Core-007

核心007

• 批准号: 10303169
• 负责人: CARYN LERMAN
• 金额: $ 81.11万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-01 至 2021-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10303169
• 关键词: Adult; Biological Assay; CLIA certified; Cancer Center; Cancer Center Support Grant; Center Core Grants; Characteristics; Chromatin; Code; DNA; DNA Methylation; DNA Sequence Alteration; DNA methylation profiling; Data; Environment; Epigenetic Process; Expression Profiling; Funding; Gene Expression; Genetic; Genetic Fingerprintings; Genetic Transcription; Genetic Variation; Genomics; Genotype; Goals; Health Sciences; Ions; Libraries; Los Angeles; Malignant - descriptor; Malignant Childhood Neoplasm; Malignant Neoplasms; Methylation; MicroRNAs; Molecular; Molecular Conformation; Pediatric Hospitals; Pediatric Oncology Group; Peer Review; Predisposition; Preparation; Process; Production; Protons; Research; Research Personnel; Role; Sampling; Services; Single Nucleotide Polymorphism; Site; Source; The Cancer Genome Atlas; Time; Tissues; University of Southern California Norris Cancer Center; Untranslated RNA; Variant; Workplace; base; bead chip; cost effective; density; epigenetic profiling; epigenetic variation; genetic analysis; genome-wide analysis; high throughput analysis; member; nano-string; new technology; programs; transcriptomics

The Molecular Genomics Core provides high-throughput analysis of genetic and epigenetic variations to researchers and clinicians of the USC Norris. The Core has two well-developed and differentiated sites: Health Sciences Campus (HSC) and Children’s Hospital Los Angeles (CHLA). The HSC site works with research samples, while the CHLA site specializes in providing services in a CLIA-compliant environment. The facility is led by Dr. David Van Den Berg, who has served as Core Director since 1999, with the support of two Co- Directors: Dr. Timothy Triche, CHLA, since 1999, and Dr. Charles Nicolet, HSC, since 2011. The Core continues to provide Cancer Center members with high-throughput biospecimen processing, DNA profiling of genetic variation, epigenetic profiling of DNA methylation and chromatin conformation, and expression profiling. At the time of the last CCSG renewal, the Molecular Genomics Core received a merit rating of excellent to outstanding. During the project period, the Core has acquired additional hardware platforms (Nanostring nCounter, Taqman Low Density Arrays, Ion Torrent PGM and Protons, Fluidigm BioMark, 2 x Illumina MiSeq and 2 x Illumina NextSeq500) to supplement existing capabilities for analysis of genetic variation, epigenetic variation and gene expression. It served as the only data production site for methylation assays for The Cancer Genome Atlas (TCGA), one of only a few sites processing the HumanOncoArray beadchip the OncoChip Consortium, and one of the largest Illumina single nucleotide polymorphism (SNP) and DNA methylation array production sites in the US. The facility has also supported the NCI Children’s Oncology Group (COG) and NCI funded TARGET program, which is profiling childhood cancer akin to TCGA’s role in adult cancer. In FY 2013-2014, 101 Cancer Center members (48% of users), of which 80 were peer-reviewed funded (38% of users) from seven Research Programs used the Molecular Genomics Core to accomplish their research objectives. The Core will continue to utilize expertise in high-throughput platforms to provide cost-effective options from single base to genome-wide analysis. The Core will continue to identify new technologies and services that may be offered to advance USC Norris research and to evaluate existing services for costeffectiveness and value added.

分子基因组学核心提供遗传和表观遗传变异的高通量分析， 南加州大学诺里斯分校的研究人员和临床医生。核心有两个发展良好和差异化的网站： 科学校园（HSC）和儿童医院洛杉矶（CHLA）。HSC网站与研究合作 示例，而CHLA网站专门提供符合CLIA的环境中的服务。该设施是 由大卫货车登贝格博士，谁一直担任核心主任自1999年以来，在两个共同支持， 主任：Timothy Triche博士，CHLA，自1999年起，Charles尼科莱博士，HSC，自2011年起。核心 继续为癌症中心成员提供高通量生物标本处理， 遗传变异，DNA甲基化和染色质构象的表观遗传分析，以及表达 侧写 在上一次CCSG更新时，分子基因组学核心获得了优秀的优点评级， 非常出色。在项目期间，Core获得了额外的硬件平台（Nanostring nCounter，Taqman Low Density Arrays，Ion Torrent PGM and Protons，Fluidigm BioMark，2 x Illumina MiSeq 和2个Illumina NextSeq500），以补充现有的遗传变异、表观遗传 变异和基因表达。它是癌症甲基化检测的唯一数据生成站点。 基因组图谱（TCGA），处理HumanOncoArray微珠芯片的少数几个位点之一 和Illumina最大的单核苷酸多态性（SNP）和DNA甲基化阵列之一 在美国的生产基地。该设施还支持NCI儿童肿瘤学小组（COG）和NCI 资助的目标计划，这是描绘儿童癌症类似于TCGA的作用，在成人癌症。 在2013 - 2014财年，101名癌症中心成员（48%的用户），其中80名是同行评审资助的（38%） 的用户）使用分子基因组学核心来完成他们的研究 目标.核心将继续利用高通量平台的专业知识， 从单碱基分析到全基因组分析。核心将继续识别新技术， 可以提供的服务，以推进南加州大学诺里斯分校的研究，并评估现有的服务的成本效益 和增值。