Molecular epidemiology and serology of malaria under shifting agricultural practices in Africa

非洲农业实践转变下疟疾的分子流行病学和血清学

• 批准号: 10308046
• 负责人: Elizabeth Anne Hemming-Schroeder
• 金额: $ 3.83万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-12-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10308046
• 关键词: Accounting; Affect; Africa; Africa South of the Sahara; African; Agriculture; Antibody Response; Antigens; Area; Bioinformatics; Biological Markers; Blood; Breeding; Cessation of life; Chronic; Clinic; Clinical; Clinical Research; Country; Coupled; Cross-Sectional Studies; Culicidae; Data; Detection; Development; Disease Surveillance; Drug resistance; Environment; Epidemiology; Faculty; Famines; Farm; Fellowship; Funding; Future; Genetic; Genetic Markers; Genetic Structures; Genetic Variation; Genotype; Goals; Habitats; Health; Hospitals; Human; Hunger; Immunologic Techniques; Immunology; Incidence; Infection; Interdisciplinary Study; International; Investments; Irrigation; Kenya; Knowledge; Lead; Leadership; Longitudinal cohort study; Malaria; Malnutrition; Measures; Mentorship; Microsatellite Repeats; Migrant; Modeling; Molecular Epidemiology; Monitor; Movement; Outcome; Parasite resistance; Parasites; Plasma; Plasmodium falciparum; Population; Population Genetics; Population Growth; Population Heterogeneity; Positioning Attribute; Predisposition; Public Health; Questionnaires; Recording of previous events; Research; Research Activity; Research Personnel; Research Training; Resource Development; Risk; Salivary Proteins; Scheme; Secure; Serology; Site; Sporozoites; Testing; Time; Training; Travel; Universities; Ursidae Family; Water; Work; World Health Organization; burden of illness; career; combat; deep sequencing; design; epidemiology study; experience; exposed human population; food insecurity; food security; genetic makeup; genomic data; global health; human migration; improved; insight; land cover; land use; malaria transmission; next generation sequencing; population genetic structure; population movement; professor; programs; remote sensing; serological marker; skills; spatiotemporal; vector

Project Summary This is an application for a F32 fellowship for Dr. Elizabeth Hemming-Schroeder, a Postdoctoral Scholar proposing to train under the sponsorship of Dr. James Kazura, Professor of Global Health at Case Western Reserve University. The overall goal for this fellowship is for Dr. Hemming-Schroeder to cultivate her skills to become a future independent investigator in the field of malaria epidemiology. Specifically, this F32 fellowship will provide Dr. Hemming-Schroeder with the necessary support to achieve the following goals: 1) Design epidemiological studies to test hypotheses related to malaria transmission and control; 2) Analyze large sets of genomic data using bioinformatics; 3) Use immunological techniques to study malaria epidemiology; and 4) Develop leadership and professional skills to lead an interdisciplinary research team. To combat food insecurity and meet increasing water demands under rapid population growth, water resource development projects are anticipated to rapidly increase in sub-Saharan Africa. The impacts that these water resource development projects will have on malaria risk remain poorly understood. This project will focus on assessing the impacts of shifting agricultural practices and human migration resulting from irrigation projects on parasite population genetics and malaria epidemiology. In newly irrigated and non-irrigated areas of Kenya, Dr. Hemming-Schroeder will monitor land use and population movement and use cross-sectional surveys, a longitudinal cohort study, and passive case surveillance to monitor changes in parasite populations in local residents and migrant laborers and malaria incidence in the areas over time. In Aim 1, Dr. Hemming-Schroeder will examine the impacts of shifting agricultural practices on parasite population genetics and diversity through genotyping of microsatellite and drug resistance markers, as well as amplicon next-generation sequencing and bioinformatic analysis. In Aim 2, Dr. Hemming-Schroeder will determine the impacts of shifting agricultural practices on symptomatic disease burden, parasite exposure history, and infection complexity through passive case detection in local health centers and hospitals, serology for biomarkers of parasite exposure, bioinformatic analysis of deep sequences, and mixed effects model analysis. This research will help to improve our understanding of the impacts water resource development projects have on malaria epidemiology and population genetics, which is critically important to being able to maintain effective malaria control programs. Data from this research will serve as preliminary data for a K22 or K99 application in the final year of the F32 fellowship. Through the proposed research activities and training plan, Dr. Hemming-Schroeder will gain skills in epidemiology, bioinformatics, immunology, and leadership and professional skills, which will help her to gain independent funding and prepare her for a faculty career leading an interdisciplinary research program.

项目概要 这是博士后学者 Elizabeth Hemming-Schroeder 博士的 F32 奖学金申请 提议在 Case Western 全球健康教授 James Kazura 博士的赞助下进行培训 储备大学。该奖学金的总体目标是让海明-施罗德博士培养她的技能 成为疟疾流行病学领域未来的独立研究者。具体来说，这个F32奖学金 将为 Hemming-Schroeder 博士提供必要的支持，以实现以下目标： 1) 设计 流行病学研究，以检验与疟疾传播和控制有关的假设； 2) 分析大量数据 使用生物信息学的基因组数据； 3）利用免疫学技术研究疟疾流行病学；和 4) 培养领导跨学科研究团队的领导力和专业技能。应对粮食不安全 为了满足人口快速增长下日益增长的用水需求，水资源开发项目 预计撒哈拉以南非洲地区将迅速增加。水资源开发的影响 项目对疟疾风险的影响仍知之甚少。该项目将重点评估 灌溉项目对寄生虫种群造成的农业实践转变和人类迁徙 遗传学和疟疾流行病学。在肯尼亚新灌溉和非灌溉地区，Hemming-Schroeder 博士 将监测土地利用和人口流动，并使用横断面调查、纵向队列研究和 被动病例监测，监测当地居民和外来务工人员寄生虫种群的变化， 随着时间的推移，这些地区的疟疾发病率。在目标 1 中，Hemming-Schroeder 博士将研究转移的影响 通过微卫星和药物基因分型研究寄生虫种群遗传学和多样性的农业实践 抗性标记，以及扩增子下一代测序和生物信息学分析。在目标 2 中，Dr. 海明-施罗德将确定农业实践转变对症状性疾病负担的影响， 通过当地卫生中心和医院的被动病例检测来了解寄生虫暴露史和感染复杂性 医院、寄生虫暴露生物标志物的血清学、深层序列的生物信息分析以及混合 效应模型分析。这项研究将有助于提高我们对水资源影响的了解 发展项目涉及疟疾流行病学和群体遗传学，这对于 能够维持有效的疟疾控制计划。这项研究的数据将作为初步数据 F32 奖学金最后一年的 K22 或 K99 申请。通过拟议的研究活动和 培训计划中，Hemming-Schroeder 博士将获得流行病学、生物信息学、免疫学和 领导力和专业技能，这将帮助她获得独立的资助，并为她成为一名教师做好准备 领导跨学科研究项目的职业生涯。

项目成果

Widespread multiple insecticide resistance in the major dengue vector Aedes albopictus in Hainan Province, China.

中国海南省主要登革热媒介白纹伊蚊普遍存在多种杀虫剂抗药性。

• DOI: 10.1002/ps.6222
• 发表时间: 2021-04
• 期刊: Pest management science
• 影响因子: 4.1
• 作者: Li Y;Zhou G;Zhong D;Wang X;Hemming-Schroeder E;David RE;Lee MC;Zhong S;Yi G;Liu Z;Cui G;Yan G
• 通讯作者: Yan G