Sample Processing and Immunoassay Research Core

项目总结 样品处理和免疫分析研究螺旋核心(核心C)服务于 RDRCC研究社区作为临床准备和存储的集中资源 衍生样本，用于对临床衍生的进行一系列标准和创新的分析 材料，并作为连接其他相关机构研究中心的渠道。几十年来， 这一核心通过专门针对疾病的卓越中心，促进了大型 从有良好血统的患者队列中获得的标本生物信息库。这个久负盛名的核心， 通过提供生物样品的获取、处理、存储和检索服务，已经成为一种 在实现翻译界面发现方面的关键合作伙伴，以及吸引 来自机构内外的越来越多的研究人员。在对这项研究的调查中 社区，这个核心被视为一个主要的资源，广泛地支持关于明确定义的学术 患者队列前瞻性地跟随，存储高质量的样本。高品质的 Core C提供的化验服务导致了大量同行评议的出版物、摘要 以及由调查员发起的拨款提案。核心中的化验能力在 人类风湿病队列中的通路验证。这项提议的目的是为了维持， 发展和发展这一至关重要的资源。我们增加了免疫的高维代谢分析 乔纳森·鲍威尔博士的细胞群和免疫效应器功能的分析。核心领导人已经 建立互动，以指导调查人员获得必要的机构专门知识和资源 提供单细胞分析、基于序列的免疫活性分析和高级数据 分析和解释。 核心C将由卡西奥拉-罗森和达拉博士执导。卡西奥拉-罗森博士带来了 几十年来在运营高效且预算稳定的研究核心方面的经验和严谨，以及 自身抗体的发现。Darrah博士最近担任了Flow Core的领导，并带来了 具有基于序列免疫分析的经验。安德拉德和古铁雷斯博士，他们都是 拥有多年与这个核心团队合作的经验，将提供额外的领导力。Dr。 鲍威尔领导了高维免疫新陈代谢分析。

PROJECT SUMMARY The Sample Processing and Immunoassay REsearch SPIRE Core (Core C) serves the RDRCC research community as a centralized resource for preparation and storage of clinically derived samples, for conducting a range of standard and innovative assays on clinically-derived material, and for serving as a conduit to other relevant Institutional research Cores. Over decades, this core has facilitated, through disease-specific Centers of Excellence, the generation of large specimen bio-repositories obtained from well-pedigreed patient cohorts. This well-established Core, by providing biospecimen acquisition, processing, storage, and retrieval services, has become a critical partner in enabling discovery at the translational interface, and in attracting the interest of a growing group of researchers from inside and outside the institution. In a survey of the research community, this Core was viewed as a major resource, broadly enabling scholarship on well-defined patient cohorts followed prospectively, with the storage of high-quality samples. The high-quality assay services provided by Core C have resulted in numerous peer-reviewed publications, abstracts and investigator-initiated grant proposals. The assay capabilities in the Core play a critical role in pathway validation in human rheumatic disease cohorts. The aims of this proposal are to sustain, grow and evolve this vital resource. We have added high-dimensional metabolic analysis of immune cell populations by Dr. Jonathan Powell, and assays of immune effector function. Core leaders have established interactions to guide investigators to the necessary institutional expertise and resources that provide single cell analysis, sequence-based assays of immune activity, and advanced data analysis and interpretation. Core C will be directed by Drs. Casciola-Rosen and Darrah. Dr. Casciola-Rosen brings decades of experience and rigor in running a highly effective and budget-stable research Core, and in autoantibody discovery. Dr. Darrah recently assumed leadership of the Flow Core, and also brings experience in sequence-based immune-based assays. Drs. Andrade and Gutierrez, both of whom have many years of experience working with this Core team, will provide additional leadership. Dr. Powell leads the high-dimensional immunometabolism analysis.