Antibody and Bioresource

抗体与生物资源

• 批准号: 10084816
• 负责人: Frances Weis-Garcia
• 金额: $ 29.29万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-20 至 2023-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10084816
• 关键词: Acholeplasma; Address; Antibodies; Basic Science; Binding; Biological; Cancer Center; Cancer Center Support Grant; Cell Culture Techniques; Cell Line; Characteristics; Cleaved cell; Clinical Research; Communities; Data; Deposition; Diagnosis; Ensure; Entomoplasma; Grant; Human Cell Line; Hybridomas; In Vitro; Karyotype determination procedure; Mission; Mollicutes; Monoclonal Antibodies; Mycoplasma; Proteins; Publications; Reagent; Recording of previous events; Reproducibility; Research; Research Personnel; Scientist; Services; Spiroplasma; System; Techniques; Test Result; Testing; Time; Translational Research; Work; cancer therapy; cellular imaging; cost effective; fluorophore; interest; programs; testing services; tool

ABSTRACT The Antibody and BioResource (ABR) Core is a vibrant facility that contributes broadly to the basic and translational mission of the Center by focusing on two tools central to reproducibile research, specifically antibodies and cell lines. The Core works with researchers to develop new monoclonal antibodies (MAbs) against their target(s) of interest that will work in the required application(s). When antibodies are not readily available elsewhere, particularly newly developed at MSK, the facility provides access to essential MAbs by: 1) Producing (in vitro); 2) Purifying; 3) Conjugating (to fluorphores and proteins); and/or 4) Fragmenting them into Fab or F(Ab’)2 fragments. To promote the use of healthy cell cultures, which is central to generating reproducable data from these in vitro systems, the ABR Core offers a mycoplasma testing service. Well authenticated cell lines created at MSK and deposited into the Core are distributed to researchers around the world, which ensures the tools that the Cancer Center is sharing are of the highest quality. Relevant information and testing results on curated cell lines are also shared with recipients. These include, but are not limited to, a description and history of the line, references, culture conditions, unique characteristics, STR profiles for authentication purposes of human cell lines, images of the cells, confirmation that no mycoplasma was detectable in the culture, karyotyping, and appropriate flow cytometric analysis. During the past grant period the ABR Core has contributed to 50 publications for researchers from seven of the 10 CCSG Programs.

摘要 抗体和生物资源（ABR）的核心是一个充满活力的设施，有助于广泛的基础和 该中心的翻译使命，重点是两个工具的核心，可再生的研究，具体 抗体和细胞系。核心与研究人员合作开发新的单克隆抗体（MAbs） 针对将在所需应用程序中工作的感兴趣的目标。当抗体不容易 该设施通过以下方式提供基本MAb：1） 产生（体外）; 2）纯化; 3）缀合（与荧光团和蛋白质）;和/或4）将它们片段化成 Fab或F（Ab '）2片段。促进健康细胞培养的使用，这是产生 ABR Core提供了支原体检测服务。以及 在MSK创建并存入核心的经认证的细胞系被分发给世界各地的研究人员。 这确保了癌症中心共享的工具是最高质量的。相关 还与接受者分享关于精选细胞系的信息和测试结果。这些包括但不 仅限于品系的描述和历史、参考文献、培养条件、独特特征、STR 用于人细胞系认证目的的概况，细胞图像，确认无支原体 在培养、核型分析和适当的流式细胞术分析中可检测到。在过去的赠款 在此期间，ABR核心已经为CCSG 10个项目中的7个项目的研究人员提供了50份出版物。