Age-dependent effects on microglia-mediated control of neuronal activity

小胶质细胞介导的神经元活动控制的年龄依赖性影响

• 批准号: 10314367
• 负责人: Philip Henry Hwang
• 金额: $ 4.44万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-12-01 至 2025-11-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10314367
• 关键词: Age; dependent; effects; microglia; mediated

Project Summary/Abstract This proposal addresses a novel microglia-controlled neuronal activity feedback mechanism and its potential contribution to aging-dependent neuropathology. Aging in mice and humans is associated with alterations to neuronal circuit excitability and function, increased seizure susceptibility, and neurodegeneration. Our recent studies have identified microglia as novel regulators of neuronal activity and function, maintaining homeostatic levels of neuronal activation, thereby serving as brake pads for hyperexcitation. Preliminary evidence suggests this neuroprotective function may be altered in aging, potentially as a consequence of inflammatory microglia activation associated with aging and neurodegenerative disease. I hypothesize that microglia play a critical role in aberrant neuronal responses and dysfunction in the aging brain. In support of this idea, we found that microglia are able to regulate neuronal activity in an activity dependent manner by responding to ATP released during neuronal activation and metabolizing it into adenosine, thereby suppressing neuronal activity. We further found that age-associated increase in pro-inflammatory gene expression in microglia is associated with changes in this mechanism. To investigate my hypothesis, I will first elucidate the exact mechanisms of microglia-mediated neurosuppression, using the healthy adult striatum as a model. Microglia express the rate-limiting enzyme, CD39, which controls ATP to AMP degradation, while AMP to adenosine degradation is accomplished by CD73, which is expressed in microglia but also by striatal D2 medium spiny neurons. This suggests that microglia may either be independently sufficient to produce adenosine by expressing both CD39 and CD73, or this mechanism requires microglia-neuron interactions. To investigate this, we propose to generate transgenic mouse models to identify the relative contributions of CD73 found on microglia versus D2 medium spiny neurons. Microglial sufficiency of adenosine production may implicate this as a brain-wide mechanism of neuroprotection against aberrant neuronal activation. Additionally, to investigate the presynaptic and postsynaptic specificity of this microglial mechanism, we will use transgenic mouse models lacking adenosine receptor either on projection neurons or D1 medium spiny neurons. Prevention of adenosine-based neuronal activity inhibition on either the presynapse or postsynapse will recapitulate the effects seen with loss of microglial adenosine generation. Using these models, we will assess neuronal activation by immediate early gene expression, open field locomotor activity, and seizure susceptibility. We further demonstrated that expression of key genes involved in microglia driven neuronal activity modulation are downregulated in aged animals. However, this has yet to be specifically observed in microglia. Therefore, we proposed to perform gene expression analysis specifically in microglia of different brain regions and animals of different ages. This information is critical to our understanding of the cellular mechanisms by which microglia regulate neuronal function and may help to identify key regulators of this pathway, leading to the development of novel approaches for the treatment of age-associated disorders.

项目总结/摘要 这一建议提出了一种新的小胶质细胞控制的神经元活动反馈机制及其潜力 对衰老依赖性神经病理学的贡献。小鼠和人类的衰老与细胞的变化有关 神经元回路兴奋性和功能、癫痫发作易感性增加和神经变性。我们最近 研究已经确定小胶质细胞作为神经元活动和功能的新调节剂，维持稳态 水平的神经元激活，从而作为刹车垫过度兴奋。初步证据表明 这种神经保护功能可能随着年龄的增长而改变，这可能是炎症性小胶质细胞的结果。 与衰老和神经退行性疾病相关的活化。我假设小胶质细胞在 异常的神经反应和衰老大脑的功能障碍。为了支持这一观点，我们发现小胶质细胞 能够通过响应于ATP释放而以活动依赖性方式调节神经元活动， 神经元激活并将其代谢成腺苷，从而抑制神经元活性。我们进一步发现 小胶质细胞中促炎基因表达的年龄相关性增加与这种变化有关。 机制为了研究我的假设，我将首先阐明小胶质细胞介导的 神经抑制，使用健康成人纹状体作为模型。小胶质细胞表达限速酶CD 39， 其控制ATP至AMP的降解，而AMP至腺苷的降解由CD 73完成， 在小胶质细胞中表达，但也由纹状体D2中等多刺神经元表达。这表明小胶质细胞可能 通过表达CD 39和CD 73，或这种机制， 需要小胶质细胞和神经元的相互作用为了研究这一点，我们建议建立转基因小鼠模型， 鉴定在小胶质细胞上发现的CD 73相对于D2中等多棘神经元的相对贡献。小胶质 腺苷产生的充分性可能暗示这是一种全脑的神经保护机制， 异常的神经元激活此外，为了研究突触前和突触后特异性， 小胶质细胞机制，我们将使用缺乏腺苷受体的转基因小鼠模型， 神经元或D1中等多刺神经元。预防基于腺苷的神经元活性抑制， 突触前或突触后将重现小胶质细胞腺苷生成损失所见的效应。使用 在这些模型中，我们将通过即刻早期基因表达、旷场运动、 活动和癫痫易感性。我们进一步证明了参与小胶质细胞的关键基因的表达， 驱动的神经元活动调节在老年动物中下调。然而，这还有待于具体 在小胶质细胞中观察到。因此，我们提出了专门在小胶质细胞中进行基因表达分析， 不同的大脑区域和不同年龄的动物。这些信息对于我们理解细胞的 小胶质细胞调节神经元功能的机制，并可能有助于确定这一关键调节因子。 途径，导致开发用于治疗年龄相关疾病的新方法。