Research Supplements to Promote Diversity

项目摘要 这个提案是为了申请一个补充补助金，以支持一个有才华的，少数民族博士。同学们， Frank DeLuna，开发一种新的无标记成像系统，用于药物毒性检测， Yong Ye在德克萨斯大学圣安东尼奥的实验室。药物毒性的准确分析 由于新发现的候选药物由于毒副作用而失败， 导致约30%的临床流失，导致巨大的经济损失， 医药行业。这些失败的主要原因之一可以归因于以下事实： 大多数动物模型都不能准确反映人类的生理机能。因此有 在进行药物毒性试验之前， 进行临床试验为此，至关重要的是，不仅要构建小的组织芯片， 很好地模拟人体组织的结构和功能，而且还可以连续监测 人体器官芯片模型在药物作用下的动态行为 延长期限。父R01项目（1R01GM126571）的总体目标是解决 通过监测人体器官芯片模型进行药物毒性测定的挑战性问题 利用自动化的、无标记的光学生物传感器系统， 敏感性和动力学分析的人类心脏组织模型，在响应于各种药物， 他们的微环境。根据本补充规定为学生提出的研究活动 格兰特是开发一种新的无标记成像系统，利用光子晶体生物传感器在 独特的检测模式，用于准确定量药物毒性引起的生物标志物。的 拟议的工作与父项目中的目标2有关，它还将为以下方面创造新的能力： 在传感器的渐逝场中直接对样品成像，用于同时监测 每个样品孔中的反射指数变化归因于由生物标志物分泌的生物标志物的结合事件。 在传感器表面上具有生物识别探针的芯片上的心脏模型。新设计将提供 一种良好的替代方法，用于药物毒性测定中生物标志物的多重检测。这 补助金将为少数民族学生提供一个极好的培训机会， 生物光子学的知识和必要的研究技能，以及提高他的其他 能力，如写作和网络技能，为他成功的学术生涯做好准备。

Project Summary This proposal is to apply for a supplement grant to support a talented, minority Ph.D. student, Frank DeLuna, to develop a new label-free imaging system for drug toxicity assays in Dr. Jing Yong Ye’s laboratory at the University of Texas at San Antonio. Accurate analysis of drug toxicities is in urgent demand as failures of newly discovered drug candidates due to toxic side effects have resulted in about 30% of clinical attrition, leading to enormous economic losses for the pharmaceutical industry. One of the main reasons for these failures can be attributed to the fact that most animal models fail to accurately reflect human physiology. Therefore, there is a significant interest in constructing human organ models for drug toxicity assays before proceeding with clinical trials. For that, it is critically important not only to construct small tissue chips that can well mimic the architecture and functionality of human tissues, but also to continuously monitor the dynamic behaviors of human organ-on-a-chip models in response to drugs in situ over an extended period. The overarching goal of the parent R01 project (1R01GM126571) is to address the challenging issues of drug toxicity assays by using a human organ-on-a-chip model monitored with an automated, label-free, optical biosensor system that allows for real-time, long-term, sensitive, and kinetic analyses of human cardiac tissue models in response to various drugs in their microenvironments. The proposed research activities for the student under this supplement grant is to develop a new label-free imaging system by utilizing a photonic crystal biosensor in a unique detection mode for accurate quantification of biomarkers due to drug toxicities. The proposed work is related to Aim 2 in the parent project, and it will also create a new capability for directly imaging samples in the evanescent field of the sensor for simultaneously monitoring reflective index changes in each sample well due to binding events of biomarkers secreted by a heart-on-a-chip model with biorecognition probes on the sensor surface. This new design will offer a good alternative approach for multiplex detection of biomarkers in drug toxicity assays. This supplement grant will offer an excellent training opportunity for the minority student to gain deep knowledge and necessary research skills in biophotonics as well as to improve his other capabilities, such as writing and networking skills, preparing him for a successful academic career.