Building transgenic tools in Acomys cahirinus, an emerging model for mammalian regenerative biology and healthy aging
Building transgenic tools in Acomys cahirinus, an emerging model for mammalian regenerative biology and healthy aging
批准号:
10327728
负责人:
Kathleen Joyce Millen
金额:
$23.56万
依托单位国家:
美国
项目类别:
财政年份:
2021
资助国家:
美国
项目状态:
已结题
起止时间:
2021-01-15 至 2023-12-31
关键词:
AcomysAddressAdultAffectAgingAmbystomaAnimal ModelAnimalsAntibodiesArchitectureBiologicalBiological ModelsBiologyBiology of AgingBudgetsBypassCartilageCell LineCellsChimera organismChronicCicatrixClustered Regularly Interspaced Short Palindromic RepeatsCommunitiesComplementDermalDevelopmentDiseaseDorsalEarEmbryoEpitopesFailureFemaleGene ExpressionGene Transfer TechniquesGenerationsGeneticGenetic ModelsGenetic ResearchGenomeGenome engineeringHealthHigh Fat DietHouse miceHousingHumanHuman BiologyImageImplantIn SituIn VitroInfectionInflammationInfrastructureInjectionsInner Cell MassInvestigationKidneyKnowledgeLentivirusMammalian OviductsMammalsMethodologyMethodsModelingMolecularMusNatural regenerationOrganOrganismOvulationPancreasPeriodicityPersonsPhysiologicalPilot ProjectsPropertyProtocols documentationPseudopregnancyRattusReagentRecording of previous eventsRegenerative MedicineRegenerative capacityReproductive PhysiologyResearch PersonnelResourcesRibonucleoproteinsRodentSkinStudy modelsSuperovulationTechnologyTestingTimeTissuesTransgenic AnimalsTransgenic OrganismsTraumaTraumatic injuryUnited States Department of AgricultureVirus DiseasesWorkXenopusZebrafishbaseblastocystbody systemcell typedesigneconomic costembryonic stem cellexperimental studyexternal ear auriclegene functiongenome editingglobal healthhealinghealthy agingimplantationimprovedin vivoneonatenew technologynovel therapeutic interventionnovel therapeuticsnucleic acid deliverypregnantregeneration modelregenerativeregenerative biologyresponsesingle-cell RNA sequencingspecies differencesuccesstoolwound healing
中文摘要
项目总结
英文摘要
Project Summary
Many of the major biological discoveries of the 20th century were made using very few model species. They
were chosen for historical tractability, rather than biological attributes relevant to critical biological questions or
relevance to pressing global health issues. However, the advent of new sequencing and genome engineering
technologies makes establishment of new genetic models feasible. History has demonstrated that the success
of model organisms is self-perpetuating: as the community of researchers grows, new methodologies and
resources are developed and shared, and the body of specific knowledge and access to powerful tools to
manipulate, observe and experiment upon these model organisms further lowers the bar to entry so that the
cycle can repeat (18). Here we propose to develop transgenic infrastructure for Acomys cahinirus an emerging
model of adult mammalian non-fibrotic regenerative healing and healthy aging. We have propose a methodical
comprehensive and feasible plan to achieve transgenesis in Acomys. We first propose the generation of
interspecies Mus musculus Acomys leveraging Acomys ES cells which we have generated and CRISPR
tagged with a fluorescent marker. We will then work to generate whole animal transgenic Acomys animals
using a variety of ex vivo and in situ methodologies. Acomys transgenesis will unlock the genetics of the
extraordinary regenerative biology of Acomys certain to yield novel therapeutic approaches to human health
and disease.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
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财政年份:2012
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依托单位:
Congenital brain malformations caused by aberrant head mesenchymal signaling
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财政年份:2012
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依托单位:
Congenital brain malformations caused by aberrant head mesenchymal signaling
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Dorsal midline patterning in the vertebrate CNS
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Dorsal midline patterning in the vertebrate CNS
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海外基金