Core B - Bioassay Core

核心 B - 生物测定核心

• 批准号: 8535518
• 负责人: LIVIA A CASCIOLA-ROSEN
• 金额: $ 20.02万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8535518
• 关键词: Antibodies; Applications Grants; Autoantibodies; Autoimmune Process; Back; Biological Assay; Biology; Biopsy; Cardiovascular system; Cataloging; Catalogs; Cells; Clinical; Collaborations; Collection; Complex; Controlled Environment; Coupled; Coupling; Custom; DNA; Diagnosis; Disease; Ensure; Environment; Enzyme-Linked Immunosorbent Assay; Failure; Flow Cytometry; Funding; Genetic Variation; Genome; Gerontology; Human; Human Biology; Human Subject Research; Hypersensitivity; Image; Immunohistochemistry; Immunology; In Vitro; Individual; Instruction; Investigation; Knowledge; Label; Laboratories; Lead; Leadership; Lung; Marketing; Molecular; Molecular Biology; Monitor; Neurosciences; Notification; Patients; Peer Review; Pharmacology; Plasma; Play; Population; Preparation; Price; Process; Productivity; Publications; Quality Control; RNA; Rare Diseases; Recruitment Activity; Research; Research Infrastructure; Research Personnel; Resources; Retrieval; Rheumatism; Role; Sampling; Scholarship; Science; Serum; Services; Standardization; Structure; System; Techniques; Technology; Temperature; Testing; Time; Training; Whole Blood; Work; abstracting; assay development; base; clinical phenotype; cohort; cost; design; experience; human disease; innovation; interest; member; molecular pathology; mouse model; novel; oncology; outreach; prevent; ranpirnase; sample collection; stem cell biology; tool

Answering important questions about human disease mechanism, diagnosis and therapy requires that molecular tools be coupled effectively to detailed clinical phenotyping, particularly in patients followed prospectively over time. Bioassay Core (Core B) is designed to serve the Research Base as a centralized resource for standardized preparation and storage of clinical samples, and for conducting a range of high quality, low-cost assays on clinically derived material. The latter will include both assays performed using commercial kits, as well as custom-designed assays. In the past 5 years, the Bioassay Core has proven its ability to serve the Research base by providing crucial biospecimens and assays for numerous peer reviewed publications, abstracts, and investigator-initiated grant proposals. The aim of this proposal is to continue growing this critical resource which fuels innovation, collaboration and facilitiates studies focused on a wide range of rheumatic diseases by researchers across the spectrum of investigation. Major services and assays that will be provided by the Bioassay Core include sample preparation (eg, serum, DNA, RNA, PBMCs), storage and retrieval, as well as ELISAs, autoantibody analyses and immunohistochemistry. The Core will also help to connect investigators to other appropriate institutional Cores, and participate in outreach activities. Core B will also provide training, management and quality control to staff in the Clinical Centers to ensure that best practice standards for processing, recordkeeping, and ensuring fidelity of labeling are uniformly applied, and to aid Investigators to take any custom assays established in the Core back to their own labs. The Core will be led by Dr. Livia Casciola-Rosen, who has been Director of this project for the past 3 years, and has many years experience in immunology and assay development. Additional leadership will be provided by Dr. Felipe Andrade and Dr. Laura Gutierrez. The availability of central systems to facilitate sample collection, processing, storage and assays has been critical to enabling scholarship on human rheumatic disease cohorts. The close coordination of this Core with the Administrative, Flow Cytometry and Human Subjects Research and Analytical Cores provides an extremely powerful matrix to maximize synergies in the environment.

要解决有关人类疾病机制、诊断和治疗的重要问题，需要将分子工具有效地与详细的临床表型相结合，特别是在随时间前瞻性随访的患者中。生物测定核心（核心B）旨在为研究基地提供服务，作为临床样本标准化制备和储存的集中资源，并对临床衍生材料进行一系列高质量、低成本的测定。后者将包括使用商业试剂盒进行的测定以及定制设计的测定。在过去的5年里，生物测定核心已经证明了它有能力为研究基地提供关键的生物标本和测定，为许多同行评审 出版物，摘要，和赞助商发起的赠款提案。这项建议的目的是 继续发展这一关键资源，推动创新，合作，促进研究人员在调查范围内对广泛的风湿性疾病进行研究。生物测定中心将提供的主要服务和测定包括样品制备（例如，血清、DNA、RNA、PBMC）、储存和检索以及ELISA、自身抗体分析和免疫组织化学。核心小组还将帮助将调查人员与其他适当的机构核心小组联系起来，并参与外联活动。核心B还将为临床 研究中心应确保统一应用处理、记录保存和确保标签保真度的最佳实践标准，并帮助研究者将核心研究中心建立的任何自定义检测方法带回自己的实验室。 核心将由Livia Casciola-Rosen博士领导，他在过去3年中一直担任该项目的负责人，并在免疫学和检测开发方面拥有多年经验。费利佩·安德拉德博士和劳拉·古铁雷斯博士将提供额外的领导。中央系统的可用性，以促进样本的收集，处理，储存和分析一直是至关重要的，使人类风湿性疾病队列的奖学金。该核心与行政、流式细胞术和人类受试者研究和分析核心的密切协调提供了一个极其强大的矩阵， 环境中的协同作用。