Three-Photon Microscope for High-Resolution Deep Tissue Imaging
Three-Photon Microscope for High-Resolution Deep Tissue Imaging
批准号:
10282619
负责人:
Sava Sakadzic
金额:
$59.99万
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
已结题
起止时间:
2021-08-15 至 2022-08-14
关键词:
ArchitectureAreaBiologicalBiomedical ResearchBlood VesselsBostonBrainCalcium SignalingCommunitiesDevelopmentDiseaseFundingGeneral HospitalsHealthHippocampus (Brain)HumanImageImaging technologyLasersMassachusettsMicroscopeMissionModificationNational Institute of Mental HealthPhotonsPhysiologic pulseResearchResearch PersonnelResolutionRodentStructureTechnologyTissue imagingTissuesUnited States National Institutes of Healthbioimagingclinical imagingimaging capabilitiesimaging modalityimaging systemin vivoinstrumentinstrumentationnoveloptical imagingpre-clinicalrelating to nervous systemresearch studythree photon microscopytwo photon microscopywhite matter
中文摘要
项目摘要
三光子显微镜(3 PM)是一种光学成像技术,能够在一个特定的环境中进行更深的成像。
散射生物组织比目前领先的标准双光子显微镜(2PM),同时提供
附加的对比度机制和增强的空间分辨率。这项技术的进步使之成为可能
长波长、可调谐、高脉冲能量飞秒激光器和2PM中的相应修改
设置,以适应一种新的激励制度。
作为这些进步的直接结果,啮齿动物大脑中的3 PM成像深度相对于
2PM成像深度-从~700 μm到超过1,200 μm。由于如此大的改进,我们预计
3 PM将允许结构的突破性研究(例如,血管、神经胶质和神经结构),
功能(例如,氧合、钙信号传导和细胞相互作用),在临床前体内研究中,
在以前难以接近的组织层,如皮质下白色物质和
海马体。本提案中列出的11个NIH资助的项目将从这些成像中受益匪浅
能力的这些项目涵盖了与NIMH使命相关的各种研究主题,包括
提高临床成像模式的定量性,更好地了解
参与各种大脑状况发展的机制。
Martinos生物医学成像中心和马萨诸塞州总医院组成了一个多样化的
研究人员的社区,其中许多人是由美国国立卫生研究院的支持资助,进行尖端的生物医学
这些研究将进一步推进我们对人类健康和疾病的基本理解。这一拟议
3 PM成像系统将代表波士顿地区(可能是东北部)的第一台同类仪器
US),该软件在成像核心中可用。这种尖端仪器将使我们的研究团体
大大加强与NIMH使命相关的各种研究,这也将使马蒂诺
中心继续致力于成为先进生物医学成像的领导者。
英文摘要
Project Summary
Three-photon microscopy (3PM) is an optical imaging technology that enables significantly deeper imaging in a
scattering biological tissue than the current leading standard two-photon microscopy (2PM), while providing
additional contrast mechanisms and enhancing spatial resolution. This technology is made possible by advances
in long-wavelength, tunable, high-pulse-energy femtosecond lasers and corresponding modifications in 2PM
setups to accommodate a novel excitation regime.
As a direct result of these advancements, 3PM imaging depth in rodent brain is greatly extended with respect to
2PM imaging depth—from ~700 μm to more than 1,200 μm. Due to such a large improvement, we anticipate
that 3PM will allow breakthrough studies of the structure (e.g., vascular, glial, and neural architecture) and
function (e.g., oxygenation, calcium signaling, and cellular interactions) in preclinical in vivo studies, with cellular-
size resolution in previously inaccessible tissue layers, such as the subcortical white matter and the
hippocampus. The 11 NIH-funded projects listed in this proposal will significantly benefit from these imaging
capabilities. These projects span a diverse range of research topics relevant to NIMH mission, including
improvement of the quantitativeness of the clinical imaging modalities and better understanding of the
mechanisms involved in development of various brain conditions.
The Martinos Center for Biomedical Imaging and Massachusetts General Hospital constitute a diverse
community of researchers, many of whom are funded with NIH support, performing cutting edge biomedical
research that will further advance our fundamental understanding of human health and disease. This proposed
3PM imaging system will represent the first instrument of its kind in the Boston area (and probably Northeast
US) that is available within an Imaging Core. This cutting-edge instrumentation will allow our research community
to significantly enhance various research studies relevant to NIMH mission, and it will also allow the Martinos
Center to continue prospering as a leader for advanced biomedical imaging.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Two-photon Imaging of Oxygen and Blood Flow in Retinal and Cerebral Vasculature
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批准号:10184187
-
项目类别:
-
资助金额:$189.72万
-
财政年份:2021
-
负责人:Sava Sakadzic
-
依托单位:
Two-Photon Microscopy of Oxygen Consumption in the Brain
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批准号:8985334
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项目类别:
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资助金额:$38.44万
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财政年份:2015
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负责人:Sava Sakadzic
-
依托单位:
Two-Photon Microscopy of Oxygen Consumption in the Brain
-
批准号:9103239
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项目类别:
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资助金额:$45.48万
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财政年份:2015
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负责人:Sava Sakadzic
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依托单位:
Two-Photon Microscopy of Oxygen Consumption in the Brain
-
批准号:9265146
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项目类别:
-
资助金额:$45.75万
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财政年份:2015
-
负责人:Sava Sakadzic
-
依托单位:
国内基金
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项目类别:省市级项目
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依托单位:
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批准年份:1988
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负责人:史树中
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依托单位: