PAGES: Physical Activity Genomics, Epigenomics/transcriptomics Site

项目摘要 体力活动(PA)可预防或改善许多疾病，不运动排在第四位 全球死亡风险因素。PA的多种益处的分子机制还不是很清楚 明白了。体力活动分子传感器联盟(MoTrPAC)正在形成中 增进这一领域的知识。我们建议建立PAGE，一种体力活动基因组学， 表观基因组学/转录组学位点作为MoTrPAC的一个组成部分。佩奇将进行 对大鼠和人PA干预MoTrPAC样本的综合分析，有助于这些数据 公共数据库，帮助确定PA的候选分子传感器并阐明新的PA反应 机制，并帮助开发个人对PA反应的预测模型。PAGE化验网站： 位于西奈山的伊坎医学院、纽约基因组中心和布罗德研究所提供 支持这种大规模、全面的分子分析的基础设施、专业知识和经验 与PA相关的更改。Pages的目标是1.在第一年与MoTrPAC指导委员会合作 最终确定计划和协议；2.执行分析和分析，以帮助确定候选分子换能器 在大鼠模型中对PA的反应以及导致模型差异的途径，包括高深度 RNA-SEQ和全基因组亚硫酸盐测序(WGBS)，并补充其他分析类型，如 根据初步结果进行CHIP-SEQ、ATAC-SEQ；3.对人体进行全面的检测和分析 MoTrPAC临床研究组织样本，包括RNA-SEQ、WGBS、H3K27ac芯片-SEQ、ATAC-SEQ和WALL 基因组测序。4.与MoTrPAC协作分析来自Pages和其他MoTrPAC的数据 分析位点，以确定候选PA传感器和分子机制，并开发预测 PA能力和对培训的反应模型。Pages和MoTrPAC计划的成功将 转变对分子网络的洞察，将PA转化为健康，创造无与伦比的 全面的公共广播数据资源，可以为深入推进公共广播事业提供基础 防治人类许多重大疾病。

Project Summary Physical activity (PA) prevents or ameliorates a large number of diseases, and inactivity is the 4th leading global mortality risk factor. The molecular mechanisms responsible for the diverse benefits of PA are not well understood. The Molecular Transducers of Physical Activity Consortium (MoTrPAC) is being formed to advance knowledge in this area. We propose to establish PAGES, a Physical Activity Genomics, Epigenomics/transcriptomics Site as an integral component of the MoTrPAC. PAGES will conduct comprehensive analyses of the rat and human PA intervention MoTrPAC samples, contribute these data to public databases, help identify candidate molecular transducers of PA and elucidate new PA response mechanisms, and help develop predictive models of the individual response to PA. PAGES assay sites at Icahn School of Medicine at Mount Sinai, New York Genome Center and Broad Institute provide the infrastructure, expertise and experience to support this large scale, comprehensive analysis of molecular changes associated with PA. PAGES aims are to 1. Work with the MoTrPAC Steering Committee in Year 1 to finalize plans and protocols; 2. Perform assays and analyses to help Identify candidate molecular transducers of the response to PA in rat models and the pathways responsible for model differences, including high-depth RNA-seq and Whole Genome Bisulfite Sequencing (WGBS), supplemented by additional assay types such as ChIP-seq, ATAC-seq based on initial results; 3. Perform comprehensive assays and analyses of the human MoTrPAC clinical study tissue samples, including RNA-seq, WGBS, H3K27ac ChIP-seq, ATAC-seq and whole genome sequencing. 4. Collaborate with the MoTrPAC to analyze data from PAGES and other MoTrPAC analysis sites to identify candidate PA transducers and molecular mechanisms, and to develop predictive models of PA capacity and response to training. The success of PAGES and the MoTrPAC program will transform insight into the molecular networks that transduce PA into health, create an unparalleled comprehensive public PA data resource, and can provide the foundation for profound advances in the prevention and treatment of many major human diseases.