Antibody and Bioresource

摘要 抗体和生物资源(ABR)核心是一个充满活力的设施，对基础和 中心的翻译使命，专注于可再生性研究的两个核心工具，特别是 抗体和细胞系。Core与研究人员合作开发新的单抗(MAb) 针对他们感兴趣的目标(S)，将工作在所需的应用程序(S)。当抗体不容易产生时 该设施在其他地方可用，特别是在MSK新开发的，通过以下方式提供对基本单抗的访问：1) 生产(体外)；2)纯化；3)结合(荧光和蛋白质)；和/或4)将它们裂解成 Fab或F(Ab‘)2片段。促进使用健康的细胞培养，这是产生 来自这些体外系统的可重现数据，ABR Core提供支原体检测服务。井 在MSK创建并存放到Core中的经过验证的细胞系被分发给各地的研究人员 这确保了癌症中心共享的工具是最高质量的。相关 还与接受者分享了有关经过管理的细胞系的信息和测试结果。这些包括，但不是 仅限于，该系列的描述和历史、参考资料、文化条件、独特特征、STR 用于鉴定人类细胞系的轮廓，细胞的图像，确认没有支原体 在培养、核型分析和适当的流式细胞术分析中均可检测到。在过去的拨款期间 在此期间，ABR Core为CCSG 10个项目中的7个项目的研究人员发表了50篇论文。

ABSTRACT The Antibody and BioResource (ABR) Core is a vibrant facility that contributes broadly to the basic and translational mission of the Center by focusing on two tools central to reproducibile research, specifically antibodies and cell lines. The Core works with researchers to develop new monoclonal antibodies (MAbs) against their target(s) of interest that will work in the required application(s). When antibodies are not readily available elsewhere, particularly newly developed at MSK, the facility provides access to essential MAbs by: 1) Producing (in vitro); 2) Purifying; 3) Conjugating (to fluorphores and proteins); and/or 4) Fragmenting them into Fab or F(Ab’)2 fragments. To promote the use of healthy cell cultures, which is central to generating reproducable data from these in vitro systems, the ABR Core offers a mycoplasma testing service. Well authenticated cell lines created at MSK and deposited into the Core are distributed to researchers around the world, which ensures the tools that the Cancer Center is sharing are of the highest quality. Relevant information and testing results on curated cell lines are also shared with recipients. These include, but are not limited to, a description and history of the line, references, culture conditions, unique characteristics, STR profiles for authentication purposes of human cell lines, images of the cells, confirmation that no mycoplasma was detectable in the culture, karyotyping, and appropriate flow cytometric analysis. During the past grant period the ABR Core has contributed to 50 publications for researchers from seven of the 10 CCSG Programs.