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Cellular Mechanisms in Fetal Alcohol Spectrum Disorders

胎儿酒精谱系疾病的细胞机制

基本信息

批准号：
10308057
负责人：
Scott Parnell
金额：
$ 34.56万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-12-15 至 2023-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10308057
关键词：
Address Attenuated Brain Cell Cycle Cell Proliferation Cell physiology Cells Child Cilia Clinical Research Confocal Microscopy Congenital Abnormality Data Defect Development Disease Dorsal Down-Regulation Dysmorphology Ethanol Event Eye Face Fetal Alcohol Exposure Fetal Alcohol Spectrum Disorder Fetus Foundations Functional disorder Future Gene Expression Profiling Genes Genetic Goals Growth Hair Holoprosencephaly Imaging Techniques Immunohistochemistry In Situ Hybridization Intervention Joubert syndrome Lead Literature Malignant Neoplasms Mediating Molecular Molecular and Cellular Biology Morphology Neural tube Orbital separation excessive Organ Organelles Pathogenesis Pathogenicity Pathology Pattern Phenocopy Post-Translational Protein Processing Pregnancy Prevention Prosencephalon Quantitative Reverse Transcriptase PCR Research Role SHH gene Signal Pathway Signal Transduction Sonic Hedgehog Pathway Structure Syndrome Teratogenic effects Testing Therapeutic Studies Time Tubulin Work addiction alcohol effect alcohol exposure alcohol research base brain abnormalities ciliopathy cilium biogenesis design experimental study gastrulation insight morphogens mouse model novel smoothened signaling pathway teratogenesis transcriptome sequencing transcriptomics

项目摘要

Many of the structural and functional abnormalities associated with Fetal Alcohol Spectrum Disorders (FASD) have been uncovered, yet major gaps remain in our understanding of the associated pathogenesis and mechanisms. For example, it is well known that ethanol exposure during gastrulation results in the classic hypoteloric FAS face and midline forebrain dysgenesis; yet, exposure just slightly later, during neurulation, induces expanded midline brain structures and hypertelorism. Interestingly, these abnormalities resemble (phenocopy) those of many genetic ciliopathies, such as Joubert syndrome. The central pathogenic mechanism of ciliopathies is a perturbation of the structure and/or function of primary cilia, hair-like organelles found on most cells that integrate extra- and intra-cellular signals. The proposed research tests the overall novel hypothesis that neurulation-stage ethanol exposure induces a “transient ciliopathy” (i.e., a temporary disruption of primary cilia function) that is the basic cellular mechanism for the expansion of midline brain structures and hyperteloric dysmorphologies. The proposed experiments are designed to meet the following integrated specific aims. Aim 1 will define the direct effects of early prenatal ethanol exposure on primary cilia structure and function. For this, confocal microscopy and immunohistochemistry will be used to examine the effects of ethanol on primary cilia number while gene expression assays will be used to assess cilia function. It is hypothesized that ethanol exposure causes abnormal ciliary number and/or function, reducing activation of the Shh signaling pathway. Aim 2 will characterize the secondary cellular pathogenic events in the neural tube resulting from an ethanol-induced transient ciliopathy. The experiments in this aim will test the hypothesis that the ethanol-induced transient ciliopathy and subsequent down-regulation of the Shh pathway will decrease downstream cell proliferation genes in the ventral neural tube and expand morphogen gradients that pattern the dorsal neural tube. Following ethanol exposure, genes with known roles in cell proliferation will be assessed using qRT-PCR and the gradients of ventral and dorsal morphogens will be assessed using in situ hybridization. These data will help to determine the precise mechanisms by which ethanol alters development. Aim 3 is to determine the primary cellular mechanistic events underlying an ethanol-induced transient ciliopathy. This final Aim will use RNA-seq to determine in an unbiased manner how ethanol disrupts normal ciliogenesis by examining the total transcriptomic profile at several time points immediately following ethanol exposure. We hypothesize that ethanol will alter key ciliogenesis genes; however, using this non-biased approach will aid in identifying other potential changes. Finally, we test the alternative/complementary hypothesis that ethanol alters tubulin post-translational modification, thereby disrupting normal cilia stability and function. Together, these novel experiments will provide fundamental insights into the pathogenic mechanisms underlying the effects of ethanol exposure during development, and propel alcohol research into new primary ciliary-related studies.

许多与胎儿酒精谱系障碍（FASD）相关的结构和功能异常