Congenital brain malformations caused by aberrant head mesenchymal signaling

头部间质信号异常引起的先天性脑畸形

• 批准号: 9086446
• 负责人: Kathleen Joyce Millen
• 金额: $ 51.11万
• 依托单位: SEATTLE CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-15 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9086446
• 关键词: Affect; Biological Assay; Biology; Brain; Breeding; Candidate Disease Gene; Cerebellum; Clinical; Congenital Abnormality; Custom; Dandy-Walker Syndrome; Defect; Development; Developmental Gene; Diagnosis; Diagnostic; Disease; Electroporation; Embryo; FOXC1 gene; Family; Future; Gene Transfer Techniques; Genes; Genetic Heterogeneity; Genetic screening method; Goals; Growth; Head; Human; Hydrocephalus; In Vitro; Informatics; Lasers; Lead; Lip structure; Live Birth; Medical Genetics; Meningeal; Meninges; Mesenchymal; Mesenchyme; Methods; Molecular; Molecular Biology; Motor; Mus; Neurodevelopmental Disorder; Pathogenesis; Pathway interactions; Patients; Phenocopy; Phenotype; Posterior Fossa; RNA Interference; Regulation; Role; Scientist; Signal Pathway; Signal Transduction; Signaling Molecule; Slice; Structural Congenital Anomalies; Structural defect; System; Testing; Transgenic Mice; Ventricular; Walkers; base; brain malformation; brain pathway; clinical phenotype; cohort; design; developmental genetics; gene discovery; gene interaction; genome-wide; improved; in vivo; innovation; interdisciplinary approach; malformation; mouse model; mutant; neurodevelopment; novel; prognostic; prognostic tool; programs; research study; signal processing; transcription factor

DESCRIPTION (provided by applicant): This application describes an interdisciplinary approach involving basic and clinical scientists employing new and innovative informatic, genetic and developmental strategies to identify the underlying pathogenesis and causative genes for Dandy-Walker malformation, the most common structural malformation of the cerebellum. Dandy-Walker malformation is common, affecting 1/3000 live births and causes significant motor and intellectual delay and yet is poorly understood. Our group has identified the only 2 characterized loci for this clinically and genetically heterogeneous birth defect. Our analysis of mouse models has lead us to the hypothesis that disruption of mesenchymal signaling to the developing cerebellum is critical to the developmental pathogenesis of this birth defect. The recognition that the meninges is a critical regulator of CNS development is a recent paradigm shift in the field of neurodevelopment and the basic biology and molecular pathways of these interactions is not known. Further, it has become apparent that disrupted meningeal signaling underlies not only the significant clinical phenotypes of posterior fossa disorders such as Dandy-Walker, but has broad implications for the pathogenesis of large group of neurodevelopmental disorders that also involve meningeal signaling including ACC and others. The experiments outlined in this proposal are designed to identify pathways and mechanisms for posterior fossa mesenchymal regulation of cerebellar development, using Foxc1, the most recently identified Dandy-Walker gene, as an entry point. Aims 1-3 use novel in vitro and in vivo assays including explant culture, electroporation, RNAi and BAC transgenesis together with extensive informatic analyses to identify and validate the signaling pathways from the posterior fossa to the adjacent developing cerebellum which modulate Dandy-Walker related phenotypes in mouse models. In Aim 4 we will then sequence the best Dandy-Walker candidates from the first 3 Aims, in a cohort of human Dandy-Walker patients to identify new disease-causative genes. Together these synergistic mouse and human experiments will define new biology regarding mesenchymal control of neural development and identify new DWM genes, which will immediately improve diagnosis for affected families and will be essential for future prognostic studies.

描述(申请人提供)：本申请描述了一种跨学科的方法，涉及基础和临床科学家使用新的和创新的信息学、遗传学和发展战略，以确定Dandy-Walker畸形的潜在发病机制和致病基因，Dandy-Walker畸形是最常见的小脑结构畸形。Dandy-Walker畸形很常见，影响1/3000的活产儿，并导致显著的运动和智力迟缓，但人们对此知之甚少。我们小组已经确定了这种临床和遗传异质性出生缺陷的仅2个特征基因座。我们对小鼠模型的分析使我们得出这样的假设，即间质信号对发育中的小脑的干扰是这种出生缺陷的发育发病机制的关键。认识到脑膜是中枢神经系统发育的关键调节因子是神经发育领域最近的一个范式转变，这些相互作用的基本生物学和分子途径尚不清楚。此外，很明显，脑膜信号传导中断不仅是Dandy-Walker等后颅窝疾病重要临床表型的基础，而且对包括ACC和其他脑膜信号在内的一大组神经发育障碍的发病机制也具有广泛的意义。这项建议中概述的实验旨在以最近发现的Dandy-Walker基因Foxc1为切入点，确定后颅窝间充质调节小脑发育的途径和机制。AIMS 1-3使用新的体外和体内分析方法，包括外植体培养、电穿孔、RNAi和BAC转基因，并结合广泛的信息学分析，在小鼠模型中识别和验证从后颅窝到邻近发育中的小脑的信号通路。在目标4中，我们将在人类丹迪-沃克患者队列中对前3个目标中最好的丹迪-沃克候选基因进行排序，以识别新的致病基因。这些协同的小鼠和人类实验将定义关于间充质控制神经发育的新生物学，并识别新的DWM基因，这将立即改善对受影响家庭的诊断，并将对未来的预后研究至关重要。