Integrated Metagenomic and Metabolomic Core

整合宏基因组学和代谢组学核心（IMMC）将提供一个平台， 项目1和2。具体来说，IMMC将提供高质量的宏基因组学和靶向代谢组学数据， 与益生菌随机临床试验和项目1和2的临床队列结果相结合， 分别IMMC已经获得了相关的宏基因组学和代谢组学数据（见初步报告 数据第E1.2和F.1.A.2节），支持项目1和2的实验原理和研究设计。 为了确保研究样本的最佳科学产出，IMMC实施了标准化的 从项目1和2研究中心收集样本的程序，以及协调的既定后勤工作 运送样品进行分析、管理库存并跟踪监管链。 对于项目1，IMMC -子核心1将对粪便样本进行最先进的宏基因组学分析 从接受益生菌补充剂的研究参与者中收集，以描述肠道的变化 微生物群落不依赖培养的分子生物学方法将包括16 SrRNA基因和全基因组测序。 基因组鸟枪法（WGS）宏基因组测序提供了最全面的微生物群分析。 严格的序列数据分析将利用一套先进的计算算法，根据分类- 基于或基于函数的数据矩阵。IMMC-子核心2将进行针对性的代谢组学分析， 血浆/血清（项目1和2），以监测宿主和肠道微生物群代谢组。 IMMC将与项目合作，开发有意义的研究设计，实施有效的样本 收集和存储策略，生成高质量的宏基因组学和靶向代谢组学数据， 通过计算总结这些数据并支持对结果的解释。将实施高质量 技术，沿着质量控制标准，及时产生有意义的结果。IMMC 还将标准化跨研究的数据生成方法，以实现跨项目的集成， 发现强大的发现。 为了实现这些目标，IMMC制定了以下目标： 1.采用宏基因组方法生成和分析总结项目微生物组数据。 2.进行靶向代谢组学分析，以分析血浆/血清代谢物。 3.管理标本和数据生物库，促进研究项目之间的转移。

The Integrated Metagenomics and Metabolomics Core (IMMC) will provide a platform that interconnects Projects 1 and 2. Specifically, IMMC will provide high-quality, metagenomics and targeted metabolomics data, to be integrated with the probiotics randomized clinical trial and clinical cohort outcomes for Projects 1 and 2, respectively. The IMMC has already acquired relevant metagenomics and metabolomics data (see Preliminary data sections E1.2 and F.1.A.2) that supports the experimental rationale and study design for Projects 1 and 2. To ensure optimal scientific output from the study samples, the IMMC has implemented standardized procedures for collection of samples from Projects 1 and 2 study sites, and established logistics to coordinate shipment of samples for analysis, manage inventory, and enable tracking of chain of custody. For Project 1, IMMC - sub-core 1 will conduct state of the art metagenomics analysis of fecal samples collected from study participants receiving probiotics supplementation, to profile the changes in the gut microbial communities. The culture- independent molecular methods will consist of 16S rRNA gene and whole genome shotgun (WGS) metagenomic sequencing that provide the most comprehensive microbiota profiling. Rigorous sequence data analysis will utilize a set of advanced computational algorithms, according to taxon- based or function-based data matrices. IMMC- sub-core 2 will conduct targeted metabolomics analysis of plasma/serum (Projects 1 and 2) to monitor host and gut-microbiota metabolomes. The IMMC will collaborate with Projects to develop meaningful study designs, implement effective sample collection and storage strategies, generate high-quality metagenomics and targeted metabolomics data, computationally summarize these data and support interpretation of the results. It will implement high-quality techniques, along with quality control standards, that produce meaningful results in a timely fashion. The IMMC will also standardize data generation methods across studies to enable integration across Projects and uncover robust discoveries. To accomplish these goals the IMMC has established the following aims: 1. Employ metagenomic approaches to generate and analytically summarize project microbiome data. 2. Perform targeted metabolomics analyses to profile plasma/serum metabolites. 3. Manage specimen and data biorepository and facilitate transfer between research Projects.