The joint WCM-NYGC Center for Functional and Clinical Interpretation of Tumor Profiles

WCM-NYGC 肿瘤特征功能和临床解读联合中心

• 批准号: 10302065
• 负责人: Olivier Elemento
• 金额: $ 42.63万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-16 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10302065
• 关键词: Address; Algorithms; CLIA certified; Cells; Clinical; Code; Competence; Complex; Computational Biology; DNA Mutational Analysis; DNA Sequence Alteration; Data; Development; Doctor of Philosophy; Drug resistance; Evolution; Genome; Genome Data Analysis Network; Genomics; Graph; Institutes; Joints; Link; Malignant Neoplasms; Medical Oncologist; Medicine; Methods; Mutation; Neoplasm Metastasis; New York; Organoids; Outcome; Pattern; Pharmaceutical Preparations; Phase; Play; Preparation; Primary Neoplasm; Process; Publishing; Recurrence; Research Personnel; Sampling; Specific qualifier value; Structure; Testing; Untranslated RNA; Variant; Visualization software; algorithmic methodologies; analysis pipeline; analytical tool; cancer genomics; cell type; clinical phenotype; clinically relevant; computer infrastructure; computerized tools; data management; data portal; drug relapse; exome sequencing; experience; genome sequencing; genome-wide; innovation; novel; precision medicine; response; single cell sequencing; tool; transcriptome sequencing; transcriptomics; tumor; tumor heterogeneity; whole genome

The Weill Cornell Medicine-New York Genome Center (WCM-NYGC) Center for Functional and Clinical Interpretation of Tumor Profiles is submitted in response to RFA-CA-20-053. Continuing our involvement in the Genome Data Analysis Network (GDAN) over the past five years and leveraging novel algorithms and methods developed by our group, the Center will perform integrative analyses of coding and non-coding variants to unravel the function of specific classes of mutations and assess their clinical potential. As specified in the RFA, we have chosen to focus on two Core Competencies: (1) DNA Mutations (in coding and non-coding regions, somatic and/or germline) and (2) Copy Number / Purity Analysis ,with a focus on complex structural variants.Our team has developed novel algorithms and pipelines for the analysis of DNA mutations in coding and non-coding regions, characterization of complex structural variants, tumor evolution and linked-read sequencing. We have developed three Specific Aims. In Aim 1, we will perform systematic clinical and functional annotation of coding and non- coding mutations. This includes (1) clinical annotation of coding variants, (2) prioritization and functional annotation of non-coding variants (3) integration of transcriptomic analyses, such as cell type deconvolution of impure tumor samples to provide stromal context to somatic variants (4) correlation of variants with clinical phenotypes, including response to therapy. In Aim 2, we will analyze clinically relevant signatures of genome-wide somatic alteration patterns. We will utilize our state-of-the-art analytic tools for complex structural variant characterization and mutational topography to link (1) mutational processes and (2) cell-of-origin footprints to cancer outcome and drug response. We will also (3) adapt our cutting-edge genome graph visualization tools to build interactive data portals for browsing complex structural variation patterns in impure samples. In Aim 3, we will dentify and characterize variants that drive tumor evolution using multi-samples analysis. We will apply our state- of-the-art computational tools to study structural variant evolution across multiple tumor samples to (1) identify drivers of drug resistance and relapse in matched primary and recurrence/metastasis samples and (2) assess genomic divergence between primary tumors and matched tumor organoids.

威尔康奈尔医学-纽约约克基因组中心（WCM-NYGC）功能和临床研究中心 根据RFA-CA-20-053提交肿瘤特征解读。继续我们 在过去五年中参与基因组数据分析网络（GDAN），并利用 我们的团队开发的新算法和方法，该中心将进行综合分析， 编码和非编码变体，以解开特定类别突变的功能，并评估其 临床潜力正如RFA中所规定的，我们选择专注于两个核心竞争力：（1） DNA突变（编码区和非编码区，体细胞和/或生殖细胞）和（2）拷贝 Number / 纯度 分析 ，专注于复杂的结构变体。我们的团队开发了新颖的算法， 以及用于分析编码和非编码区DNA突变、表征 复杂的结构变异、肿瘤演变和连接阅读测序。我们开发了三个 具体目标。在目标1中，我们将进行编码和非编码的系统的临床和功能注释。 编码突变。这包括（1）编码变体的临床注释，（2）优先化和功能性注释，以及（3）编码变体的临床注释。 非编码变体的注释（3）转录组学分析的整合，例如细胞类型 不纯肿瘤样品的去卷积以提供基质背景与体细胞变体（4） 具有临床表型的变体，包括对治疗的反应。在目标2中，我们将分析临床 全基因组体细胞改变模式的相关特征。我们将利用我们最先进的 用于复杂结构变体表征和突变拓扑学的分析工具，以连接（1） 突变过程和（2）起源细胞足迹对癌症结果和药物反应的影响。我们将 还（3）调整我们的尖端基因组图可视化工具，以建立交互式数据门户， 浏览不纯样品中的复杂结构变异模式。在目标3中，我们将确定和 使用多样本分析来表征驱动肿瘤演变的变异。我们将应用我们的州- 最先进的计算工具来研究多个肿瘤样本的结构变异进化，以（1） 在匹配的原发和复发/转移样本中确定耐药性和复发的驱动因素 和（2）评估原发性肿瘤和匹配的肿瘤类器官之间的基因组差异。