Sample Processing and Immunoassay Research Core

样品处理和免疫分析研究核心

• 批准号: 10487455
• 负责人: LIVIA A CASCIOLA-ROSEN
• 金额: $ 25.73万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-09 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10487455
• 关键词: ACE2; Applications Grants; Autoantibodies; Autoimmunity; Bar Codes; Base Sequence; Biological; Biological Assay; Biopsy; Budgets; COVID-19; Cataloging; Cell Separation; Cells; Clinical; Collaborations; Collection; Color; Communities; Complement Activation; Complex; Consultations; Coupled; Coupling; Credentialing; Custom; DNA; Data Analyses; Deposition; Diagnosis; Disease; Distant; Endothelium; Ensure; Environment; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Funding; Generations; Genetic Variation; Human; Human Biology; Immune; Immunoassay; Immunoglobulin M; Immunohistochemistry; Immunology procedure; In Vitro; Infrastructure; Institution; Knowledge; Laboratories; Leadership; Measurement; Metabolic; Modality; Molecular; Monitor; Pathogenicity; Pathway interactions; Patients; Peer Review; Peripheral Blood Mononuclear Cell; Phenotype; Plasma; Play; Population; Preparation; Prevention; Price; Process; Productivity; Publications; Quality Control; RNA; RNA library; Rare Diseases; Research; Research Personnel; Resources; Retrieval; Rheumatism; Rheumatology; Role; Running; Sampling; Scholarship; Services; Site; Specimen; Standardization; Study models; Surveys; Temperature; Testing; Time; Training; Validation; Whole Blood; Work; autoimmune rheumatologic disease; base; career development; clinical center; clinical phenotype; cohort; experience; genetic pedigree; high dimensionality; human disease; innovation; interest; member; mouse model; patient subsets; prospective; ranpirnase; recruit; repository; severe COVID-19; single cell analysis; synergism; tool; transcriptome sequencing

PROJECT SUMMARY The Sample Processing and Immunoassay REsearch SPIRE Core (Core C) serves the RDRCC research community as a centralized resource for preparation and storage of clinically derived samples, for conducting a range of standard and innovative assays on clinically-derived material, and for serving as a conduit to other relevant Institutional research Cores. Over decades, this core has facilitated, through disease-specific Centers of Excellence, the generation of large specimen bio-repositories obtained from well-pedigreed patient cohorts. This well-established Core, by providing biospecimen acquisition, processing, storage, and retrieval services, has become a critical partner in enabling discovery at the translational interface, and in attracting the interest of a growing group of researchers from inside and outside the institution. In a survey of the research community, this Core was viewed as a major resource, broadly enabling scholarship on well-defined patient cohorts followed prospectively, with the storage of high-quality samples. The high-quality assay services provided by Core C have resulted in numerous peer-reviewed publications, abstracts and investigator-initiated grant proposals. The assay capabilities in the Core play a critical role in pathway validation in human rheumatic disease cohorts. The aims of this proposal are to sustain, grow and evolve this vital resource. We have added high-dimensional metabolic analysis of immune cell populations by Dr. Jonathan Powell, and assays of immune effector function. Core leaders have established interactions to guide investigators to the necessary institutional expertise and resources that provide single cell analysis, sequence-based assays of immune activity, and advanced data analysis and interpretation. Core C will be directed by Drs. Casciola-Rosen and Darrah. Dr. Casciola-Rosen brings decades of experience and rigor in running a highly effective and budget-stable research Core, and in autoantibody discovery. Dr. Darrah recently assumed leadership of the Flow Core, and also brings experience in sequence-based immune-based assays. Drs. Andrade and Gutierrez, both of whom have many years of experience working with this Core team, will provide additional leadership. Dr. Powell leads the high-dimensional immunometabolism analysis.

项目摘要 样品处理和免疫分析研究SPIRE核心（核心C）服务于 RDRCC研究社区作为临床准备和储存的集中资源 衍生样品，用于对临床衍生样品进行一系列标准和创新检测 材料，并作为一个管道，以其他相关的机构研究核心。几十年来， 该核心通过疾病特异性卓越中心促进了大量 从血统良好的患者队列中获得的标本生物储存库。这是一个很好的核心， 通过提供生物标本采集、处理、储存和检索服务， 关键的合作伙伴，使发现在翻译接口，并在吸引的兴趣， 越来越多的研究人员从内部和外部的机构。在一项调查中， 社区，这一核心被视为一个主要的资源，广泛地使奖学金定义明确 患者队列前瞻性随访，储存高质量样本。高质量 核心C提供的分析服务已经产生了许多同行评审的出版物、摘要 和财政部发起的赠款提案。核心的分析能力在以下方面发挥着关键作用： 在人类风湿性疾病队列中的通路验证。这项建议的目的是维持， 发展和进化这一重要资源。我们增加了免疫的高维代谢分析， 细胞群，以及免疫效应功能测定。核心领导人有 建立互动机制，指导调查人员利用必要的机构专门知识和资源 提供单细胞分析、基于序列的免疫活性测定和高级数据 分析和解释。 核心C将由Casciola-Rosen博士和Darrah博士指导。卡肖拉-罗森医生 数十年的经验和严谨的运行一个高效和稳定的研究核心，并在 自身抗体发现Darrah博士最近担任了Flow Core的领导，并带来了 基于序列的免疫分析经验。安德拉德医生和古铁雷斯医生 我有多年与核心团队合作的经验，将提供额外的领导能力。博士 鲍威尔领导了高维免疫代谢分析。