PAGES: Physical Activity Genomics, Epigenomics/transcriptomics Site

项目摘要 身体活动（PA）可以预防或改善多种疾病，而不活动是第四大疾病 全球死亡风险因素。PA的多种益处的分子机制尚不清楚 明白物理活动的分子传感器联盟（MoTrPAC）正在形成， 这方面的先进知识。我们建议建立PAGES，身体活动基因组学， 表观基因组学/转录组学网站作为MoTrPAC的组成部分。PAGES将进行 大鼠和人类PA干预MoTrPAC样品的综合分析，将这些数据贡献给 公共数据库，帮助识别PA的候选分子转换器和阐明新的PA响应 机制，并帮助开发个人对PA反应的预测模型。PAGES检测中心 西奈山伊坎医学院、纽约基因组中心和布罗德研究所提供了 基础设施，专业知识和经验，以支持这种大规模，全面的分子分析， 与PA相关的变化。页面的目标是1。在第一年与交通部指导委员会合作， 完成计划和议定书; 2.进行检测和分析，以帮助识别候选分子转换器 在大鼠模型中对PA的反应以及导致模型差异的途径，包括高深度 RNA-seq和全基因组亚硫酸氢盐测序（WGBS），辅以其他检测类型，如 ChIP-seq、ATAC-seq基于初步结果; 3.对人体进行全面的检测和分析 MoTrPAC临床研究组织样本，包括RNA-seq、WGBS、H3 K27 ac ChIP-seq、ATAC-seq和全 基因组测序4.与MoTrPAC合作，分析来自PAGES和其他MoTrPAC的数据 分析位点，以鉴定候选PA转换器和分子机制，并开发预测 巴勒斯坦权力机构的能力和对培训的反应模式。PAGES和MoTrPAC计划的成功将 将洞察力转化为分子网络，将ESPPA转化为健康，创造无与伦比的 全面的公共公共PA数据资源，并可以为 预防和治疗许多重大疾病。

Project Summary Physical activity (PA) prevents or ameliorates a large number of diseases, and inactivity is the 4th leading global mortality risk factor. The molecular mechanisms responsible for the diverse benefits of PA are not well understood. The Molecular Transducers of Physical Activity Consortium (MoTrPAC) is being formed to advance knowledge in this area. We propose to establish PAGES, a Physical Activity Genomics, Epigenomics/transcriptomics Site as an integral component of the MoTrPAC. PAGES will conduct comprehensive analyses of the rat and human PA intervention MoTrPAC samples, contribute these data to public databases, help identify candidate molecular transducers of PA and elucidate new PA response mechanisms, and help develop predictive models of the individual response to PA. PAGES assay sites at Icahn School of Medicine at Mount Sinai, New York Genome Center and Broad Institute provide the infrastructure, expertise and experience to support this large scale, comprehensive analysis of molecular changes associated with PA. PAGES aims are to 1. Work with the MoTrPAC Steering Committee in Year 1 to finalize plans and protocols; 2. Perform assays and analyses to help Identify candidate molecular transducers of the response to PA in rat models and the pathways responsible for model differences, including high-depth RNA-seq and Whole Genome Bisulfite Sequencing (WGBS), supplemented by additional assay types such as ChIP-seq, ATAC-seq based on initial results; 3. Perform comprehensive assays and analyses of the human MoTrPAC clinical study tissue samples, including RNA-seq, WGBS, H3K27ac ChIP-seq, ATAC-seq and whole genome sequencing. 4. Collaborate with the MoTrPAC to analyze data from PAGES and other MoTrPAC analysis sites to identify candidate PA transducers and molecular mechanisms, and to develop predictive models of PA capacity and response to training. The success of PAGES and the MoTrPAC program will transform insight into the molecular networks that transduce PA into health, create an unparalleled comprehensive public PA data resource, and can provide the foundation for profound advances in the prevention and treatment of many major human diseases.