物可用于以后的研究 为制定可用于个人或人群的接触生物标志物提供信息的项目 采样以估计雪茄暴露。 为了实现我们的研究目标，我们将采用二维气相色谱-飞行时间质谱联用技术， GC×GC-TOFMS是一种先进的分析化学技术，具有强大的非靶向分析能力， 化学分析GC×GC-TOFMS将用于收集两种雪茄的非靶向分析化学数据 烟雾和香烟烟雾。两个产品（子）类别的数据将相互比较， 将使用计算毒理学数据库评估区分产品（子）类别的信号 (e.g.,环境保护局CompTox化学品仪表板），以确定哪些最有可能来自 这些化合物在小雪茄烟雾中会产生显著的致癌性问题。随后将进行其他分析， 使用真实的化学标准品进行，以确认化学特性并量化两者中的量。 雪茄烟和香烟烟雾。 拟议的创新研究旨在确定和量化特定的化学成分（1）， 小雪茄烟从香烟烟雾，和（2）是潜在的致癌物质。结果将补充 关于使用小雪茄对公众健康影响的流行病学数据。因此，这项调查将 通过扩大受癌症影响最严重的人群的癌症风险知识，推进癌症研究。 抽雪茄。