Integration of High Definition Display Technologies with Platinum Nanorod Microelectrodes for Large Scale in-vivo Recording and Stimulation

高清显示技术与铂纳米棒微电极的集成，用于大规模体内记录和刺激

• 批准号: 10293899
• 负责人: Shadi Dayeh
• 金额: $ 201.24万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10293899
• 关键词: Acute; Address; Aging; Animal Testing; Animals; Area; Boston; Brain; Brain Mapping; California; Cells; Chemicals; Chronic; Clinical; Devices; Electrocorticogram; Electrodes; Electronics; Electrophysiology (science); Elements; Engineering; Environment; Evoked Potentials; Film; Frequencies; Future; Gallium; Generations; Goals; Heating; Human; Implant; Indium; Infrastructure; Intervention; Limb structure; Mechanics; Medical; Microelectrodes; Modality; Motor Cortex; Motor Evoked Potentials; Mus; Muscle; Neurons; Neurosciences; Noise; Optics; Performance; Physiologic pulse; Platinum; Process; Property; Rattus; Research Personnel; Resolution; Resources; Rodent; Sensory; Side; Source; Techniques; Technology; Testing; Thinness; Tissues; Transistors; Universities; Validation; Vertebral column; Vibrissae; Work; Zinc Oxide; awake; biomaterial compatibility; clinical application; cognitive performance; cold temperature; density; design; electric impedance; experimental study; flexibility; implantation; in vivo; in vivo monitoring; innovation; interest; microstimulation; multimodality; nanorod; neurotechnology; new technology; novel; novel strategies; operation; optical imaging; pre-clinical; relating to nervous system; response; simulation; spatiotemporal; technology development; tool

ABSTRACT We propose to develop novel neurorecording devices using sequential thin-film transistors that are capable of recording and stimulating brain activity with thousands of channels using only 8 wires and to demonstrate broadband recordings with large area coverage in fully awake, chronically implanted mice performing a decision task. Our approach leverages the low temperature processing of the high mobility indium gallium zinc oxide (IGZO) thin film transistors (TFTs) on flexible substrates and uses chemically etched platinum nanorods (PtNRs), with nearly an ideal electrochemical interface for recording and stimulation that is of low impedance, stable/durable, and biocompatible, and which can be scaled to very tight pitches and high densities without compromising these properties. Our efforts are staged with device, circuit, and electrochemical benchtop testing, followed by in vivo acute and chronic recordings in rodents and compare the fidelity of the recordings across all frequencies with side-by-side integrated passive electrodes. In Aim 1, we will fabricate PtNR-IGZO multiplexing TFT arrays and perform comprehensive benchtop testing to validate sensitivity and stability by accelerated aging in the wet environment and validation of recording and stimulation in acute rat experiments. In Aim 2, we will scale the novel PtNR-sequential TFT (PtNR-SEQTFT) arrays to record/stimulate from 5041/100 contacts using only 8 wires and validate their operation in benchtop and acute rat experiments. In Aim 3, we will optimize the PtNR-SEQTFT for chronic implantation in mice and utilize two layouts: (1) Type I will have the TFTs located on top of the electrode grid as a necessary “preclinical” step toward an eventual clinical device (human recordings are beyond the current scope); (2) Type II will have the TFTs arranged on the periphery of the array making the electrode array area optically transparent. This device is targeted for basic neuroscience applications in awake, chronically implanted mice performing a decision task to demonstrate the ability of this novel technology to bridge single-cell neuronal activity to large-scale circuit phenomenon of brain waves and to cognitive performance. This project will enable a new generation of microelectrode arrays with superior spatiotemporal resolution to provide a panoramic view of the coordinated brain activity across multiple regions that produces function. It has potential to address fundamental neuroscience questions that require large scale recordings and to be advanced for future clinical applications. The technology is also extendable to depth electrodes and is compatible with complementary multimodal brain interrogation technologies. Our project builds around a true interdisciplinary integration of electrode interfaces and devices, circuit design, and neuroscience. We will advance and disseminate this technology leveraging collaborative ties among the participating investigators and extensive resources and infrastructure at University of California San Diego (UCSD) and Boston University (BU).

摘要 我们建议开发新的神经记录设备，使用顺序薄膜晶体管，能够 记录和刺激大脑活动的数千个通道，仅使用8根电线，并证明 在完全清醒的慢性植入小鼠中进行决策的大面积覆盖的宽带记录 任务我们的方法利用了高迁移率铟镓锌氧化物的低温处理 在柔性衬底上的IGZO（IGZO）薄膜晶体管（TFT），并使用化学蚀刻的铂纳米棒（PtNR）， 由于用于记录和刺激的几乎理想的电化学界面具有低阻抗， 稳定/耐用和生物相容的，并且可以缩放到非常紧凑的间距和高密度， 破坏这些特性。我们的努力是分阶段的设备，电路和电化学台式测试， 随后在啮齿动物中进行体内急性和慢性记录，并比较所有记录的保真度。 频率与并排集成无源电极。 在目标1中，我们将制造PtNR-IGZO复用TFT阵列，并进行全面的台架测试， 通过在潮湿环境中加速老化和记录验证来验证灵敏度和稳定性， 刺激急性大鼠实验。在目标2中，我们将缩放新型PtNR-Sequential TFT（PtNR-SEQTFT） 仅使用8根导线记录/刺激5041/100触点的阵列，并在工作台上验证其操作 和急性大鼠实验。在目标3中，我们将优化PtNR-SEQTFT用于小鼠中的慢性植入， 利用两种布局：（1）类型I将TFT位于电极网格顶部，作为必要的“临床前” 迈向最终临床设备的一步（人类记录超出了当前范围）;（2）II型将具有 布置在阵列外围的TFT使得电极阵列区域光学透明。这 该设备的目标是在清醒的基础神经科学应用，长期植入小鼠执行决策 任务是证明这种新技术能够将单细胞神经元活动连接到大规模电路 脑电波现象和认知表现。 该项目将使新一代微电极阵列具有上级时空分辨率， 提供了一个全景的协调大脑活动跨多个区域，产生功能。它有 解决需要大规模记录的基础神经科学问题的潜力， 用于未来的临床应用。该技术还可扩展到深度电极，并与 互补的多模式大脑审讯技术我们的项目围绕一个真正的跨学科 电极接口和设备的集成、电路设计和神经科学。我们将继续前进， 利用参与研究者之间的协作关系传播这项技术， 加州圣地亚哥大学（UCSD）和波士顿大学（BU）的资源和基础设施。