Georgia Comprehensive Metabolomics and Proteomics Unit for MoTrPAC

项目总结 佐治亚州MoTrPAC综合代谢和蛋白质组学单位(CMP-GA)是一个交叉性的 拥有先进的高通量化学分析和大数据能力的团队，开发全面的 体力活动的分子传感器图。调查小组在超灵敏、精确和 小分子、脂类和蛋白质的空间分辨分析。团队成员都有很强的学术素养 作为创新的、独立的科学家、核心实验室主任和综合体中的有效合作者 研究计划。仪器包括最先进的超高分辨率精确质量强场 Orbitrap串联质谱仪(MS)和超高效液相色谱(UPLC)； 通过5m分辨率的高通量多模式MS进行三维(3D)分子成像；独特的离子 用于化学鉴定的迁移率-质谱机学习方法；以及其他方法，如GC- Orbitrap，一维和二维高场(800 MHz)核磁共振波谱，电感耦合等离子体质谱，免疫分析，化学和 分析实验室通过美国国立卫生研究院支持的亚特兰大临床中心进行整合 和翻译科学研究所，并在埃默里杰出的研究环境中运作 和佐治亚理工学院(GA Tech)。《议定书》-GA有六个要素组织起来，以提供 1)在设计和实施MoTrPAC目标方面发挥领导作用；2)生物信息学、计算支持 并将数据交付到MoTrPAC数据协调中心；3)全球、有针对性和空间分辨率 代谢组学；4)明确化学鉴定新型分子传感器的代谢物取证； 5)创新的蛋白质组和化学蛋白质组技术以定义氧化还原蛋白质组内的转导， 泛素组、乙酰组、动态组和核蛋白质组；6)脂类的鉴定和定量 换能器。通过成功完成这些目标以及与MoTrPAC的合作 财团，CMP-GA的研究人员将提供公开可用的数据资源和分子换能器 将加强和加快对受物理影响的疾病和状况的机制研究的地图 活动。

PROJECT SUMMARY The Georgia Comprehensive Metabolomics and Proteomics Unit for MoTrPAC (CMP-GA) is a cross-cutting team with advanced high-throughput chemical analytics and big data capabilities to develop a comprehensive map of molecular transducers of physical activity. The investigative team excels in ultrasensitive, precise and spatially resolved analyses of small molecules, lipids, and proteins. The team members have strong academic records as innovative, independent scientists, core laboratory directors and effective collaborators in complex research initiatives. Instrumentation includes state-of-the-art ultra-high resolution accurate mass high-field Orbitrap tandem mass spectrometry (MS) and ultraperformance liquid chromatography (UPLC); three- dimensional (3-D) molecular imaging via high throughput multi-modal MS at 5 m resolution; unique ion mobility-mass spectrometry machine-learning approaches for chemical identifications; and other such as GC- Orbitrap, 1D and 2D high field (800 MHz) NMR spectroscopy, ICP-MS, immunoassays, chemical and enzymatic assays, etc. The analytical laboratories are integrated through the NIH-supported Atlanta Clinical and Translational Sciences Institute, and operate within the outstanding research environment of Emory University and the Georgia Institute of Technology (GA Tech). CMP-GA has six elements organized to provide 1) leadership in the design and implementation of MoTrPAC goals; 2) bioinformatics, computational support and data delivery to the MoTrPAC Data Coordinating Center; 3) global, targeted and spatially resolved metabolomics; 4) metabolite forensics for unequivocal chemical identification of novel molecular transducers; 5) innovative proteomic and chemoproteomic technologies to define transducers within the redox proteome, ubiquitinome, acetylome, kinome and nuclear proteome; and 6) identification and quantification of lipid transducers. Through the successful completion of these aims and collaboration with the MoTrPAC consortium, investigators of CMP-GA will deliver a publically-available data resource and molecular transducer map that will enhance and accelerate mechanistic research on diseases and conditions affected by physical activity.