Three-Photon Microscope for High-Resolution Deep Tissue Imaging
Three-Photon Microscope for High-Resolution Deep Tissue Imaging
批准号:
10282619
负责人:
Sava Sakadzic
金额:
$59.99万
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
已结题
起止时间:
2021-08-15 至 2022-08-14
关键词:
ArchitectureAreaBiologicalBiomedical ResearchBlood VesselsBostonBrainCalcium SignalingCommunitiesDevelopmentDiseaseFundingGeneral HospitalsHealthHippocampus (Brain)HumanImageImaging technologyLasersMassachusettsMicroscopeMissionModificationNational Institute of Mental HealthPhotonsPhysiologic pulseResearchResearch PersonnelResolutionRodentStructureTechnologyTissue imagingTissuesUnited States National Institutes of Healthbioimagingclinical imagingimaging capabilitiesimaging modalityimaging systemin vivoinstrumentinstrumentationnoveloptical imagingpre-clinicalrelating to nervous systemresearch studythree photon microscopytwo photon microscopywhite matter
中文摘要
项目总结
英文摘要
Project Summary
Three-photon microscopy (3PM) is an optical imaging technology that enables significantly deeper imaging in a
scattering biological tissue than the current leading standard two-photon microscopy (2PM), while providing
additional contrast mechanisms and enhancing spatial resolution. This technology is made possible by advances
in long-wavelength, tunable, high-pulse-energy femtosecond lasers and corresponding modifications in 2PM
setups to accommodate a novel excitation regime.
As a direct result of these advancements, 3PM imaging depth in rodent brain is greatly extended with respect to
2PM imaging depth—from ~700 μm to more than 1,200 μm. Due to such a large improvement, we anticipate
that 3PM will allow breakthrough studies of the structure (e.g., vascular, glial, and neural architecture) and
function (e.g., oxygenation, calcium signaling, and cellular interactions) in preclinical in vivo studies, with cellular-
size resolution in previously inaccessible tissue layers, such as the subcortical white matter and the
hippocampus. The 11 NIH-funded projects listed in this proposal will significantly benefit from these imaging
capabilities. These projects span a diverse range of research topics relevant to NIMH mission, including
improvement of the quantitativeness of the clinical imaging modalities and better understanding of the
mechanisms involved in development of various brain conditions.
The Martinos Center for Biomedical Imaging and Massachusetts General Hospital constitute a diverse
community of researchers, many of whom are funded with NIH support, performing cutting edge biomedical
research that will further advance our fundamental understanding of human health and disease. This proposed
3PM imaging system will represent the first instrument of its kind in the Boston area (and probably Northeast
US) that is available within an Imaging Core. This cutting-edge instrumentation will allow our research community
to significantly enhance various research studies relevant to NIMH mission, and it will also allow the Martinos
Center to continue prospering as a leader for advanced biomedical imaging.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Two-photon Imaging of Oxygen and Blood Flow in Retinal and Cerebral Vasculature
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批准号:10184187
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项目类别:
-
资助金额:$189.72万
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财政年份:2021
-
负责人:Sava Sakadzic
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依托单位:
Two-Photon Microscopy of Oxygen Consumption in the Brain
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批准号:8985334
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项目类别:
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资助金额:$38.44万
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财政年份:2015
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负责人:Sava Sakadzic
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依托单位:
Two-Photon Microscopy of Oxygen Consumption in the Brain
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批准号:9103239
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项目类别:
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资助金额:$45.48万
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财政年份:2015
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负责人:Sava Sakadzic
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依托单位:
Two-Photon Microscopy of Oxygen Consumption in the Brain
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批准号:9265146
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项目类别:
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资助金额:$45.75万
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财政年份:2015
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负责人:Sava Sakadzic
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依托单位:
国内基金
海外基金
层出镰刀菌氮代谢调控因子AreA 介导伏马菌素 FB1 生物合成的作用机理
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批准号:2021JJ40433
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项目类别:省市级项目
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资助金额:--
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批准年份:2021
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负责人:孙磊
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依托单位:
寄主诱导梢腐病菌AreA和CYP51基因沉默增强甘蔗抗病性机制解析
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批准号:32001603
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项目类别:青年科学基金项目
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资助金额:24.0万元
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批准年份:2020
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负责人:段真珍
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依托单位:
AREA国际经济模型的移植.改进和应用
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批准号:18870435
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项目类别:面上项目
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资助金额:2.0万元
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批准年份:1988
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负责人:史树中
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依托单位: