Antibody and Bioresource

抗体与生物资源

• 批准号: 10319500
• 负责人: Frances Weis-Garcia
• 金额: $ 29.29万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-20 至 2023-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10319500
• 关键词: Acholeplasma; Address; Antibodies; Basic Science; Binding; Biological; Cancer Center; Cancer Center Support Grant; Cell Culture Techniques; Cell Line; Characteristics; Clinical Research; Communities; Data; Deposition; Diagnosis; Ensure; Entomoplasma; Grant; Human Cell Line; Hybridomas; In Vitro; Karyotype determination procedure; Mollicutes; Monoclonal Antibodies; Mycoplasma; Proteins; Publications; Reagent; Recording of previous events; Reproducibility; Research; Research Personnel; Scientist; Services; Spiroplasma; System; Techniques; Test Result; Testing; Time; Translational Research; Work; cancer therapy; cellular imaging; cost effective; fluorophore; interest; programs; testing services; tool; translational goal

ABSTRACT The Antibody and BioResource (ABR) Core is a vibrant facility that contributes broadly to the basic and translational mission of the Center by focusing on two tools central to reproducibile research, specifically antibodies and cell lines. The Core works with researchers to develop new monoclonal antibodies (MAbs) against their target(s) of interest that will work in the required application(s). When antibodies are not readily available elsewhere, particularly newly developed at MSK, the facility provides access to essential MAbs by: 1) Producing (in vitro); 2) Purifying; 3) Conjugating (to fluorphores and proteins); and/or 4) Fragmenting them into Fab or F(Ab’)2 fragments. To promote the use of healthy cell cultures, which is central to generating reproducable data from these in vitro systems, the ABR Core offers a mycoplasma testing service. Well authenticated cell lines created at MSK and deposited into the Core are distributed to researchers around the world, which ensures the tools that the Cancer Center is sharing are of the highest quality. Relevant information and testing results on curated cell lines are also shared with recipients. These include, but are not limited to, a description and history of the line, references, culture conditions, unique characteristics, STR profiles for authentication purposes of human cell lines, images of the cells, confirmation that no mycoplasma was detectable in the culture, karyotyping, and appropriate flow cytometric analysis. During the past grant period the ABR Core has contributed to 50 publications for researchers from seven of the 10 CCSG Programs.

抽象的 抗体和生物资源 (ABR) 核心是一个充满活力的设施，为基础和生物资源做出了广泛贡献 该中心的转化使命是重点关注可重复性研究的两个核心工具，特别是 抗体和细胞系。 The Core 与研究人员合作开发新的单克隆抗体 (MAb) 针对他们感兴趣的目标，并将其用于所需的应用程序。当抗体不易产生时 该设施在其他地方可用，特别是 MSK 新开发的，通过以下方式提供基本单克隆抗体：1) 生产（体外）； 2）净化； 3) 缀合（荧光团和蛋白质）；和/或 4) 将它们分成 Fab 或 F(Ab’)2 片段。促进健康细胞培养物的使用，这是生成细胞的核心 ABR Core 提供支原体测试服务，可从这些体外系统中获得可重复的数据。出色地 在 MSK 创建并存入 Core 的经过验证的细胞系被分发给各地的研究人员 世界，这确保了癌症中心共享的工具具有最高质量。相关的 有关精选细胞系的信息和测试结果也会与接收者共享。这些包括但不包括 仅限于产品线的描述和历史、参考文献、文化条件、独特特征、STR 用于验证人类细胞系的配置文件、细胞图像、确认不存在支原体 在培养、核型分析和适当的流式细胞分析中可检测到。在过去的补助期间 在此期间，ABR 核心为来自 10 个 CCSG 项目中的 7 个项目的研究人员撰写了 50 篇出版物。