Core C: Genomics & Bioinformatics Core

摘要 基因组学和生物信息学核心（GBC）将为项目提供集中的设施、设备和 全外显子组测序、转录组测序、单细胞RNAseq、血浆DNA 测序、ATAC-seq和ChIP-seq。所有研究项目都将使用GBC。GBC将由两名共同领导- 两个专业互补的私家侦探，Elemento博士和Mason博士. GBC将提供四种主要类型的 服务范围：1）临床样本和细胞的整合全外显子组测序和转录组测序 2）使用ERRBS、ChIPseq和ATAC对临床和研究样品进行整合的表观基因组分析。 seq，3）单细胞分析和血浆DNA监测，以及4）进行综合分析并促进 P01项目和核心之间的数据共享。核心合作PI及其实验室高度精通所有 提出的技术和分析，与一个合并的200和更多的出版物。尽可能 GBC将使用威尔康奈尔精密医学研究所的分析管道进行CLIA/CLEP 测定。

ABSTRACT The genomics and bioinformatics core (GBC) will provide the projects with centralized facilities, equipment and expertise for whole-exome sequencing, transcriptome sequencing, single cell RNAseq, plasma DNA sequencing, ATAC-seq and ChIP-seq. All research projects will use the GBC. The GBC will be led by two co- PIs with complementary expertise, Dr Elemento and Dr Mason. The GBC will provide four main types of services: 1) integrated whole-exome sequencing and transcriptome sequencing of clinical samples and cell lines, 2) integrated epigenomic profiling of clinical and research samples using ERRBS, ChIPseq and ATAC- seq, 3) single cell profiling and plasma DNA monitoring and 4) to perform integrative analysis and facilitate data sharing among P01 projects and cores. The Core co-PIs and their laboratories are highly proficient in all the proposed techniques and assays, with a combined 200 and more publications. Whenever possible, the GBC will use the analytical pipeline used at Weill Cornell’s Institute for Precision Medicine for its CLIA/CLEP assays.