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Role played by Acid Ion Sensing Channels in Peripheral Artery Disease

酸离子传感通道在外周动脉疾病中的作用

基本信息

批准号：
10318665
负责人：
Marc Peter Kaufman
金额：
$ 58.11万
依托单位：
PENNSYLVANIA STATE UNIV HERSHEY MED CTR
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-12-15 至 2024-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10318665
关键词：
ASIC channel Acids Animal Disease Models Animal Model Arrhythmia Arteries Attention Attenuated Blood Vessels Blood flow Brain Stem CRISPR/Cas technology Cardiac Cardiovascular system Chest Pain Contracts Coronary artery Disease Epidemiology Exercise Fiber Generations Goals Health Heart Heart Contractilities Heart Rate Hindlimb Hour Hydrogen-Ion Concentration Individual Intermittent Claudication Ischemia Kidney Knock-out Lactic acid Leg Legal patent Life Light Limb structure Metabolic Muscle Muscle Contraction Muscle Form Glycogen Phosphorylase Myocardial Myocardial Infarction Names Nerve Oxygen Pain Patients Pattern Peripheral Peripheral arterial disease Pharmacology Play Preparation Production Protein Isoforms Rattus Reflex action Role Signal Transduction Skeletal Muscle Small Interfering RNA Spinal Cord Stimulus Stroke Sympathetic Nervous System Technology Testing Thinness Time Trees Triceps Brachii Muscle Vascular blood supply Vascular resistance afferent nerve arm experimental study femoral artery heart rhythm high risk knock-down mechanical stimulus mutant neuromechanism normotensive pressure prevent receptor response sensor vasoconstriction

项目摘要

PROJECT SUMMARY/ABSTRACT A reflex arising from contracting hind limb skeletal muscles is an important neural mechanism that is responsible for causing the cardiovascular adjustments to exercise. These adjustments, which include increases in peripheral vascular resistance, cardiac contractility and rate, function to increase arterial blood flow and oxygen to the exercising muscles, and in turn support their ability to contract. This neural mechanism has been named the exercise pressor reflex and its afferent arm is comprised of group III and IV fibers whose endings are located in and near the muscle interstitium. In patients with peripheral artery disease (PAD) the exercise pressor reflex is exaggerated. The overall goal of the experiments proposed in this application is to shed light on the role played by lactic acid in evoking the exercise pressor reflex in both health and simulated peripheral artery disease. The proposed experiments will pay particular attention to Acid Sensing Ion Channels (ASIC) and will focus on the role that they play in evoking the exaggerated exercise pressor reflex in PAD. Lactic acid, produced by contracting skeletal muscles is believed to stimulate group III and IV afferents, signaling the spinal cord and brainstem that the arterial blood supply to working muscle is not meeting its metabolic demand. In the proposed experiments, we will pay particular attention to two important ASIC isoforms, namely ASIC1a and ASIC3. We will examine in decerebrated unanesthetized rats the responses to contraction of group III and IV muscle afferents both before and after either pharmacological blockade of the above receptors or after they have been “knocked down” with siRNA. The proposed experiments will also examine the responses to contraction of these afferents before and after knockdown of myophosphorylase in the triceps surae muscles. In addition the responses of group III and IV afferents to contraction in mutant rats whose ASIC3 has been functionally inactivated by CRISPR Cas 9 technology will be determined. The proposed experiments will be performed both in rats with freely perfused femoral arteries and in rats with femoral arteries that have been ligated for 72 hours before the start of the experiment. The latter preparation simulates the arterial blood flow patterns seen in patients with PAD and therefore serves as a useful animal model of this disease. The proposed experiments are anticipated to provide new information about metabolic factors that cause the exercise pressor reflex to be exaggerated in PAD.

项目总结/文摘