Glycosylation Analysis by Sheath-Flow SERS
通过鞘流 SERS 进行糖基化分析
基本信息
- 批准号:10312126
- 负责人:
- 金额:$ 19.2万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-01-01 至 2023-12-31
- 项目状态:已结题
- 来源:
- 关键词:Biological ProductsCarbohydratesChemical StructureChemicalsComplexComplex MixturesCouplingDevelopmentEventExhibitsGlycobiologyGlycopeptidesGlycoproteinsGoalsHereditary DiseaseHigh Pressure Liquid ChromatographyImmuneInflammatoryIsomerismLaboratoriesLinkLiquid ChromatographyMalignant NeoplasmsMass Spectrum AnalysisMethodologyMolecularMonitorMonosaccharidesNeurodegenerative DisordersPeptidesPharmaceutical PreparationsPlayPolysaccharidesPost-Translational Protein ProcessingPrognosisProtein GlycosylationProteinsPublic HealthPublishingReportingResolutionRoleSafetySamplingSiteStructureSurfaceTechniquesTechnologyTherapeuticTimeWorkanalogbasebiomarker developmentcostdetection limitdetection methoddetectordisease diagnosisglycosylationhuman diseaseinstrumentationmass spectrometernew technologynovelpathogenprotein functiontherapeutic proteintooltreatment response
项目摘要
Project Abstract
Protein glycosylation plays important roles in various human diseases, including cancer, inflammatory, and
neurodegenerative diseases. Glycoproteins, therefore, are important targets for disease diagnosis, prognosis,
and therapeutics. Correct glycosylation of therapeutic proteins is required for optimal efficacy and safety.
However, analysis of glycosylation is challenging due to the enormous complexity of glycan variability.
The current state of art technique is mass spectrometry (MS), which has been a powerful approach for
glycosylation analysis; however, there are classes of glycans that present challenges for MS analysis, such as
the numerous component monosaccharides with the same mass.
This project seeks to develop surface enhanced Raman scattering (SERS) as online detection method for
glycosylation analysis at the levels of small glycopeptides and released glycans. This proposal leverages
recent advances incorporating a novel SERS detector online with chemical separations for highly sensitive and
selective characterization. Published results have successfully shown online separation, identification and
quantification of phosphorylated monosaccharides, demonstrating the tremendous potential of SERS for
qualitative and quantitative analysis of carbohydrates that serve as glycans and glycopeptides.
The specific aims of this project are as follows:
Aim 1. Demonstrate the utility of the sheath-flow SERS detector for structural analysis of glycopeptides.
We will use a set of structurally similar glycopeptide analogues to demonstrate the capability of the
SERS flow detector for identification and quantification of glycopeptides.
Aim 2. Qualitative and quantitative analysis of glycans and peptides released from glycoproteins using
LC- SERS. This aim will provide evidence for the capability of our SERS technology to identify and
quantify glycans and glycopeptides with similar chemical structure (e.g. isomers) in complex mixtures,
demonstrating its potential, as a complementary technique to MS, for comprehensive glycosylation
analysis of the levels of glycopeptides and released glycans.
The development of new technologies that provide unique chemical specific information will provide an
alternative tool for increased coverage of the glycoproteome, facilitating the progress in glycobiology.
项目摘要
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Chemical conjugation to differentiate monosaccharides by Raman and surface enhanced Raman spectroscopy.
通过拉曼和表面增强拉曼光谱化学结合来区分单糖。
- DOI:10.1039/d2an01762h
- 发表时间:2023
- 期刊:
- 影响因子:0
- 作者:Schorr,HannahC;Schultz,ZacharyD
- 通讯作者:Schultz,ZacharyD
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Zachary Schultz其他文献
Zachary Schultz的其他文献
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{{ truncateString('Zachary Schultz', 18)}}的其他基金
Enhanced Raman Imaging of Ligand-Receptor Recognition
配体受体识别的增强拉曼成像
- 批准号:
10687237 - 财政年份:2015
- 资助金额:
$ 19.2万 - 项目类别:
Targeted TERS Investigations of Ligand-Receptor Binding
配体-受体结合的靶向 TERS 研究
- 批准号:
9406141 - 财政年份:2015
- 资助金额:
$ 19.2万 - 项目类别:
Enhanced raman imaging of ligand-receptor recognition
配体-受体识别的增强拉曼成像
- 批准号:
10596420 - 财政年份:2015
- 资助金额:
$ 19.2万 - 项目类别:
Enhanced Raman Imaging of Ligand-Receptor Recognition
配体受体识别的增强拉曼成像
- 批准号:
10491043 - 财政年份:2015
- 资助金额:
$ 19.2万 - 项目类别:
Targeted TERS Investigations of Ligand-Receptor Binding
配体-受体结合的靶向 TERS 研究
- 批准号:
9211336 - 财政年份:2015
- 资助金额:
$ 19.2万 - 项目类别:
Ultrasensitive Label-Free Flow Detector via Surface Enhanced Raman Scattering
通过表面增强拉曼散射的超灵敏无标记流量检测器
- 批准号:
8575713 - 财政年份:2013
- 资助金额:
$ 19.2万 - 项目类别:
Ultrasensitive Label-Free Flow Detector via Surface Enhanced Raman Scattering
通过表面增强拉曼散射的超灵敏无标记流量检测器
- 批准号:
8887351 - 财政年份:2013
- 资助金额:
$ 19.2万 - 项目类别:
Ultrasensitive Label-Free Flow Detector via Surface Enhanced Raman Scattering
通过表面增强拉曼散射的超灵敏无标记流量检测器
- 批准号:
8729504 - 财政年份:2013
- 资助金额:
$ 19.2万 - 项目类别:
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