MONOCLONAL ANTIBODY

单克隆抗体

• 批准号: 8375213
• 负责人: Frances Weis-Garcia
• 金额: $ 15.81万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-01-09 至 2013-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8375213
• 关键词: Affinity Chromatography; Antigens; Biomedical Research; Cancer Center Support Grant; Cell Separation; Cells; Core Facility; Disease; Enzymes; Flow Cytometry; Generations; Goals; Hybridomas; Immunoelectron Microscopy; Immunofluorescence Immunologic; Immunoprecipitation; In Vitro; Infection; Malignant Neoplasms; Memorial Sloan-Kettering Cancer Center; Modification; Monoclonal Antibodies; Mycoplasma; Population; Production; Proteins; Recombinants; Research; Research Personnel; Research Support; Sampling; Services; Source; T-Cell Activation; Techniques; Testing; Western Blotting; cell bank; cellular imaging; cost; cytochemistry; disease diagnosis; in vivo; interest; milligram; programs; research study; tissue culture; tool

The Monoclonal Antibody Core Facility (MACF) provides the following four high quality services not only at a reasonable cost to investigators but also in a timely manner. 1. The generation of new MAbs / hybridomas to a specific antigen of interest. 2. MAb production (in vitro), purification and modification (including conjugation to fluorphores, biotion and enzymes and the fragmentation of MAbs). 3. Mycoplasma testing of tissue culture samples. 4. In vitro production of recombinant/secreted proteins. Monoclonal antibodies (MAbs) are essential tools used in most if not all fields of biomedical research, in the diagnosis of diseases, and in the treatment of diseases such as infections and cancer. The goal of the MACF is to support the research interests of MSKCC investigators by providing key services related to the production, purification and use of MAbs. Over the last 5 years demand for the MACF services has remained consistently high. From July 2005 through July 2006, 61 investigators from 9 out of 10 programs placed orders with the facility for any combination of the services noted above.

单克隆抗体核心设施（MACF）不仅提供以下四项高质量服务， 对调查人员来说费用合理，但也要及时。 1.产生针对感兴趣的特异性抗原的新的MAb/杂交瘤。 2.单克隆抗体生产（体外）、纯化和修饰（包括与荧光团缀合， 生物素和酶以及单克隆抗体的片段化）。 3.组织培养样本的支原体检测。 4.重组/分泌蛋白的体外生产。 单克隆抗体（MAb）是在生物医学研究的大多数（如果不是所有）领域中使用的基本工具， 疾病诊断和疾病如感染和癌症的治疗。的目标 MACF将通过提供与以下方面相关的关键服务来支持MSKCC研究人员的研究兴趣： MAb的生产、纯化和使用。 在过去的五年里，对MACF服务的需求一直很高。自2005年7月起 截至2006年7月，10个项目中有9个项目的61名调查人员向该设施订购了任何 上述服务的组合。