Integrated Metagenomic and Metabolomic Core

综合宏基因组和代谢组核心

• 批准号: 10304048
• 负责人: SHIRISH S BARVE
• 金额: $ 37.34万
• 依托单位: VANDERBILT UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-10 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10304048
• 关键词: AIDS/HIV problem; Affect; Aging; Alcohol consumption; Alcohols; Bile Acids; Bioinformatics; Biological Specimen Banks; Boston; Cardiovascular Diseases; Cessation of life; Clinical; Cohort Studies; Collaborations; Communities; Complement; Computational algorithm; Data; Data Analyses; Development; Ensure; Equipment and supply inventories; Evaluation; Fostering; Generations; Genes; Goals; Gold; HIV; HIV Infections; Heavy Drinking; Human Resources; Infrastructure; Institutes; Institution; Letters; Logistics; Longitudinal Studies; Mass Fragmentography; Medicine; Metagenomics; Methods; Molecular; Monitor; National Cancer Institute; National Institute on Alcohol Abuse and Alcoholism; Nomenclature; Outcome; Output; Participant; Plasma; Preparation; Probiotics; Procedures; Publishing; Quality Control; RNA; Randomized Clinical Trials; Recording of previous events; Research Design; Research Personnel; Research Project Grants; Resources; Role; Russia; Sampling; Sampling Studies; Shipping; Ships; Shotguns; Site; Specimen; Standardization; Taxon; Techniques; Therapeutic; Time; Uganda; Universities; Veterans; Volatile Fatty Acids; Work; alcohol research; base; biobank; clinical care; cohort; college; comorbidity; data management; data mining; data repository; data sharing; data standards; dysbiosis; efficacy evaluation; gut bacteria; gut dysbiosis; gut microbiota; host microbiota; improved; innovation; metabolome; metabolomics; metagenomic sequencing; microbial; microbiome; microbiome research; microbiota; probiotic supplementation; programs; rRNA Genes; sample collection; trimethyloxamine; whole genome

The Integrated Metagenomics and Metabolomics Core (IMMC) will provide a platform that interconnects Projects 1 and 2. Specifically, IMMC will provide high-quality, metagenomics and targeted metabolomics data, to be integrated with the probiotics randomized clinical trial and clinical cohort outcomes for Projects 1 and 2, respectively. The IMMC has already acquired relevant metagenomics and metabolomics data (see Preliminary data sections E1.2 and F.1.A.2) that supports the experimental rationale and study design for Projects 1 and 2. To ensure optimal scientific output from the study samples, the IMMC has implemented standardized procedures for collection of samples from Projects 1 and 2 study sites, and established logistics to coordinate shipment of samples for analysis, manage inventory, and enable tracking of chain of custody. For Project 1, IMMC - sub-core 1 will conduct state of the art metagenomics analysis of fecal samples collected from study participants receiving probiotics supplementation, to profile the changes in the gut microbial communities. The culture- independent molecular methods will consist of 16S rRNA gene and whole genome shotgun (WGS) metagenomic sequencing that provide the most comprehensive microbiota profiling. Rigorous sequence data analysis will utilize a set of advanced computational algorithms, according to taxon- based or function-based data matrices. IMMC- sub-core 2 will conduct targeted metabolomics analysis of plasma/serum (Projects 1 and 2) to monitor host and gut-microbiota metabolomes. The IMMC will collaborate with Projects to develop meaningful study designs, implement effective sample collection and storage strategies, generate high-quality metagenomics and targeted metabolomics data, computationally summarize these data and support interpretation of the results. It will implement high-quality techniques, along with quality control standards, that produce meaningful results in a timely fashion. The IMMC will also standardize data generation methods across studies to enable integration across Projects and uncover robust discoveries. To accomplish these goals the IMMC has established the following aims: 1. Employ metagenomic approaches to generate and analytically summarize project microbiome data. 2. Perform targeted metabolomics analyses to profile plasma/serum metabolites. 3. Manage specimen and data biorepository and facilitate transfer between research Projects.

整合宏基因组学和代谢组学核心（IMMC）将提供一个相互连接的平台