Discovery, Replication, and Validation of Biomarkers of the DASH Diet and Hypertension

DASH 饮食和高血压生物标志物的发现、复制和验证

• 批准号: 10295307
• 负责人: Casey Marie Rebholz
• 金额: $ 74.6万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10295307
• 关键词: Address; Adherence; Adult; Atherosclerosis Risk in Communities; Betaine; Biological; Biological Assay; Biological Markers; Biological Process; Biomarker of Dietary Intake; Biometry; Blood; Blood Pressure; Cardiac health; Cardiometabolic Disease; Cardiovascular Diseases; Cholesterol; Chronic Disease; Citrus; Clinical; Coffee; Cohort Studies; Collaborations; Communities; Complement; Consumption; DASH diet; Dairy Products; Data; Diet; Dietary Fiber; Dietary Intervention; Dietary Practices; Dietary intake; Disease Outcome; Ensure; Fatty acid glycerol esters; Fibrinogen; Food; Funding; Goals; Hypertension; Intake; Intervention Trial; Knowledge; Laboratories; Measurement; Measures; Mediating; Metabolic Pathway; Methodology; Methods; Nutrient; Nutritional Biochemistry; Outcome; Pathway interactions; Phase; Physiological; Population Heterogeneity; Prevention approach; Principal Investigator; Processed Meats; Proline; Proteins; Proteome; Proteomics; Public Health; Publishing; Randomized; Recording of previous events; Research; Research Design; Research Personnel; Risk; Sodium; Specimen; Techniques; Training; United States National Institutes of Health; Urine; Validation; Woman; Xanthines; base; biomarker panel; biomarker validation; blood pressure reduction; candidate marker; candidate validation; cardioprotection; cardiovascular disorder prevention; cardiovascular disorder risk; cardiovascular health; cohort; cost effective; dietary; dietary adherence; dietary control; feeding; follow-up; fruits and vegetables; hypertension prevention; improved; insight; member; men; metabolome; metabolomics; middle age; modifiable risk; multiple omics; novel marker; nutrition; repository; saturated fat; study population; trimethyloxamine

ABSTRACT Diet is a modifiable risk factor for one of the most serious threats to global public health: cardiovascular disease. The Dietary Approaches to Stop Hypertension (DASH) dietary pattern is one example of a high- quality diet that is high in fruits, vegetables, and low-fat dairy products and low in sodium, saturated fat, and cholesterol. The DASH diet is widely recognized as an effective approach for the prevention of hypertension, cardiovascular disease, and related cardiometabolic diseases. Existing biomarkers of dietary intake are few and represent single nutrients rather than a holistic dietary pattern. Metabolomics and proteomics are high- throughput methods for efficient measurement of compounds in biological specimens. The metabolome reflects the breakdown products of food that is consumed and can represent not only food items and their components but also dietary patterns. The proteome represents biological function and activity, which complements the metabolomic profile. We propose to conduct untargeted metabolomics and proteomics for the discovery of novel biomarkers of the DASH diet as well as biomarkers of DASH diet mediated blood pressure reduction. Findings will be replicated in two independent study populations and validated by using targeted, quantitative assays of candidate biomarkers. This cost-effective proposal leverages the existence of study data and biospecimens in the NHBLI BioLINCC repository as well as existing omics data. The study team is led by a K01-funded, early stage investigator who has been trained in metabolomics of diet and chronic disease outcomes. Study team members are established, senior investigators who have a history of collaboration with the Principal Investigator and complementary expertise in nutritional biochemistry, untargeted metabolomics and proteomics platforms, laboratory methodology, biostatistics, dietary intake, blood pressure, and deep knowledge of the study populations to be investigated. This proposed research will advance the current methods for assessing dietary intake and will provide insights for clinically-important, diet- modifiable metabolic pathways.

抽象的 饮食是对全球公共卫生最严重威胁之一的可修改风险因素：心血管 疾病。停止高血压（DASH）饮食方式的饮食方法是高度的一个例子 优质饮食含有水果，蔬菜和低脂乳制品，钠，饱和脂肪和低脂乳制品的饮食 胆固醇。破折号饮食被广泛认为是预防高血压的有效方法， 心血管疾病和相关的心脏代谢疾病。现有的饮食摄入生物标志物很少 并代表单一营养素，而不是整体饮食模式。代谢组学和蛋白质组学是高的 用于有效测量生物标本中化合物的吞吐量方法。代谢组 反映食用食物的细分产品，不仅可以代表食物及其 组件和饮食模式。蛋白质组代表生物学功能和活性， 补充代谢组分。我们建议对未靶向的代谢组学和蛋白质组学进行 发现仪表板饮食的新型生物标志物以及仪表板饮食介导的血液的生物标志物 减压。调查结果将在两个独立研究人群中复制，并通过使用 针对候选生物标志物的有针对性的定量测定。这个具有成本效益的建议利用了 在NHBLI BIOLINCC存储库以及现有的OMICS数据中研究数据和生物测量。研究 团队由K01资助的早期调查员领导，他接受了饮食和慢性代谢组学培训 疾病结果。建立了学习团队成员，高级研究人员的历史 与主要研究者和营养生物化学的补充专业知识合作， 未靶向的代谢组学和蛋白质组学平台，实验室方法论，生物统计学，饮食摄入，血液 压力和对研究人群的深入了解。这项拟议的研究将 推进当前评估饮食摄入量的方法，并将为临床重要的饮食提供见解 可修改的代谢途径。