Bioassay Core

核心B生物化验核心旨在为研究基地提供集中的准备资源 和临床样本的存储，以及对临床衍生的 材料。在成立不到两年的时间里，生物检测核心已经证明了它的能力 为风湿科成员提供关键的化验服务，为两位同行评审 出版物，几篇摘要被接受在美国科学院发表 风湿病，以及三个最近提交的由研究人员发起的拨款提案。这项建议的目的是 是从这个强大的起点开始，为更多的调查人员提供更广泛的服务 师内外，调查自身免疫性风湿病的病因、诊断和治疗 疾病。虽然基础科学工作者和临床医生在历史上都在该部门表现出色，但近年来 该司见证了临床研究的飞跃，通过建立临床 SLE、硬皮病、炎症性关节炎、血管炎和最近的肌炎的卓越中心。 下一次飞跃将需要人力和物力资源在 在基础科学方面，生物分析核心代表着朝着这个方向迈出的关键一步。生物测评核心将 提供以下服务：样品制备和储存，包括从 全血和外周血单核细胞的制备和冷冻保存.用ELISA法和 多参数微珠分析；流式细胞术；免疫组织化学；自身抗体和其他 免疫学检测。核心将由詹姆斯·马奥尼博士领导，他自成立以来一直管理核心 成立以来，他在免疫学和化验开发方面拥有多年经验。额外的领导层将 由Mark Soloski博士和Livia Casciola-Rosen博士提供。生物分析核心的功能将提供 与基因组学/基因分型核心和临床卓越中心的关键协同作用，并将提供 为下一代发现自身免疫性风湿病提供了肥沃的环境。

The Core B Bioassay Core is designed to serve the Research Base as a centralized resource for preparation and storage of clinical samples, and for conducting a range of basic science assays on clinically derived material. In the less than two years since its founding, the Bioassay Core has already proven its ability to serve the members of the Division of Rheumatology, by providing crucial assay services for two peerreviewed publications, several abstracts accepted for presentation at the American College of Rheumatology, and three recently submitted investigator-initiated grant proposals. The aim of this proposal is to build from this strong beginning, providing a wider array of services to a larger number of investigators inside and outside the Division, investigating the causes, diagnosis, and treatment of autoimmune rheumatic disease. While both basic scienctists and clinicians have historically excelled in the Division, in recent years the Division has witnessed a leap forward in clinical research, through the establishment of the clinical Centers of Excellence in SLE, scleroderma, inflammatory arthritis, vasculitis, and most recently myositis. The next leap forward will require the same central coordination of human and physical resources on the basic science side, and the Bioassay Core represents a critical step in that direction. The Bioassay Core will provide the following services: sample preparation and storage, including RNA and DNA preparation from whole blood and PBMC preparation and cryopreservation; analysis of soluble molecules using ELISA and multiparameter bead-based assays; flow cytometry; immunohistochemistry; and autoantibodies and other immunological assays. The Core will be led by Dr. James Mahoney, who has managed the Core since its inception and has many years experience in immunology and assay development. Additional leadership will be provided by Drs. Mark Soloski and Livia Casciola-Rosen. The functions of the Bioassay Core will provide critical synergies with the Genomics/Genotyping Core and the clinical Centers of Excellence, and will provide a fertile environment for the next generation of discoveries in the autoimmune rheumatic diseases.