Life time imaging with pulsed x-ray based x-ray luminescence computed tomography

摘要 寿命成像，如荧光寿命成像，能够提供广泛的生理信息 在细胞或组织中。荧光寿命成像主要应用于表面目标或可以 通过光学导引到达的。对于深部目标，荧光寿命成像的空间分辨率低， 由于强烈的光学散射，图像质量较差。在这个提案中，我们将开发一种独特的、新颖的生物光子学 成像模式，时间域x射线发光计算机断层扫描(TdXLCT)，它结合了空间 X射线成像的分辨率与光学成像的高检测灵敏度。TdXLCT将是一个新的 探索深部目标寿命成像的方法。在tdxlct中，一束超细脉冲x射线束将激发 用灵敏的光电倍增管测量深部靶中的纳米荧光粉和发射的光子。 模块。 在这份行政补充提案中，受资助的研究生将建立终生 样品测量系统，评估不同环境下的寿命，以及合成和 表征具有更好发射效率的x射线可激发纳米荧光粉。此外，他还将为 首次研制了tdXLCT系统，并通过体模成像实验对其性能进行了评价。 拟议的tdxlct将探索x射线作为分子成像工具和手段的适用性。 为了获得关于深层组织微环境的物理化学特征的信息(例如 氧合水平)。建议的tdxlct将引起许多生物研究人员在以下领域的兴趣 纳米医学和癌症治疗，因为它有可能克服与 放射性标记(有限的半衰期)和荧光标记(深层空间分辨率较低)。 与父母助学金的关系：这项建议与父母助学金密切相关。他们两人都在探索 X射线发光成像技术的应用。然而，家长拨款的目的是探索高空间 连续波域的XLCT分辨率成像。这项建议旨在探索其在 时间域中的脉冲x射线发光。这项提议还将利用从 家长助学金。

SUMMARY Lifetime imaging, such as fluorescence lifetime imaging, is able to provide extensive physiological information in cells or tissues. Fluorescence lifetime imaging is mainly applied to superficial targets or objects that can be reached by an optical guidance. For deep targets, fluorescence lifetime imaging suffers low spatial resolution and poor image quality due to strong optical scattering. In this proposal, we will develop a unique, novel biophotonics imaging modality, time domain x-ray luminescence computed tomography (tdXLCT), which combines the spatial resolution of x-ray imaging with the high detection sensitivity of optical imaging. The tdXLCT will be a new approach to explore lifetime imaging in deep targets. In the tdXLCT, an ultrafine pulsed x-ray beam will excite nanophosphors in deep targets and the emitted photons will be measured with a sensitive photomultiplier tube module. In this administrative supplement proposal, the supported graduate student will build a lifetime measurement system for samples, evaluate the lifetime in different environments, as well as synthesize and characterize x-ray excitable nanophosphors with better emission efficiency. Furthermore, he will build, for the first time, a tdXLCT system and evaluate its performance with phantom imaging experiments. The proposed tdXLCT will explore the applicability of x-ray as a molecular imaging tool and as a means to obtain information on the physiochemical characteristics of microenvironments in deep tissues (e.g. oxygenation level). The proposed tdXLCT will be of interest to many biological researchers in the fields of nanomedicine and cancer therapy because it has the potential to overcome limitations associated with radiolabeling (limited half-life) and fluorescence tagging (poor spatial resolution at depths). Relationship to the parent grant: This proposal is closely related to the parent grant. Both of them explore the application of x-ray luminescence imaging. However, the parent grant aims to explore the high spatial resolution XLCT imaging in the continue wave (CW) domain. This proposal aims to explore the applications of pulsed x-ray luminescence in time domain. This proposal will also utilize the synthesized nanoparticles from the parent grant.