Bioassay Core

生物测定核心

• 批准号: 7666121
• 负责人: LIVIA A CASCIOLA-ROSEN
• 金额: $ 29.62万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7666121
• 关键词: American; Applications Grants; Arthritis; Autoantibodies; Autoimmune Process; Basic Science; Biological Assay; Biology; Biopsy; Cardiovascular system; Cataloging; Catalogs; Clinical; Clinical Research; Collection; Complex; Controlled Environment; Coupling; Cryopreservation; DNA; Diagnosis; Discipline; Disease; Environment; Enzyme-Linked Immunosorbent Assay; Failure; Flow Cytometry; Foundations; Funding; Future; Genetic Variation; Genome; Genomics; Genotype; Gerontology; Human; Human Biology; Hypersensitivity; Immunohistochemistry; Immunology; In Vitro; Investigation; Knowledge; Laboratories; Lead; Leadership; Lung; Marketing; Medical; Minor; Molecular Biology; Monitor; Myositis; Notification; Numbers; Patients; Peripheral Blood Mononuclear Cell; Phenotype; Play; Population; Preparation; Price; Process; Publications; RNA; Range; Rare Diseases; Rate; Research; Research Infrastructure; Research Personnel; Resources; Reunion Island; Rheumatism; Rheumatoid Arthritis; Rheumatology; Role; Sampling; Science; Scleroderma; Serum; Services; Side; Systemic Lupus Erythematosus; Technology; Telephone; Temperature; Testing; Training; Upper arm; Vasculitis; Whole Blood; Work; abstracting; assay development; base; clinical phenotype; college; cost; design; experience; human disease; interest; member; mouse model; next generation; prevent; programs; sample collection

The Core B Bioassay Core is designed to serve the Research Base as a centralized resource for preparation and storage of clinical samples, and for conducting a range of basic science assays on clinically derived material. In the less than two years since its founding, the Bioassay Core has already proven its ability to serve the members of the Division of Rheumatology, by providing crucial assay services for two peerreviewed publications, several abstracts accepted for presentation at the American College of Rheumatology, and three recently submitted investigator-initiated grant proposals. The aim of this proposal is to build from this strong beginning, providing a wider array of services to a larger number of investigators inside and outside the Division, investigating the causes, diagnosis, and treatment of autoimmune rheumatic disease. While both basic scienctists and clinicians have historically excelled in the Division, in recent years the Division has witnessed a leap forward in clinical research, through the establishment of the clinical Centers of Excellence in SLE, scleroderma, inflammatory arthritis, vasculitis, and most recently myositis. The next leap forward will require the same central coordination of human and physical resources on the basic science side, and the Bioassay Core represents a critical step in that direction. The Bioassay Core will provide the following services: sample preparation and storage, including RNA and DNA preparation from whole blood and PBMC preparation and cryopreservation; analysis of soluble molecules using ELISA and multiparameter bead-based assays; flow cytometry; immunohistochemistry; and autoantibodies and other immunological assays. The Core will be led by Dr. James Mahoney, who has managed the Core since its inception and has many years experience in immunology and assay development. Additional leadership will be provided by Drs. Mark Soloski and Livia Casciola-Rosen. The functions of the Bioassay Core will provide critical synergies with the Genomics/Genotyping Core and the clinical Centers of Excellence, and will provide a fertile environment for the next generation of discoveries in the autoimmune rheumatic diseases.

核心B生物测定核心旨在作为制备的集中资源服务于研究基地 临床样品的储存，并对临床衍生的 材料在成立不到两年的时间里，Bioassay Core已经证明了它的能力， 为流变学分部的成员服务，为两个同行评审的 出版物，几个摘要接受在美国大学的介绍， 流变学，和三个最近提交的赞助商发起的赠款提案。这项建议的目的是 是在这一良好开端的基础上，为更多的调查人员提供更广泛的服务 内外司，调查的原因，诊断和治疗自身免疫性风湿性关节炎 疾病虽然基础科学家和临床医生在历史上都在该部门表现出色，但近年来 该部门见证了临床研究的飞跃，通过建立临床 SLE、硬皮病、炎性关节炎、血管炎和最近的肌炎的卓越中心。 下一个飞跃将需要同样的人力和物力资源的中央协调， 基础科学方面，生物测定核心代表了朝着这个方向迈出的关键一步。生物测定核心将 提供以下服务：样本制备和储存，包括RNA和DNA制备 全血和PBMC制备和冷冻保存;使用ELISA分析可溶性分子， 多参数珠粒分析;流式细胞术;免疫组织化学;和自身抗体和其他 免疫学测定。核心将由詹姆斯·马奥尼博士领导，他自成立以来一直管理核心。 他在免疫学和检测开发方面拥有多年经验。更多的领导将 由Mark Soloski博士和Livia Casciola Rosen博士提供。生物测定核心的功能将提供 与基因组学/基因分型核心和临床卓越中心的关键协同作用，并将提供 为下一代自身免疫性风湿病的发现创造了肥沃的环境。